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dc.creatorĐokić, Vitomir
dc.creatorBlaga, Radu
dc.creatorRinaldi, Laura
dc.creatorLe Roux, Delphine
dc.creatorDucry, Tamara
dc.creatorMaurelli, Maria Paola
dc.creatorPerret, Catherine
dc.creatorĐurković-Đaković, Olgica
dc.creatorCringoli, Giuseppe
dc.creatorBoireau, Pascal
dc.date.accessioned2021-04-20T12:36:49Z
dc.date.available2021-04-20T12:36:49Z
dc.date.issued2014
dc.identifier.issn0014-4894
dc.identifier.urihttp://rimi.imi.bg.ac.rs/handle/123456789/532
dc.description.abstractOocysts of Toxoplasma gondii represent one of the most common environmental contaminants causing the zoonotic infection toxoplasmosis. The aim of the present study was to compare the Mini-FLOTAC device with traditional cell counting plates (Kova Slide) for the detection of T. gondii oocysts from feline feces. Two types of experiments were performed: (i) purified oocysts were counted in different dilutions and (ii) specific pathogen free T. gondii-negative cat feces was inoculated with numbers of purified oocysts and counting was performed directly from feces. Our analysis showed a thousand times higher sensitivity of Mini-FLOTAC (5 x 10(2) oocysts) compared to Kova Slide (5 x 10(5) oocysts). Also, when compared by McNemar's test, counting of the purified oocysts showed a higher sensitivity of Mini-FLOTAC compared to Kova Slide, for a dilution of 10(3) oocysts/ml (chi(2) = 6.1; P lt 0.05). A better sensitivity was also found with Mini-FLOTAC in dilutions of 10(5) and 10(4) oocysts/ml, when counted from feces (chi(2) = 4.2 and 8.1, respectively, P lt 0.05). Our results show that Mini-FLOTAC is more sensitive than traditional methods of T. gondii oocysts detection and quantification is more accurate. Furthermore, Mini-FLOTAC simplicity and cost effectiveness allow it to be used with light microscopes in any laboratory or field conditions. We therefore recommend its use for regular screening. Further studies are needed to validate Mini-FLOTAC for the detection of oocysts in soil and water samples in field conditions.en
dc.publisherAcademic Press Inc Elsevier Science, San Diego
dc.rightsrestrictedAccess
dc.sourceExperimental Parasitology
dc.subjectToxoplasma gondiien
dc.subjectOocystsen
dc.subjectDetectionen
dc.subjectMini-FLOTACen
dc.subjectKova slideen
dc.titleMini-FLOTAC for counting Toxoplasma gondii oocysts from cat feces - Comparison with cell counting platesen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage71
dc.citation.other147: 67-71
dc.citation.rankM22
dc.citation.spage67
dc.citation.volume147
dc.identifier.doi10.1016/j.exppara.2014.10.009
dc.identifier.pmid25448359
dc.identifier.scopus2-s2.0-84910613600
dc.identifier.wos000346944200010
dc.type.versionpublishedVersion


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Приказ основних података о документу