Oocysts of Toxoplasma gondii represent one of the most common environmental contaminants causing the zoonotic infection toxoplasmosis. The aim of the present study was to compare the Mini-FLOTAC device with traditional cell counting plates (Kova Slide) for the detection of T. gondii oocysts from feline feces. Two types of experiments were performed: (i) purified oocysts were counted in different dilutions and (ii) specific pathogen free T. gondii-negative cat feces was inoculated with numbers of purified oocysts and counting was performed directly from feces. Our analysis showed a thousand times higher sensitivity of Mini-FLOTAC (5 x 10(2) oocysts) compared to Kova Slide (5 x 10(5) oocysts). Also, when compared by McNemar's test, counting of the purified oocysts showed a higher sensitivity of Mini-FLOTAC compared to Kova Slide, for a dilution of 10(3) oocysts/ml (chi(2) = 6.1; P lt 0.05). A better sensitivity was also found with Mini-FLOTAC in dilutions of 10(5) and 10(4) oocysts/ml,... when counted from feces (chi(2) = 4.2 and 8.1, respectively, P lt 0.05). Our results show that Mini-FLOTAC is more sensitive than traditional methods of T. gondii oocysts detection and quantification is more accurate. Furthermore, Mini-FLOTAC simplicity and cost effectiveness allow it to be used with light microscopes in any laboratory or field conditions. We therefore recommend its use for regular screening. Further studies are needed to validate Mini-FLOTAC for the detection of oocysts in soil and water samples in field conditions.
TY - JOUR
AU - Đokić, Vitomir
AU - Blaga, Radu
AU - Rinaldi, Laura
AU - Le Roux, Delphine
AU - Ducry, Tamara
AU - Maurelli, Maria Paola
AU - Perret, Catherine
AU - Đurković-Đaković, Olgica
AU - Cringoli, Giuseppe
AU - Boireau, Pascal
PY - 2014
UR - http://rimi.imi.bg.ac.rs/handle/123456789/532
AB - Oocysts of Toxoplasma gondii represent one of the most common environmental contaminants causing the zoonotic infection toxoplasmosis. The aim of the present study was to compare the Mini-FLOTAC device with traditional cell counting plates (Kova Slide) for the detection of T. gondii oocysts from feline feces. Two types of experiments were performed: (i) purified oocysts were counted in different dilutions and (ii) specific pathogen free T. gondii-negative cat feces was inoculated with numbers of purified oocysts and counting was performed directly from feces. Our analysis showed a thousand times higher sensitivity of Mini-FLOTAC (5 x 10(2) oocysts) compared to Kova Slide (5 x 10(5) oocysts). Also, when compared by McNemar's test, counting of the purified oocysts showed a higher sensitivity of Mini-FLOTAC compared to Kova Slide, for a dilution of 10(3) oocysts/ml (chi(2) = 6.1; P lt 0.05). A better sensitivity was also found with Mini-FLOTAC in dilutions of 10(5) and 10(4) oocysts/ml, when counted from feces (chi(2) = 4.2 and 8.1, respectively, P lt 0.05). Our results show that Mini-FLOTAC is more sensitive than traditional methods of T. gondii oocysts detection and quantification is more accurate. Furthermore, Mini-FLOTAC simplicity and cost effectiveness allow it to be used with light microscopes in any laboratory or field conditions. We therefore recommend its use for regular screening. Further studies are needed to validate Mini-FLOTAC for the detection of oocysts in soil and water samples in field conditions.
PB - Academic Press Inc Elsevier Science, San Diego
T2 - Experimental Parasitology
T1 - Mini-FLOTAC for counting Toxoplasma gondii oocysts from cat feces - Comparison with cell counting plates
EP - 71
SP - 67
VL - 147
DO - 10.1016/j.exppara.2014.10.009
ER -
@article{
author = "Đokić, Vitomir and Blaga, Radu and Rinaldi, Laura and Le Roux, Delphine and Ducry, Tamara and Maurelli, Maria Paola and Perret, Catherine and Đurković-Đaković, Olgica and Cringoli, Giuseppe and Boireau, Pascal",
year = "2014",
abstract = "Oocysts of Toxoplasma gondii represent one of the most common environmental contaminants causing the zoonotic infection toxoplasmosis. The aim of the present study was to compare the Mini-FLOTAC device with traditional cell counting plates (Kova Slide) for the detection of T. gondii oocysts from feline feces. Two types of experiments were performed: (i) purified oocysts were counted in different dilutions and (ii) specific pathogen free T. gondii-negative cat feces was inoculated with numbers of purified oocysts and counting was performed directly from feces. Our analysis showed a thousand times higher sensitivity of Mini-FLOTAC (5 x 10(2) oocysts) compared to Kova Slide (5 x 10(5) oocysts). Also, when compared by McNemar's test, counting of the purified oocysts showed a higher sensitivity of Mini-FLOTAC compared to Kova Slide, for a dilution of 10(3) oocysts/ml (chi(2) = 6.1; P lt 0.05). A better sensitivity was also found with Mini-FLOTAC in dilutions of 10(5) and 10(4) oocysts/ml, when counted from feces (chi(2) = 4.2 and 8.1, respectively, P lt 0.05). Our results show that Mini-FLOTAC is more sensitive than traditional methods of T. gondii oocysts detection and quantification is more accurate. Furthermore, Mini-FLOTAC simplicity and cost effectiveness allow it to be used with light microscopes in any laboratory or field conditions. We therefore recommend its use for regular screening. Further studies are needed to validate Mini-FLOTAC for the detection of oocysts in soil and water samples in field conditions.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Experimental Parasitology",
title = "Mini-FLOTAC for counting Toxoplasma gondii oocysts from cat feces - Comparison with cell counting plates",
pages = "71-67",
volume = "147",
doi = "10.1016/j.exppara.2014.10.009"
}
Đokić, V., Blaga, R., Rinaldi, L., Le Roux, D., Ducry, T., Maurelli, M. P., Perret, C., Đurković-Đaković, O., Cringoli, G.,& Boireau, P.. (2014). Mini-FLOTAC for counting Toxoplasma gondii oocysts from cat feces - Comparison with cell counting plates. in Experimental Parasitology
Academic Press Inc Elsevier Science, San Diego., 147, 67-71.
https://doi.org/10.1016/j.exppara.2014.10.009
Đokić V, Blaga R, Rinaldi L, Le Roux D, Ducry T, Maurelli MP, Perret C, Đurković-Đaković O, Cringoli G, Boireau P. Mini-FLOTAC for counting Toxoplasma gondii oocysts from cat feces - Comparison with cell counting plates. in Experimental Parasitology. 2014;147:67-71.
doi:10.1016/j.exppara.2014.10.009 .
Đokić, Vitomir, Blaga, Radu, Rinaldi, Laura, Le Roux, Delphine, Ducry, Tamara, Maurelli, Maria Paola, Perret, Catherine, Đurković-Đaković, Olgica, Cringoli, Giuseppe, Boireau, Pascal, "Mini-FLOTAC for counting Toxoplasma gondii oocysts from cat feces - Comparison with cell counting plates" in Experimental Parasitology, 147 (2014):67-71,
https://doi.org/10.1016/j.exppara.2014.10.009 . .
