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Skip regulates TGF- β 1-induced extracellular matrix degrading proteases expression in human PC-3 prostate cancer cells
dc.creator | Villar, Victor | |
dc.creator | Kocić, Jelena | |
dc.creator | Santibanez, Juan F. | |
dc.date.accessioned | 2021-04-20T12:35:12Z | |
dc.date.available | 2021-04-20T12:35:12Z | |
dc.date.issued | 2013 | |
dc.identifier.issn | 2090-3111 | |
dc.identifier.uri | http://rimi.imi.bg.ac.rs/handle/123456789/506 | |
dc.description.abstract | Purpose. To determine whether Ski-interacting protein (SKIP) regulates TGF-β1-stimulated expression of urokinase-type plasminogen activator (uPA), matrix metalloproteinase-9 (MMP-9), and uPA Inhibitor (PAI-1) in the androgen-independent human prostate cancer cell model. Materials and Methods. PC-3 prostate cancer cell line was used. The role of SKIP was evaluated using synthetic small interference RNA (siRNA) compounds. The expression of uPA, MMP-9, and PAI-1 was evaluated by zymography assays, RT-PCR, and promoter transactivation analysis. Results. In PC-3 cells TGF-β1 treatment stimulated uPA, PAI-1, and MMP-9 expressions. The knockdown of SKIP in PC-3 cells enhanced the basal level of uPA, and TGF-β1 treatment inhibited uPA production. Both PAI-1 and MMP-9 production levels were increased in response to TGF-β1. The ectopic expression of SKIP inhibited both TGF-β1-induced uPA and MMP-9 promoter transactivation, while PAI-1 promoter response to the factor was unaffected. Conclusions. SKIP regulates the expression of uPA, PAI-1, and MMP-9 stimulated by TGF-β1 in PC-3 cells. Thus, SKIP is implicated in the regulation of extracellular matrix degradation and can therefore be suggested as a novel therapeutic target in prostate cancer treatment. © 2013 Victor Villar et al. | en |
dc.publisher | Hindawi Limited | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175062/RS// | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.source | Prostate Cancer | |
dc.title | Skip regulates TGF- β 1-induced extracellular matrix degrading proteases expression in human PC-3 prostate cancer cells | en |
dc.type | article | |
dc.rights.license | BY | |
dc.citation.other | 2013: - | |
dc.citation.volume | 2013 | |
dc.identifier.doi | 10.1155/2013/398253 | |
dc.identifier.fulltext | http://rimi.imi.bg.ac.rs/bitstream/id/385/503.pdf | |
dc.identifier.scopus | 2-s2.0-84982317945 | |
dc.type.version | publishedVersion |