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Skip regulates TGF- β 1-induced extracellular matrix degrading proteases expression in human PC-3 prostate cancer cells

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2013
503.pdf (2.459Mb)
Аутори
Villar, Victor
Kocić, Jelena
Santibanez, Juan
Чланак у часопису (Објављена верзија)
Метаподаци
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Апстракт
Purpose. To determine whether Ski-interacting protein (SKIP) regulates TGF-β1-stimulated expression of urokinase-type plasminogen activator (uPA), matrix metalloproteinase-9 (MMP-9), and uPA Inhibitor (PAI-1) in the androgen-independent human prostate cancer cell model. Materials and Methods. PC-3 prostate cancer cell line was used. The role of SKIP was evaluated using synthetic small interference RNA (siRNA) compounds. The expression of uPA, MMP-9, and PAI-1 was evaluated by zymography assays, RT-PCR, and promoter transactivation analysis. Results. In PC-3 cells TGF-β1 treatment stimulated uPA, PAI-1, and MMP-9 expressions. The knockdown of SKIP in PC-3 cells enhanced the basal level of uPA, and TGF-β1 treatment inhibited uPA production. Both PAI-1 and MMP-9 production levels were increased in response to TGF-β1. The ectopic expression of SKIP inhibited both TGF-β1-induced uPA and MMP-9 promoter transactivation, while PAI-1 promoter response to the factor was unaffected. Conclusions. ...SKIP regulates the expression of uPA, PAI-1, and MMP-9 stimulated by TGF-β1 in PC-3 cells. Thus, SKIP is implicated in the regulation of extracellular matrix degradation and can therefore be suggested as a novel therapeutic target in prostate cancer treatment. © 2013 Victor Villar et al.

Извор:
Prostate Cancer, 2013, 2013
Издавач:
  • Hindawi Limited
Финансирање / пројекти:
  • Регенеративни и модулаторни потенцијал адултних матичних ћелија (RS-175062)

DOI: 10.1155/2013/398253

ISSN: 2090-3111

Scopus: 2-s2.0-84982317945
[ Google Scholar ]
7
URI
http://rimi.imi.bg.ac.rs/handle/123456789/506
Колекције
  • Radovi istraživača / Researchers' publications
Институција/група
Institut za medicinska istraživanja
TY  - JOUR
AU  - Villar, Victor
AU  - Kocić, Jelena
AU  - Santibanez, Juan
PY  - 2013
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/506
AB  - Purpose. To determine whether Ski-interacting protein (SKIP) regulates TGF-β1-stimulated expression of urokinase-type plasminogen activator (uPA), matrix metalloproteinase-9 (MMP-9), and uPA Inhibitor (PAI-1) in the androgen-independent human prostate cancer cell model. Materials and Methods. PC-3 prostate cancer cell line was used. The role of SKIP was evaluated using synthetic small interference RNA (siRNA) compounds. The expression of uPA, MMP-9, and PAI-1 was evaluated by zymography assays, RT-PCR, and promoter transactivation analysis. Results. In PC-3 cells TGF-β1 treatment stimulated uPA, PAI-1, and MMP-9 expressions. The knockdown of SKIP in PC-3 cells enhanced the basal level of uPA, and TGF-β1 treatment inhibited uPA production. Both PAI-1 and MMP-9 production levels were increased in response to TGF-β1. The ectopic expression of SKIP inhibited both TGF-β1-induced uPA and MMP-9 promoter transactivation, while PAI-1 promoter response to the factor was unaffected. Conclusions. SKIP regulates the expression of uPA, PAI-1, and MMP-9 stimulated by TGF-β1 in PC-3 cells. Thus, SKIP is implicated in the regulation of extracellular matrix degradation and can therefore be suggested as a novel therapeutic target in prostate cancer treatment. © 2013 Victor Villar et al.
PB  - Hindawi Limited
T2  - Prostate Cancer
T1  - Skip regulates TGF- β 1-induced extracellular matrix degrading proteases expression in human PC-3 prostate cancer cells
VL  - 2013
DO  - 10.1155/2013/398253
ER  - 
@article{
author = "Villar, Victor and Kocić, Jelena and Santibanez, Juan",
year = "2013",
abstract = "Purpose. To determine whether Ski-interacting protein (SKIP) regulates TGF-β1-stimulated expression of urokinase-type plasminogen activator (uPA), matrix metalloproteinase-9 (MMP-9), and uPA Inhibitor (PAI-1) in the androgen-independent human prostate cancer cell model. Materials and Methods. PC-3 prostate cancer cell line was used. The role of SKIP was evaluated using synthetic small interference RNA (siRNA) compounds. The expression of uPA, MMP-9, and PAI-1 was evaluated by zymography assays, RT-PCR, and promoter transactivation analysis. Results. In PC-3 cells TGF-β1 treatment stimulated uPA, PAI-1, and MMP-9 expressions. The knockdown of SKIP in PC-3 cells enhanced the basal level of uPA, and TGF-β1 treatment inhibited uPA production. Both PAI-1 and MMP-9 production levels were increased in response to TGF-β1. The ectopic expression of SKIP inhibited both TGF-β1-induced uPA and MMP-9 promoter transactivation, while PAI-1 promoter response to the factor was unaffected. Conclusions. SKIP regulates the expression of uPA, PAI-1, and MMP-9 stimulated by TGF-β1 in PC-3 cells. Thus, SKIP is implicated in the regulation of extracellular matrix degradation and can therefore be suggested as a novel therapeutic target in prostate cancer treatment. © 2013 Victor Villar et al.",
publisher = "Hindawi Limited",
journal = "Prostate Cancer",
title = "Skip regulates TGF- β 1-induced extracellular matrix degrading proteases expression in human PC-3 prostate cancer cells",
volume = "2013",
doi = "10.1155/2013/398253"
}
Villar, V., Kocić, J.,& Santibanez, J.. (2013). Skip regulates TGF- β 1-induced extracellular matrix degrading proteases expression in human PC-3 prostate cancer cells. in Prostate Cancer
Hindawi Limited., 2013.
https://doi.org/10.1155/2013/398253
Villar V, Kocić J, Santibanez J. Skip regulates TGF- β 1-induced extracellular matrix degrading proteases expression in human PC-3 prostate cancer cells. in Prostate Cancer. 2013;2013.
doi:10.1155/2013/398253 .
Villar, Victor, Kocić, Jelena, Santibanez, Juan, "Skip regulates TGF- β 1-induced extracellular matrix degrading proteases expression in human PC-3 prostate cancer cells" in Prostate Cancer, 2013 (2013),
https://doi.org/10.1155/2013/398253 . .

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