Here we investigate the influences of non-thermal atmospheric plasma on human mesenchymal stem cells isolated from periodontal ligament (hPDL-MSCs). A specially redesigned plasma needle was used as the source of low-temperature plasma and its effects on different hPDL-MSC functions were investigated. Cell cultures were obtained from extracted normal impacted third molars and characterized for their phenotype and multi-potential differentiation. The hPDL-MSCs possessed all the typical MSC properties, including clonogenic ability, high proliferation rate, specific phenotype and multilineage differentiation. The data regarding the interaction of plasma with hPDL-MSCs demonstrated that plasma treatment inhibited the migration of hPDL-MSCs and induced some detachment, while not affecting their viability. Additionally, plasma significantly attenuated hPDL-MSCs' proliferation, but promoted their osteogenic differentiation. The results of this study indicated that a non-thermal plasma offers s...pecific activity with non-destructive properties that can be advantageous for future dental applications.
Source:
Journal of Physics D-Applied Physics, 2013, 46, 34
TY - JOUR
AU - Miletić, Maja
AU - Mojsilović, S.
AU - Okić-Đorđević, Ivana
AU - Maletić, Dejan
AU - Puač, Nevena
AU - Lazović, Saša
AU - Malović, Gordana
AU - Milenković, P.
AU - Petrović, Zoran Lj.
AU - Bugarski, Diana
PY - 2013
UR - http://rimi.imi.bg.ac.rs/handle/123456789/487
AB - Here we investigate the influences of non-thermal atmospheric plasma on human mesenchymal stem cells isolated from periodontal ligament (hPDL-MSCs). A specially redesigned plasma needle was used as the source of low-temperature plasma and its effects on different hPDL-MSC functions were investigated. Cell cultures were obtained from extracted normal impacted third molars and characterized for their phenotype and multi-potential differentiation. The hPDL-MSCs possessed all the typical MSC properties, including clonogenic ability, high proliferation rate, specific phenotype and multilineage differentiation. The data regarding the interaction of plasma with hPDL-MSCs demonstrated that plasma treatment inhibited the migration of hPDL-MSCs and induced some detachment, while not affecting their viability. Additionally, plasma significantly attenuated hPDL-MSCs' proliferation, but promoted their osteogenic differentiation. The results of this study indicated that a non-thermal plasma offers specific activity with non-destructive properties that can be advantageous for future dental applications.
PB - Iop Publishing Ltd, Bristol
T2 - Journal of Physics D-Applied Physics
T1 - Effects of non-thermal atmospheric plasma on human periodontal ligament mesenchymal stem cells
IS - 34
VL - 46
DO - 10.1088/0022-3727/46/34/345401
ER -
@article{
author = "Miletić, Maja and Mojsilović, S. and Okić-Đorđević, Ivana and Maletić, Dejan and Puač, Nevena and Lazović, Saša and Malović, Gordana and Milenković, P. and Petrović, Zoran Lj. and Bugarski, Diana",
year = "2013",
abstract = "Here we investigate the influences of non-thermal atmospheric plasma on human mesenchymal stem cells isolated from periodontal ligament (hPDL-MSCs). A specially redesigned plasma needle was used as the source of low-temperature plasma and its effects on different hPDL-MSC functions were investigated. Cell cultures were obtained from extracted normal impacted third molars and characterized for their phenotype and multi-potential differentiation. The hPDL-MSCs possessed all the typical MSC properties, including clonogenic ability, high proliferation rate, specific phenotype and multilineage differentiation. The data regarding the interaction of plasma with hPDL-MSCs demonstrated that plasma treatment inhibited the migration of hPDL-MSCs and induced some detachment, while not affecting their viability. Additionally, plasma significantly attenuated hPDL-MSCs' proliferation, but promoted their osteogenic differentiation. The results of this study indicated that a non-thermal plasma offers specific activity with non-destructive properties that can be advantageous for future dental applications.",
publisher = "Iop Publishing Ltd, Bristol",
journal = "Journal of Physics D-Applied Physics",
title = "Effects of non-thermal atmospheric plasma on human periodontal ligament mesenchymal stem cells",
number = "34",
volume = "46",
doi = "10.1088/0022-3727/46/34/345401"
}
Miletić, M., Mojsilović, S., Okić-Đorđević, I., Maletić, D., Puač, N., Lazović, S., Malović, G., Milenković, P., Petrović, Z. Lj.,& Bugarski, D.. (2013). Effects of non-thermal atmospheric plasma on human periodontal ligament mesenchymal stem cells. in Journal of Physics D-Applied Physics
Iop Publishing Ltd, Bristol., 46(34).
https://doi.org/10.1088/0022-3727/46/34/345401
Miletić M, Mojsilović S, Okić-Đorđević I, Maletić D, Puač N, Lazović S, Malović G, Milenković P, Petrović ZL, Bugarski D. Effects of non-thermal atmospheric plasma on human periodontal ligament mesenchymal stem cells. in Journal of Physics D-Applied Physics. 2013;46(34).
doi:10.1088/0022-3727/46/34/345401 .
Miletić, Maja, Mojsilović, S., Okić-Đorđević, Ivana, Maletić, Dejan, Puač, Nevena, Lazović, Saša, Malović, Gordana, Milenković, P., Petrović, Zoran Lj., Bugarski, Diana, "Effects of non-thermal atmospheric plasma on human periodontal ligament mesenchymal stem cells" in Journal of Physics D-Applied Physics, 46, no. 34 (2013),
https://doi.org/10.1088/0022-3727/46/34/345401 . .
