Effects of non-thermal atmospheric plasma on human periodontal ligament mesenchymal stem cells
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Authors
Miletić, Maja
Mojsilović, S.
Okić-Đorđević, Ivana

Maletić, Dejan
Puač, Nevena

Lazović, Saša

Malović, Gordana

Milenković, P.
Petrović, Zoran Lj.

Bugarski, Diana

Article (Published version)

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Here we investigate the influences of non-thermal atmospheric plasma on human mesenchymal stem cells isolated from periodontal ligament (hPDL-MSCs). A specially redesigned plasma needle was used as the source of low-temperature plasma and its effects on different hPDL-MSC functions were investigated. Cell cultures were obtained from extracted normal impacted third molars and characterized for their phenotype and multi-potential differentiation. The hPDL-MSCs possessed all the typical MSC properties, including clonogenic ability, high proliferation rate, specific phenotype and multilineage differentiation. The data regarding the interaction of plasma with hPDL-MSCs demonstrated that plasma treatment inhibited the migration of hPDL-MSCs and induced some detachment, while not affecting their viability. Additionally, plasma significantly attenuated hPDL-MSCs' proliferation, but promoted their osteogenic differentiation. The results of this study indicated that a non-thermal plasma offers s...pecific activity with non-destructive properties that can be advantageous for future dental applications.
Source:
Journal of Physics D-Applied Physics, 2013, 46, 34Publisher:
- Iop Publishing Ltd, Bristol
Funding / projects:
- Regenerative and modulatory potential of adult stem cells (RS-175062)
- Fundamental processes and applications of particle transport in non-equilibrium plasmas, traps and nanostructures (RS-171037)
- Application of low temperature plasmas in biomedicine, environmental protection and nanotechnologies (RS-41011)
DOI: 10.1088/0022-3727/46/34/345401
ISSN: 0022-3727
WoS: 000323062400017
Scopus: 2-s2.0-84882360715
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Institut za medicinska istraživanjaTY - JOUR AU - Miletić, Maja AU - Mojsilović, S. AU - Okić-Đorđević, Ivana AU - Maletić, Dejan AU - Puač, Nevena AU - Lazović, Saša AU - Malović, Gordana AU - Milenković, P. AU - Petrović, Zoran Lj. AU - Bugarski, Diana PY - 2013 UR - http://rimi.imi.bg.ac.rs/handle/123456789/487 AB - Here we investigate the influences of non-thermal atmospheric plasma on human mesenchymal stem cells isolated from periodontal ligament (hPDL-MSCs). A specially redesigned plasma needle was used as the source of low-temperature plasma and its effects on different hPDL-MSC functions were investigated. Cell cultures were obtained from extracted normal impacted third molars and characterized for their phenotype and multi-potential differentiation. The hPDL-MSCs possessed all the typical MSC properties, including clonogenic ability, high proliferation rate, specific phenotype and multilineage differentiation. The data regarding the interaction of plasma with hPDL-MSCs demonstrated that plasma treatment inhibited the migration of hPDL-MSCs and induced some detachment, while not affecting their viability. Additionally, plasma significantly attenuated hPDL-MSCs' proliferation, but promoted their osteogenic differentiation. The results of this study indicated that a non-thermal plasma offers specific activity with non-destructive properties that can be advantageous for future dental applications. PB - Iop Publishing Ltd, Bristol T2 - Journal of Physics D-Applied Physics T1 - Effects of non-thermal atmospheric plasma on human periodontal ligament mesenchymal stem cells IS - 34 VL - 46 DO - 10.1088/0022-3727/46/34/345401 ER -
@article{ author = "Miletić, Maja and Mojsilović, S. and Okić-Đorđević, Ivana and Maletić, Dejan and Puač, Nevena and Lazović, Saša and Malović, Gordana and Milenković, P. and Petrović, Zoran Lj. and Bugarski, Diana", year = "2013", abstract = "Here we investigate the influences of non-thermal atmospheric plasma on human mesenchymal stem cells isolated from periodontal ligament (hPDL-MSCs). A specially redesigned plasma needle was used as the source of low-temperature plasma and its effects on different hPDL-MSC functions were investigated. Cell cultures were obtained from extracted normal impacted third molars and characterized for their phenotype and multi-potential differentiation. The hPDL-MSCs possessed all the typical MSC properties, including clonogenic ability, high proliferation rate, specific phenotype and multilineage differentiation. The data regarding the interaction of plasma with hPDL-MSCs demonstrated that plasma treatment inhibited the migration of hPDL-MSCs and induced some detachment, while not affecting their viability. Additionally, plasma significantly attenuated hPDL-MSCs' proliferation, but promoted their osteogenic differentiation. The results of this study indicated that a non-thermal plasma offers specific activity with non-destructive properties that can be advantageous for future dental applications.", publisher = "Iop Publishing Ltd, Bristol", journal = "Journal of Physics D-Applied Physics", title = "Effects of non-thermal atmospheric plasma on human periodontal ligament mesenchymal stem cells", number = "34", volume = "46", doi = "10.1088/0022-3727/46/34/345401" }
Miletić, M., Mojsilović, S., Okić-Đorđević, I., Maletić, D., Puač, N., Lazović, S., Malović, G., Milenković, P., Petrović, Z. Lj.,& Bugarski, D.. (2013). Effects of non-thermal atmospheric plasma on human periodontal ligament mesenchymal stem cells. in Journal of Physics D-Applied Physics Iop Publishing Ltd, Bristol., 46(34). https://doi.org/10.1088/0022-3727/46/34/345401
Miletić M, Mojsilović S, Okić-Đorđević I, Maletić D, Puač N, Lazović S, Malović G, Milenković P, Petrović ZL, Bugarski D. Effects of non-thermal atmospheric plasma on human periodontal ligament mesenchymal stem cells. in Journal of Physics D-Applied Physics. 2013;46(34). doi:10.1088/0022-3727/46/34/345401 .
Miletić, Maja, Mojsilović, S., Okić-Đorđević, Ivana, Maletić, Dejan, Puač, Nevena, Lazović, Saša, Malović, Gordana, Milenković, P., Petrović, Zoran Lj., Bugarski, Diana, "Effects of non-thermal atmospheric plasma on human periodontal ligament mesenchymal stem cells" in Journal of Physics D-Applied Physics, 46, no. 34 (2013), https://doi.org/10.1088/0022-3727/46/34/345401 . .