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dc.creatorKrstić, Jelena
dc.creatorJauković, Aleksandra
dc.creatorMojsilović, Slavko
dc.creatorOkić Đorđević, Ivana
dc.creatorTrivanović, Drenka
dc.creatorIlić, Vesna
dc.creatorSantibanez, Juan F.
dc.creatorBugarski, Diana
dc.date.accessioned2021-04-20T12:33:45Z
dc.date.available2021-04-20T12:33:45Z
dc.date.issued2013
dc.identifier.issn1065-6995
dc.identifier.urihttp://rimi.imi.bg.ac.rs/handle/123456789/484
dc.description.abstractThe aim of this study has been to elucidate how different oxygen levels impact the effects of Interleukin-17 (IL-17) on angiogenic properties of endothelial cells. Two endothelial cell lines, mouse MS-1 and human EA.hy 926, were grown in 20% and 3% O-2 and their angiogenic abilities analyzed after IL-17 treatment: proliferation, apoptosis, migration and tubulogenesis. Expression of endothelial nitric oxide synthase (eNOS) and cyclooxygenase-2 (Cox-2) was also measured. Considering EA.hy 926 cell line, hypoxia alone reduced proliferation, survival and migration, but not their ability to form tubules. When cultured at 20% O-2, IL-17 stimulated proliferation, migration and tubulogenesis, whereas a hypoxic environment did not affect their migration and proliferation, but increased their survival and tubulogenic properties. Expression of eNOS and Cox-2 increased by both IL-17 and hypoxia, as well as with their combination. With the MS-1 cell line hypoxia did not affect proliferation, survival, migration and tubule formation. At 20% O-2, IL-17 did not alter their proliferation,but inhibited migration and stimulated tubule formation. At 3% O-2, only the stimulating effect of IL-17 on tubulogenesis was evident. The constitutive expression of eNOS was unaffected by oxygen concentrations or IL-17 supplementation, whereas both IL-17 and hypoxia upregulated Cox-2 expression. Thus the effects of IL-17 on the angiogenic properties of endothelial cells depend on both the cell line used and the oxygen concentration.en
dc.publisherWiley, Hoboken
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175062/RS//
dc.rightsrestrictedAccess
dc.sourceCell Biology International
dc.subjectangiogenesisen
dc.subjectendothelial cellsen
dc.subjectEAen
dc.subjecthy 926en
dc.subjecthypoxiaen
dc.subjectIL-17en
dc.subjectMS-1en
dc.titleIn vitro effects of IL-17 on angiogenic properties of endothelial cells in relation to oxygen levelsen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage1170
dc.citation.issue11
dc.citation.other37(11): 1162-1170
dc.citation.rankM23
dc.citation.spage1162
dc.citation.volume37
dc.identifier.doi10.1002/cbin.10144
dc.identifier.pmid23765637
dc.identifier.scopus2-s2.0-84885868031
dc.identifier.wos000325489500004
dc.type.versionpublishedVersion


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