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In vitro effects of IL-17 on angiogenic properties of endothelial cells in relation to oxygen levels
dc.creator | Krstić, Jelena | |
dc.creator | Jauković, Aleksandra | |
dc.creator | Mojsilović, Slavko | |
dc.creator | Okić Đorđević, Ivana | |
dc.creator | Trivanović, Drenka | |
dc.creator | Ilić, Vesna | |
dc.creator | Santibanez, Juan F. | |
dc.creator | Bugarski, Diana | |
dc.date.accessioned | 2021-04-20T12:33:45Z | |
dc.date.available | 2021-04-20T12:33:45Z | |
dc.date.issued | 2013 | |
dc.identifier.issn | 1065-6995 | |
dc.identifier.uri | http://rimi.imi.bg.ac.rs/handle/123456789/484 | |
dc.description.abstract | The aim of this study has been to elucidate how different oxygen levels impact the effects of Interleukin-17 (IL-17) on angiogenic properties of endothelial cells. Two endothelial cell lines, mouse MS-1 and human EA.hy 926, were grown in 20% and 3% O-2 and their angiogenic abilities analyzed after IL-17 treatment: proliferation, apoptosis, migration and tubulogenesis. Expression of endothelial nitric oxide synthase (eNOS) and cyclooxygenase-2 (Cox-2) was also measured. Considering EA.hy 926 cell line, hypoxia alone reduced proliferation, survival and migration, but not their ability to form tubules. When cultured at 20% O-2, IL-17 stimulated proliferation, migration and tubulogenesis, whereas a hypoxic environment did not affect their migration and proliferation, but increased their survival and tubulogenic properties. Expression of eNOS and Cox-2 increased by both IL-17 and hypoxia, as well as with their combination. With the MS-1 cell line hypoxia did not affect proliferation, survival, migration and tubule formation. At 20% O-2, IL-17 did not alter their proliferation,but inhibited migration and stimulated tubule formation. At 3% O-2, only the stimulating effect of IL-17 on tubulogenesis was evident. The constitutive expression of eNOS was unaffected by oxygen concentrations or IL-17 supplementation, whereas both IL-17 and hypoxia upregulated Cox-2 expression. Thus the effects of IL-17 on the angiogenic properties of endothelial cells depend on both the cell line used and the oxygen concentration. | en |
dc.publisher | Wiley, Hoboken | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175062/RS// | |
dc.rights | restrictedAccess | |
dc.source | Cell Biology International | |
dc.subject | angiogenesis | en |
dc.subject | endothelial cells | en |
dc.subject | EA | en |
dc.subject | hy 926 | en |
dc.subject | hypoxia | en |
dc.subject | IL-17 | en |
dc.subject | MS-1 | en |
dc.title | In vitro effects of IL-17 on angiogenic properties of endothelial cells in relation to oxygen levels | en |
dc.type | article | |
dc.rights.license | ARR | |
dc.citation.epage | 1170 | |
dc.citation.issue | 11 | |
dc.citation.other | 37(11): 1162-1170 | |
dc.citation.rank | M23 | |
dc.citation.spage | 1162 | |
dc.citation.volume | 37 | |
dc.identifier.doi | 10.1002/cbin.10144 | |
dc.identifier.pmid | 23765637 | |
dc.identifier.scopus | 2-s2.0-84885868031 | |
dc.identifier.wos | 000325489500004 | |
dc.type.version | publishedVersion |