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dc.creatorLagundzin, Dragana
dc.creatorVučić, Vesna M.
dc.creatorGlibetić, Marija
dc.creatorNedić, Olgica
dc.date.accessioned2021-04-20T12:33:37Z
dc.date.available2021-04-20T12:33:37Z
dc.date.issued2013
dc.identifier.issn1526-484X
dc.identifier.urihttp://rimi.imi.bg.ac.rs/handle/123456789/482
dc.description.abstractPhysical activity is accompanied by the changes in Insulin-like Growth Factor I (IGF-I)/IGF-Binding Protein 1 (IGFBP-1) axis. Inconsistent results concerning IGF-I and IGFBP-1 levels were reported. In this study we have raised some questions on the events that occur at the molecular level of the exercise-related IGFBP-1 changes. We have examined the fragmentation pattern of IGFBP-1, IGFBP-1 protease activity, interaction between IGFBP-1 and alpha2-macroglobulin (alpha 2M), and possible existence of minor structural changes of IGFBP-1 in professional soccer players. Athletes had significantly greater amounts of fragmented IGFBP-1, whereas no difference was found in the amount of intact IGFBP-1 compared with controls. An increased activity of matrix metalloprotease-9 (MMP-9) was detected in athletes, causing IGFBP-1 degradation down to the fragment of 9 kDa as the major one. The amount of alpha 2M, which protects IGFBP-1 from proteolysis, or the amount of IGFBP-1/alpha 2M complexes was unaltered. Finally, we have examined whether IGFBP-1 isolated from soccer players exhibited altered reactivity with several chemical surfaces used in surface-enhanced laser desorption/ionization-time of flight mass spectrometry (SELDI-TOF MS). Different reactivity was detected with anion and cation exchangers, suggesting existence of at least one sequence within IGFBP-1, whose ionization pattern was not equal in athletes and controls. Differences in spectra obtained with ion exchanges may reflect differences in IGFBP-1 phosphorylation. Physiological implications of the events described in this study on the IGF-I availability are, at this time, unknown. It can be hypothesized that IGFBP-1 proteolysis leads to altered distribution of IGF-I among IGFBPs, which may affect the final IGF-associated response.en
dc.publisherHuman Kinetics Publ Inc, Champaign
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173042/RS//
dc.rightsrestrictedAccess
dc.sourceInternational Journal of Sport Nutrition & Exercise Metabolism
dc.subjectIGFBP-1en
dc.subjectsoccer playersen
dc.subjectproteolysisen
dc.subjectSELDI-TOF MSen
dc.titleAlteration of IGFBP-1 in Soccer Players Due to Intensive Trainingen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage457
dc.citation.issue5
dc.citation.other23(5): 449-457
dc.citation.rankM22
dc.citation.spage449
dc.citation.volume23
dc.identifier.doi10.1123/ijsnem.23.5.449
dc.identifier.pmid23535908
dc.identifier.scopus2-s2.0-84888807209
dc.identifier.wos000327285200004
dc.type.versionpublishedVersion


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