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Alteration of IGFBP-1 in Soccer Players Due to Intensive Training
dc.creator | Lagundzin, Dragana | |
dc.creator | Vučić, Vesna M. | |
dc.creator | Glibetić, Marija | |
dc.creator | Nedić, Olgica | |
dc.date.accessioned | 2021-04-20T12:33:37Z | |
dc.date.available | 2021-04-20T12:33:37Z | |
dc.date.issued | 2013 | |
dc.identifier.issn | 1526-484X | |
dc.identifier.uri | http://rimi.imi.bg.ac.rs/handle/123456789/482 | |
dc.description.abstract | Physical activity is accompanied by the changes in Insulin-like Growth Factor I (IGF-I)/IGF-Binding Protein 1 (IGFBP-1) axis. Inconsistent results concerning IGF-I and IGFBP-1 levels were reported. In this study we have raised some questions on the events that occur at the molecular level of the exercise-related IGFBP-1 changes. We have examined the fragmentation pattern of IGFBP-1, IGFBP-1 protease activity, interaction between IGFBP-1 and alpha2-macroglobulin (alpha 2M), and possible existence of minor structural changes of IGFBP-1 in professional soccer players. Athletes had significantly greater amounts of fragmented IGFBP-1, whereas no difference was found in the amount of intact IGFBP-1 compared with controls. An increased activity of matrix metalloprotease-9 (MMP-9) was detected in athletes, causing IGFBP-1 degradation down to the fragment of 9 kDa as the major one. The amount of alpha 2M, which protects IGFBP-1 from proteolysis, or the amount of IGFBP-1/alpha 2M complexes was unaltered. Finally, we have examined whether IGFBP-1 isolated from soccer players exhibited altered reactivity with several chemical surfaces used in surface-enhanced laser desorption/ionization-time of flight mass spectrometry (SELDI-TOF MS). Different reactivity was detected with anion and cation exchangers, suggesting existence of at least one sequence within IGFBP-1, whose ionization pattern was not equal in athletes and controls. Differences in spectra obtained with ion exchanges may reflect differences in IGFBP-1 phosphorylation. Physiological implications of the events described in this study on the IGF-I availability are, at this time, unknown. It can be hypothesized that IGFBP-1 proteolysis leads to altered distribution of IGF-I among IGFBPs, which may affect the final IGF-associated response. | en |
dc.publisher | Human Kinetics Publ Inc, Champaign | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173042/RS// | |
dc.rights | restrictedAccess | |
dc.source | International Journal of Sport Nutrition & Exercise Metabolism | |
dc.subject | IGFBP-1 | en |
dc.subject | soccer players | en |
dc.subject | proteolysis | en |
dc.subject | SELDI-TOF MS | en |
dc.title | Alteration of IGFBP-1 in Soccer Players Due to Intensive Training | en |
dc.type | article | |
dc.rights.license | ARR | |
dc.citation.epage | 457 | |
dc.citation.issue | 5 | |
dc.citation.other | 23(5): 449-457 | |
dc.citation.rank | M22 | |
dc.citation.spage | 449 | |
dc.citation.volume | 23 | |
dc.identifier.doi | 10.1123/ijsnem.23.5.449 | |
dc.identifier.pmid | 23535908 | |
dc.identifier.scopus | 2-s2.0-84888807209 | |
dc.identifier.wos | 000327285200004 | |
dc.type.version | publishedVersion |