We studied TOP2A amplification (using FISH methods), and TOP2A and BRCA1 protein overexpression (immunohistochemistry) in four molecular subtypes of breast cancer. Of 53 patients, 32 showed TOP2A and 38 showed BRCA1 overexpression. The highest percentage of TOP2A amplification (47.8%) and deletion (13%) was detected in Luminal B subtypes. Of 11 Luminal B tumors with TOP2A amplification, 9 (81.8%) overexpressed TOP2A. BRCA1 protein overexpression showed significant positive correlation with TOP2A protein expression. BRCA1 and TOP2A proteins exhibited similar patterns of expression in Luminal B and triple-negative breast cancer, suggesting the same prognosis in those patients.
Keywords:
BRCA1 / TOP2A / FISH / immunohistochemistry / molecular subtypes of breast cancer
Source:
Archives of Biological Sciences, 2013, 65, 2, 511-518
TY - JOUR
AU - Mitrović, Olivera
AU - Mićić, Mileva
AU - Čokić, Vladan
AU - Koko, Vesna
AU - Đikić, Dragoslava
AU - Budeč, Mirela
AU - Vignjević, Sanja
AU - Breković, Tijana
PY - 2013
UR - http://rimi.imi.bg.ac.rs/handle/123456789/465
AB - We studied TOP2A amplification (using FISH methods), and TOP2A and BRCA1 protein overexpression (immunohistochemistry) in four molecular subtypes of breast cancer. Of 53 patients, 32 showed TOP2A and 38 showed BRCA1 overexpression. The highest percentage of TOP2A amplification (47.8%) and deletion (13%) was detected in Luminal B subtypes. Of 11 Luminal B tumors with TOP2A amplification, 9 (81.8%) overexpressed TOP2A. BRCA1 protein overexpression showed significant positive correlation with TOP2A protein expression. BRCA1 and TOP2A proteins exhibited similar patterns of expression in Luminal B and triple-negative breast cancer, suggesting the same prognosis in those patients.
PB - Srpsko biološko društvo, Beograd, i dr.
T2 - Archives of Biological Sciences
T1 - BRCA1 and TOP2A gene amplification and protein expression in four molecular subtypes of breast cancer
EP - 518
IS - 2
SP - 511
VL - 65
DO - 10.2298/ABS1302511M
UR - conv_2964
ER -
@article{
author = "Mitrović, Olivera and Mićić, Mileva and Čokić, Vladan and Koko, Vesna and Đikić, Dragoslava and Budeč, Mirela and Vignjević, Sanja and Breković, Tijana",
year = "2013",
abstract = "We studied TOP2A amplification (using FISH methods), and TOP2A and BRCA1 protein overexpression (immunohistochemistry) in four molecular subtypes of breast cancer. Of 53 patients, 32 showed TOP2A and 38 showed BRCA1 overexpression. The highest percentage of TOP2A amplification (47.8%) and deletion (13%) was detected in Luminal B subtypes. Of 11 Luminal B tumors with TOP2A amplification, 9 (81.8%) overexpressed TOP2A. BRCA1 protein overexpression showed significant positive correlation with TOP2A protein expression. BRCA1 and TOP2A proteins exhibited similar patterns of expression in Luminal B and triple-negative breast cancer, suggesting the same prognosis in those patients.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "BRCA1 and TOP2A gene amplification and protein expression in four molecular subtypes of breast cancer",
pages = "518-511",
number = "2",
volume = "65",
doi = "10.2298/ABS1302511M",
url = "conv_2964"
}
Mitrović, O., Mićić, M., Čokić, V., Koko, V., Đikić, D., Budeč, M., Vignjević, S.,& Breković, T.. (2013). BRCA1 and TOP2A gene amplification and protein expression in four molecular subtypes of breast cancer. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 65(2), 511-518.
https://doi.org/10.2298/ABS1302511M
conv_2964
Mitrović O, Mićić M, Čokić V, Koko V, Đikić D, Budeč M, Vignjević S, Breković T. BRCA1 and TOP2A gene amplification and protein expression in four molecular subtypes of breast cancer. in Archives of Biological Sciences. 2013;65(2):511-518.
doi:10.2298/ABS1302511M
conv_2964 .
Mitrović, Olivera, Mićić, Mileva, Čokić, Vladan, Koko, Vesna, Đikić, Dragoslava, Budeč, Mirela, Vignjević, Sanja, Breković, Tijana, "BRCA1 and TOP2A gene amplification and protein expression in four molecular subtypes of breast cancer" in Archives of Biological Sciences, 65, no. 2 (2013):511-518,
https://doi.org/10.2298/ABS1302511M .,
conv_2964 .
