Serum amyloid A isoforms in serum and milk from cows with Staphylococcus aureus subclinical mastitis
Samo za registrovane korisnike
2012
Autori
Kovačević-Filipović, MilicaIlić, Vesna
Vujcić, Zoran
Dojnov, Biljana
Stevanov-Pavlović, Marija
Mijacević, Zora
Božić, Tatjana
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Serum amyloid A proteins (SAA) are very sensitive acute phase proteins, displaying multiple isoforms in plasma and different body fluids. They are currently under investigation as biomarkers of diseases. The aim of the present study was to compare the concentration and isoform expression of SAA in serum and milk of cows with bacteriologically negative milk (control group) and naturally occurring Staphylococcus aureus (S. aureus) subclinical mastitis (subclinical mastitis group). Somatic cell count (SCC) and bacteriological analyses were performed to establish the control and subclinical mastitis group. SAA concentration was evaluated using a commercial ELISA kit, while expression of different isoforms (serum A-SAA and milk M-SAA3 isoforms) was visualized by denaturing isoelectrical focusing and immunoblotting. The SAA concentrations in sera and milk of cows in the subclinical mastitis group were three and 100 times higher than in those from the control group of cows, respectively. Cows... in the subclinical mastitis group had more acidic SAA isoforms in serum with the most prominent one at pI 5.5. This isoform was not detected in sera from the control group. Milk samples in the subclinical mastitis group contained abundant highly alkaline M-SAA3 isoforms and most of the serum isoforms, except for that at pI 5.5. In the subclinical mastitis group SAA isoforms with equivalent pI as serum isoforms accounted for 20% of the total SAA concentration in milk. There were significant differences in the concentrations and isoform patterns of SAA in serum and milk between the control and subclinical mastitis groups of cows. Also, we demonstrated that serum SAA isoforms were not transferred to milk proportion to their plasma content.
Ključne reči:
Serum amyloid A / Isoforms / Subclinical mastitis / Staphylococcus aureusIzvor:
Veterinary Immunology & Immunopathology, 2012, 145, 1-2, 120-128Izdavač:
- Elsevier, Amsterdam
Finansiranje / projekti:
- Antioksidativna zaštita i potencijali za diferencijaciju i regeneraciju mezenhimalnih matičnih ćelija iz različitih tkiva tokom procesa starenja (RS-175061)
- Regenerativni i modulatorni potencijal adultnih matičnih ćelija (RS-175062)
- Proizvodnja, izolovanje i karakterizacija enzima i malih molekula i njihova primena u rastvornom i imobilizovanom obliku u biotehnologiji hrane, biogorivima i zaštititi životne sredine (RS-172048)
- Unapređenje i razvoj higijenskih i tehnoloških postupaka u proizvodnji namirnica životinjskog porekla u cilju dobijanja kvalitetnih i bezbednih proizvoda konkurentnih na svetskom tržištu (RS-46009)
DOI: 10.1016/j.vetimm.2011.10.015
ISSN: 0165-2427
PubMed: 22100192
WoS: 000301159200013
Scopus: 2-s2.0-84856555615
Institucija/grupa
Institut za medicinska istraživanjaTY - JOUR AU - Kovačević-Filipović, Milica AU - Ilić, Vesna AU - Vujcić, Zoran AU - Dojnov, Biljana AU - Stevanov-Pavlović, Marija AU - Mijacević, Zora AU - Božić, Tatjana PY - 2012 UR - http://rimi.imi.bg.ac.rs/handle/123456789/447 AB - Serum amyloid A proteins (SAA) are very sensitive acute phase proteins, displaying multiple isoforms in plasma and different body fluids. They are currently under investigation as biomarkers of diseases. The aim of the present study was to compare the concentration and isoform expression of SAA in serum and milk of cows with bacteriologically negative milk (control group) and naturally occurring Staphylococcus aureus (S. aureus) subclinical mastitis (subclinical mastitis group). Somatic cell count (SCC) and bacteriological analyses were performed to establish the control and subclinical mastitis group. SAA concentration was evaluated using a commercial ELISA kit, while expression of different isoforms (serum A-SAA and milk M-SAA3 isoforms) was visualized by denaturing isoelectrical focusing and immunoblotting. The SAA concentrations in sera and milk of cows in the subclinical mastitis group were three and 100 times higher than in those from the control group of cows, respectively. Cows in the subclinical mastitis group had more acidic SAA isoforms in serum with the most prominent one at pI 5.5. This isoform was not detected in sera from the control group. Milk samples in the subclinical mastitis group contained abundant highly alkaline M-SAA3 isoforms and most of the serum isoforms, except for that at pI 5.5. In the subclinical mastitis group SAA isoforms with equivalent pI as serum isoforms accounted for 20% of the total SAA concentration in milk. There were significant differences in the concentrations and isoform patterns of SAA in serum and milk between the control and subclinical mastitis groups of cows. Also, we demonstrated that serum SAA isoforms were not transferred to milk proportion to their plasma content. PB - Elsevier, Amsterdam T2 - Veterinary Immunology & Immunopathology T1 - Serum amyloid A isoforms in serum and milk from cows with Staphylococcus aureus subclinical mastitis EP - 128 IS - 1-2 SP - 120 VL - 145 DO - 10.1016/j.vetimm.2011.10.015 ER -
@article{ author = "Kovačević-Filipović, Milica and Ilić, Vesna and Vujcić, Zoran and Dojnov, Biljana and Stevanov-Pavlović, Marija and Mijacević, Zora and Božić, Tatjana", year = "2012", abstract = "Serum amyloid A proteins (SAA) are very sensitive acute phase proteins, displaying multiple isoforms in plasma and different body fluids. They are currently under investigation as biomarkers of diseases. The aim of the present study was to compare the concentration and isoform expression of SAA in serum and milk of cows with bacteriologically negative milk (control group) and naturally occurring Staphylococcus aureus (S. aureus) subclinical mastitis (subclinical mastitis group). Somatic cell count (SCC) and bacteriological analyses were performed to establish the control and subclinical mastitis group. SAA concentration was evaluated using a commercial ELISA kit, while expression of different isoforms (serum A-SAA and milk M-SAA3 isoforms) was visualized by denaturing isoelectrical focusing and immunoblotting. The SAA concentrations in sera and milk of cows in the subclinical mastitis group were three and 100 times higher than in those from the control group of cows, respectively. Cows in the subclinical mastitis group had more acidic SAA isoforms in serum with the most prominent one at pI 5.5. This isoform was not detected in sera from the control group. Milk samples in the subclinical mastitis group contained abundant highly alkaline M-SAA3 isoforms and most of the serum isoforms, except for that at pI 5.5. In the subclinical mastitis group SAA isoforms with equivalent pI as serum isoforms accounted for 20% of the total SAA concentration in milk. There were significant differences in the concentrations and isoform patterns of SAA in serum and milk between the control and subclinical mastitis groups of cows. Also, we demonstrated that serum SAA isoforms were not transferred to milk proportion to their plasma content.", publisher = "Elsevier, Amsterdam", journal = "Veterinary Immunology & Immunopathology", title = "Serum amyloid A isoforms in serum and milk from cows with Staphylococcus aureus subclinical mastitis", pages = "128-120", number = "1-2", volume = "145", doi = "10.1016/j.vetimm.2011.10.015" }
Kovačević-Filipović, M., Ilić, V., Vujcić, Z., Dojnov, B., Stevanov-Pavlović, M., Mijacević, Z.,& Božić, T.. (2012). Serum amyloid A isoforms in serum and milk from cows with Staphylococcus aureus subclinical mastitis. in Veterinary Immunology & Immunopathology Elsevier, Amsterdam., 145(1-2), 120-128. https://doi.org/10.1016/j.vetimm.2011.10.015
Kovačević-Filipović M, Ilić V, Vujcić Z, Dojnov B, Stevanov-Pavlović M, Mijacević Z, Božić T. Serum amyloid A isoforms in serum and milk from cows with Staphylococcus aureus subclinical mastitis. in Veterinary Immunology & Immunopathology. 2012;145(1-2):120-128. doi:10.1016/j.vetimm.2011.10.015 .
Kovačević-Filipović, Milica, Ilić, Vesna, Vujcić, Zoran, Dojnov, Biljana, Stevanov-Pavlović, Marija, Mijacević, Zora, Božić, Tatjana, "Serum amyloid A isoforms in serum and milk from cows with Staphylococcus aureus subclinical mastitis" in Veterinary Immunology & Immunopathology, 145, no. 1-2 (2012):120-128, https://doi.org/10.1016/j.vetimm.2011.10.015 . .