Kinetics of parasite burdens in blood and tissues during murine toxoplasmosis
Samo za registrovane korisnike
2012
Autori
Đurković-Đaković, Olgica
Đokić, Vitomir

Vujanić, Marija

Živković, Tijana

Bobić, Branko

Nikolić, Aleksandra
Slavić, Ksenija

Klun, Ivana

Ivović, Vladimir
Članak u časopisu (Objavljena verzija)

Metapodaci
Prikaz svih podataka o dokumentuApstrakt
A sensitive real-time PCR technique was used to examine the distribution of Toxoplasma gondii in the blood and tissues of mice during acute and chronic infection. Groups of Swiss Albino mice, inoculated i.p. with 10(2) or 10(6) tachyzoites of the RH strain as a typical type-1 strain, or fed 10 cysts of the Me49 strain as a typical type-2 strain, were killed at different time points post-infection (p.i.), and blood and organs including the lungs, brain and liver were harvested for DNA extraction. Toxoplasma DNA was quantified by a real-time PCR targeted at the 529 bp gene fragment, with a detection limit of a single parasite per g/ml of tissue. The results showed a strain- and dose-dependent spread of Toxoplasma. In infection with type-1 parasites, in case of a high infective dose, Toxoplasma DNA was detected within 24 h p.i. in all analyzed tissues including the brain. Conversely, in case of a low infective dose, parasitaemia was undetectable early p.i., at a time when Toxoplasma DNA w...as detected in the tissues, but reached very high levels as infection progressed. With both infective doses, pre-death parasite burdens were higher in the blood than in the tissues, whereas the same loads in the lungs suggest that reaching these Toxoplasma burdens may be critical for survival. In infection with Me49 parasites, steady high parasite burdens were noted up to the end of the experiment at d42 only in the brain, parasitaemia was low but detectable throughout, and Toxoplasma DNA was completely cleared only from the liver. These data are important to better understand the pathogenesis of toxoplasmosis, and also as baseline data for the experimental evaluation of novel chemotherapeutics.
Ključne reči:
Toxoplasma gondii / Murine infection / Parasite burdens / Parasitaemia / Real-time PCRIzvor:
Experimental Parasitology, 2012, 131, 3, 372-376Izdavač:
- Academic Press Inc Elsevier Science, San Diego
Finansiranje / projekti:
- Kontrola infekcija apikompleksnim patogenima: od novih mesta delovanja leka do predikcije (RS-41019)
DOI: 10.1016/j.exppara.2012.05.006
ISSN: 0014-4894
PubMed: 22613495
WoS: 000306158100016
Scopus: 2-s2.0-84862586645
Institucija/grupa
Institut za medicinska istraživanjaTY - JOUR AU - Đurković-Đaković, Olgica AU - Đokić, Vitomir AU - Vujanić, Marija AU - Živković, Tijana AU - Bobić, Branko AU - Nikolić, Aleksandra AU - Slavić, Ksenija AU - Klun, Ivana AU - Ivović, Vladimir PY - 2012 UR - http://rimi.imi.bg.ac.rs/handle/123456789/418 AB - A sensitive real-time PCR technique was used to examine the distribution of Toxoplasma gondii in the blood and tissues of mice during acute and chronic infection. Groups of Swiss Albino mice, inoculated i.p. with 10(2) or 10(6) tachyzoites of the RH strain as a typical type-1 strain, or fed 10 cysts of the Me49 strain as a typical type-2 strain, were killed at different time points post-infection (p.i.), and blood and organs including the lungs, brain and liver were harvested for DNA extraction. Toxoplasma DNA was quantified by a real-time PCR targeted at the 529 bp gene fragment, with a detection limit of a single parasite per g/ml of tissue. The results showed a strain- and dose-dependent spread of Toxoplasma. In infection with type-1 parasites, in case of a high infective dose, Toxoplasma DNA was detected within 24 h p.i. in all analyzed tissues including the brain. Conversely, in case of a low infective dose, parasitaemia was undetectable early p.i., at a time when Toxoplasma DNA was detected in the tissues, but reached very high levels as infection progressed. With both infective doses, pre-death parasite burdens were higher in the blood than in the tissues, whereas the same loads in the lungs suggest that reaching these Toxoplasma burdens may be critical for survival. In infection with Me49 parasites, steady high parasite burdens were noted up to the end of the experiment at d42 only in the brain, parasitaemia was low but detectable throughout, and Toxoplasma DNA was completely cleared only from the liver. These data are important to better understand the pathogenesis of toxoplasmosis, and also as baseline data for the experimental evaluation of novel chemotherapeutics. PB - Academic Press Inc Elsevier Science, San Diego T2 - Experimental Parasitology T1 - Kinetics of parasite burdens in blood and tissues during murine toxoplasmosis EP - 376 IS - 3 SP - 372 VL - 131 DO - 10.1016/j.exppara.2012.05.006 ER -
@article{ author = "Đurković-Đaković, Olgica and Đokić, Vitomir and Vujanić, Marija and Živković, Tijana and Bobić, Branko and Nikolić, Aleksandra and Slavić, Ksenija and Klun, Ivana and Ivović, Vladimir", year = "2012", abstract = "A sensitive real-time PCR technique was used to examine the distribution of Toxoplasma gondii in the blood and tissues of mice during acute and chronic infection. Groups of Swiss Albino mice, inoculated i.p. with 10(2) or 10(6) tachyzoites of the RH strain as a typical type-1 strain, or fed 10 cysts of the Me49 strain as a typical type-2 strain, were killed at different time points post-infection (p.i.), and blood and organs including the lungs, brain and liver were harvested for DNA extraction. Toxoplasma DNA was quantified by a real-time PCR targeted at the 529 bp gene fragment, with a detection limit of a single parasite per g/ml of tissue. The results showed a strain- and dose-dependent spread of Toxoplasma. In infection with type-1 parasites, in case of a high infective dose, Toxoplasma DNA was detected within 24 h p.i. in all analyzed tissues including the brain. Conversely, in case of a low infective dose, parasitaemia was undetectable early p.i., at a time when Toxoplasma DNA was detected in the tissues, but reached very high levels as infection progressed. With both infective doses, pre-death parasite burdens were higher in the blood than in the tissues, whereas the same loads in the lungs suggest that reaching these Toxoplasma burdens may be critical for survival. In infection with Me49 parasites, steady high parasite burdens were noted up to the end of the experiment at d42 only in the brain, parasitaemia was low but detectable throughout, and Toxoplasma DNA was completely cleared only from the liver. These data are important to better understand the pathogenesis of toxoplasmosis, and also as baseline data for the experimental evaluation of novel chemotherapeutics.", publisher = "Academic Press Inc Elsevier Science, San Diego", journal = "Experimental Parasitology", title = "Kinetics of parasite burdens in blood and tissues during murine toxoplasmosis", pages = "376-372", number = "3", volume = "131", doi = "10.1016/j.exppara.2012.05.006" }
Đurković-Đaković, O., Đokić, V., Vujanić, M., Živković, T., Bobić, B., Nikolić, A., Slavić, K., Klun, I.,& Ivović, V.. (2012). Kinetics of parasite burdens in blood and tissues during murine toxoplasmosis. in Experimental Parasitology Academic Press Inc Elsevier Science, San Diego., 131(3), 372-376. https://doi.org/10.1016/j.exppara.2012.05.006
Đurković-Đaković O, Đokić V, Vujanić M, Živković T, Bobić B, Nikolić A, Slavić K, Klun I, Ivović V. Kinetics of parasite burdens in blood and tissues during murine toxoplasmosis. in Experimental Parasitology. 2012;131(3):372-376. doi:10.1016/j.exppara.2012.05.006 .
Đurković-Đaković, Olgica, Đokić, Vitomir, Vujanić, Marija, Živković, Tijana, Bobić, Branko, Nikolić, Aleksandra, Slavić, Ksenija, Klun, Ivana, Ivović, Vladimir, "Kinetics of parasite burdens in blood and tissues during murine toxoplasmosis" in Experimental Parasitology, 131, no. 3 (2012):372-376, https://doi.org/10.1016/j.exppara.2012.05.006 . .