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Kinetics of parasite burdens in blood and tissues during murine toxoplasmosis

Authorized Users Only
2012
Authors
Đurković-Đaković, Olgica
Đokić, Vitomir
Vujanić, Marija
Živković, Tijana
Bobić, Branko
Nikolić, Aleksandra
Slavić, Ksenija
Klun, Ivana
Ivović, Vladimir
Article (Published version)
Metadata
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Abstract
A sensitive real-time PCR technique was used to examine the distribution of Toxoplasma gondii in the blood and tissues of mice during acute and chronic infection. Groups of Swiss Albino mice, inoculated i.p. with 10(2) or 10(6) tachyzoites of the RH strain as a typical type-1 strain, or fed 10 cysts of the Me49 strain as a typical type-2 strain, were killed at different time points post-infection (p.i.), and blood and organs including the lungs, brain and liver were harvested for DNA extraction. Toxoplasma DNA was quantified by a real-time PCR targeted at the 529 bp gene fragment, with a detection limit of a single parasite per g/ml of tissue. The results showed a strain- and dose-dependent spread of Toxoplasma. In infection with type-1 parasites, in case of a high infective dose, Toxoplasma DNA was detected within 24 h p.i. in all analyzed tissues including the brain. Conversely, in case of a low infective dose, parasitaemia was undetectable early p.i., at a time when Toxoplasma DNA w...as detected in the tissues, but reached very high levels as infection progressed. With both infective doses, pre-death parasite burdens were higher in the blood than in the tissues, whereas the same loads in the lungs suggest that reaching these Toxoplasma burdens may be critical for survival. In infection with Me49 parasites, steady high parasite burdens were noted up to the end of the experiment at d42 only in the brain, parasitaemia was low but detectable throughout, and Toxoplasma DNA was completely cleared only from the liver. These data are important to better understand the pathogenesis of toxoplasmosis, and also as baseline data for the experimental evaluation of novel chemotherapeutics.

Keywords:
Toxoplasma gondii / Murine infection / Parasite burdens / Parasitaemia / Real-time PCR
Source:
Experimental Parasitology, 2012, 131, 3, 372-376
Publisher:
  • Academic Press Inc Elsevier Science, San Diego
Funding / projects:
  • Control of infections by Apicomplexan pathogens: from novel drug targets to prediction (RS-41019)

DOI: 10.1016/j.exppara.2012.05.006

ISSN: 0014-4894

PubMed: 22613495

WoS: 000306158100016

Scopus: 2-s2.0-84862586645
[ Google Scholar ]
30
28
URI
http://rimi.imi.bg.ac.rs/handle/123456789/418
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
Institut za medicinska istraživanja
TY  - JOUR
AU  - Đurković-Đaković, Olgica
AU  - Đokić, Vitomir
AU  - Vujanić, Marija
AU  - Živković, Tijana
AU  - Bobić, Branko
AU  - Nikolić, Aleksandra
AU  - Slavić, Ksenija
AU  - Klun, Ivana
AU  - Ivović, Vladimir
PY  - 2012
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/418
AB  - A sensitive real-time PCR technique was used to examine the distribution of Toxoplasma gondii in the blood and tissues of mice during acute and chronic infection. Groups of Swiss Albino mice, inoculated i.p. with 10(2) or 10(6) tachyzoites of the RH strain as a typical type-1 strain, or fed 10 cysts of the Me49 strain as a typical type-2 strain, were killed at different time points post-infection (p.i.), and blood and organs including the lungs, brain and liver were harvested for DNA extraction. Toxoplasma DNA was quantified by a real-time PCR targeted at the 529 bp gene fragment, with a detection limit of a single parasite per g/ml of tissue. The results showed a strain- and dose-dependent spread of Toxoplasma. In infection with type-1 parasites, in case of a high infective dose, Toxoplasma DNA was detected within 24 h p.i. in all analyzed tissues including the brain. Conversely, in case of a low infective dose, parasitaemia was undetectable early p.i., at a time when Toxoplasma DNA was detected in the tissues, but reached very high levels as infection progressed. With both infective doses, pre-death parasite burdens were higher in the blood than in the tissues, whereas the same loads in the lungs suggest that reaching these Toxoplasma burdens may be critical for survival. In infection with Me49 parasites, steady high parasite burdens were noted up to the end of the experiment at d42 only in the brain, parasitaemia was low but detectable throughout, and Toxoplasma DNA was completely cleared only from the liver. These data are important to better understand the pathogenesis of toxoplasmosis, and also as baseline data for the experimental evaluation of novel chemotherapeutics.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Experimental Parasitology
T1  - Kinetics of parasite burdens in blood and tissues during murine toxoplasmosis
EP  - 376
IS  - 3
SP  - 372
VL  - 131
DO  - 10.1016/j.exppara.2012.05.006
UR  - conv_2780
ER  - 
@article{
author = "Đurković-Đaković, Olgica and Đokić, Vitomir and Vujanić, Marija and Živković, Tijana and Bobić, Branko and Nikolić, Aleksandra and Slavić, Ksenija and Klun, Ivana and Ivović, Vladimir",
year = "2012",
abstract = "A sensitive real-time PCR technique was used to examine the distribution of Toxoplasma gondii in the blood and tissues of mice during acute and chronic infection. Groups of Swiss Albino mice, inoculated i.p. with 10(2) or 10(6) tachyzoites of the RH strain as a typical type-1 strain, or fed 10 cysts of the Me49 strain as a typical type-2 strain, were killed at different time points post-infection (p.i.), and blood and organs including the lungs, brain and liver were harvested for DNA extraction. Toxoplasma DNA was quantified by a real-time PCR targeted at the 529 bp gene fragment, with a detection limit of a single parasite per g/ml of tissue. The results showed a strain- and dose-dependent spread of Toxoplasma. In infection with type-1 parasites, in case of a high infective dose, Toxoplasma DNA was detected within 24 h p.i. in all analyzed tissues including the brain. Conversely, in case of a low infective dose, parasitaemia was undetectable early p.i., at a time when Toxoplasma DNA was detected in the tissues, but reached very high levels as infection progressed. With both infective doses, pre-death parasite burdens were higher in the blood than in the tissues, whereas the same loads in the lungs suggest that reaching these Toxoplasma burdens may be critical for survival. In infection with Me49 parasites, steady high parasite burdens were noted up to the end of the experiment at d42 only in the brain, parasitaemia was low but detectable throughout, and Toxoplasma DNA was completely cleared only from the liver. These data are important to better understand the pathogenesis of toxoplasmosis, and also as baseline data for the experimental evaluation of novel chemotherapeutics.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Experimental Parasitology",
title = "Kinetics of parasite burdens in blood and tissues during murine toxoplasmosis",
pages = "376-372",
number = "3",
volume = "131",
doi = "10.1016/j.exppara.2012.05.006",
url = "conv_2780"
}
Đurković-Đaković, O., Đokić, V., Vujanić, M., Živković, T., Bobić, B., Nikolić, A., Slavić, K., Klun, I.,& Ivović, V.. (2012). Kinetics of parasite burdens in blood and tissues during murine toxoplasmosis. in Experimental Parasitology
Academic Press Inc Elsevier Science, San Diego., 131(3), 372-376.
https://doi.org/10.1016/j.exppara.2012.05.006
conv_2780
Đurković-Đaković O, Đokić V, Vujanić M, Živković T, Bobić B, Nikolić A, Slavić K, Klun I, Ivović V. Kinetics of parasite burdens in blood and tissues during murine toxoplasmosis. in Experimental Parasitology. 2012;131(3):372-376.
doi:10.1016/j.exppara.2012.05.006
conv_2780 .
Đurković-Đaković, Olgica, Đokić, Vitomir, Vujanić, Marija, Živković, Tijana, Bobić, Branko, Nikolić, Aleksandra, Slavić, Ksenija, Klun, Ivana, Ivović, Vladimir, "Kinetics of parasite burdens in blood and tissues during murine toxoplasmosis" in Experimental Parasitology, 131, no. 3 (2012):372-376,
https://doi.org/10.1016/j.exppara.2012.05.006 .,
conv_2780 .

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