In order to evaluate the prevalence rate of Babesia spp. in ticks collected from vegetation at seven localities in northern Serbia, tick samples were subjected to molecular analysis. A total of 132 unfed adult ticks of five different species (Dermacentor marginatus, Dermacentor reticulatus, Ixodes ricinus, Haemaphysalis concinna and Haemaphysalis punctata), were examined by PCR for the presence of Babesia spp. Out of the analyzed ticks, 10.61% (14/132) were positive for babesial DNA. The presence of babesiae was found at the localities Pančevački Rit, Titov Gaj, Makiš, PKB and Kljajićevo. Prevalence in D. reticulatus ticks was 21.57% (11/51) and in H. concinna ticks, 8.57% (3/35). Sequencing and phylogenetic analysis showed a clustering of the obtained sequences with those of B. canis from the GenBank database. These results add to the knowledge of the distribution of babesial pathogens and their vectors in Serbia.
Keywords:
ticks / PCR / Babesia spp. / Serbia
Source:
Archives of Biological Sciences, 2012, 64, 4, 1591-1598
TY - JOUR
AU - Mihaljica, Darko
AU - Radulović, Željko
AU - Tomanović, Snežana
AU - Ćakić, Sanja
AU - Penezić, Aleksandra
AU - Milutinović, Marija
PY - 2012
UR - http://rimi.imi.bg.ac.rs/handle/123456789/407
AB - In order to evaluate the prevalence rate of Babesia spp. in ticks collected from vegetation at seven localities in northern Serbia, tick samples were subjected to molecular analysis. A total of 132 unfed adult ticks of five different species (Dermacentor marginatus, Dermacentor reticulatus, Ixodes ricinus, Haemaphysalis concinna and Haemaphysalis punctata), were examined by PCR for the presence of Babesia spp. Out of the analyzed ticks, 10.61% (14/132) were positive for babesial DNA. The presence of babesiae was found at the localities Pančevački Rit, Titov Gaj, Makiš, PKB and Kljajićevo. Prevalence in D. reticulatus ticks was 21.57% (11/51) and in H. concinna ticks, 8.57% (3/35). Sequencing and phylogenetic analysis showed a clustering of the obtained sequences with those of B. canis from the GenBank database. These results add to the knowledge of the distribution of babesial pathogens and their vectors in Serbia.
PB - Srpsko biološko društvo, Beograd, i dr.
T2 - Archives of Biological Sciences
T1 - Molecular detection of Babesia spp. in ticks in northern Serbia
EP - 1598
IS - 4
SP - 1591
VL - 64
DO - 10.2298/ABS1204591M
UR - conv_2873
ER -
@article{
author = "Mihaljica, Darko and Radulović, Željko and Tomanović, Snežana and Ćakić, Sanja and Penezić, Aleksandra and Milutinović, Marija",
year = "2012",
abstract = "In order to evaluate the prevalence rate of Babesia spp. in ticks collected from vegetation at seven localities in northern Serbia, tick samples were subjected to molecular analysis. A total of 132 unfed adult ticks of five different species (Dermacentor marginatus, Dermacentor reticulatus, Ixodes ricinus, Haemaphysalis concinna and Haemaphysalis punctata), were examined by PCR for the presence of Babesia spp. Out of the analyzed ticks, 10.61% (14/132) were positive for babesial DNA. The presence of babesiae was found at the localities Pančevački Rit, Titov Gaj, Makiš, PKB and Kljajićevo. Prevalence in D. reticulatus ticks was 21.57% (11/51) and in H. concinna ticks, 8.57% (3/35). Sequencing and phylogenetic analysis showed a clustering of the obtained sequences with those of B. canis from the GenBank database. These results add to the knowledge of the distribution of babesial pathogens and their vectors in Serbia.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Molecular detection of Babesia spp. in ticks in northern Serbia",
pages = "1598-1591",
number = "4",
volume = "64",
doi = "10.2298/ABS1204591M",
url = "conv_2873"
}
Mihaljica, D., Radulović, Ž., Tomanović, S., Ćakić, S., Penezić, A.,& Milutinović, M.. (2012). Molecular detection of Babesia spp. in ticks in northern Serbia. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 64(4), 1591-1598.
https://doi.org/10.2298/ABS1204591M
conv_2873
Mihaljica D, Radulović Ž, Tomanović S, Ćakić S, Penezić A, Milutinović M. Molecular detection of Babesia spp. in ticks in northern Serbia. in Archives of Biological Sciences. 2012;64(4):1591-1598.
doi:10.2298/ABS1204591M
conv_2873 .
Mihaljica, Darko, Radulović, Željko, Tomanović, Snežana, Ćakić, Sanja, Penezić, Aleksandra, Milutinović, Marija, "Molecular detection of Babesia spp. in ticks in northern Serbia" in Archives of Biological Sciences, 64, no. 4 (2012):1591-1598,
https://doi.org/10.2298/ABS1204591M .,
conv_2873 .
