Приказ основних података о документу

dc.creatorPetrović, Snježana
dc.creatorMilošević, Maja
dc.creatorDrakulić, Dunja
dc.creatorGrković, Ivana
dc.creatorStanojlovio, M.
dc.creatorMitrović, N.
dc.creatorHorvat, Anica
dc.date.accessioned2021-04-20T12:28:12Z
dc.date.available2021-04-20T12:28:12Z
dc.date.issued2012
dc.identifier.issn0306-4522
dc.identifier.urihttp://rimi.imi.bg.ac.rs/handle/123456789/400
dc.description.abstractThe aim of this study was to examine the rapid non-genomic effect of 17 beta-estradiol (E2) on Ca2+ transport in mitochondria isolated from the nerve terminals (synaptosomes) of caudate nuclei (NC) and brain stems (BS) of ovariectomised female rats. In physiological conditions no effect of E2 on Ca2+ influx into synaptosomal mitochondria through ruthenium red (RR)-sensitive uniporter was observed. However, in the presence of uncoupling agent carbonyl cyanide4-(trifluoromethoxy)phenylhydrazone (FCCP) (1 mu mol/l), pre-treatment with 0.5 nmol/l E2 protected mitochondrial membrane potential and consequently increased Ca2+ influx (2.3-fold in NC and 3.1-fold in BS). At the same time, 0.5 nmol/l E2 by increasing the affinity of mitochondrial Na+/Ca2+ exchanger for Na+ inhibited mitochondrial Ca2+ efflux in NC and BS by about 40%. Also, the specific binding of physiological E2 concentrations (0.1-10 nmol/l) to isolated synaptosomal mitochondria was detected. Using membrane impermeable E2 bound to bovine serum albumin and selective inhibitor of mitochondrial Na+/Ca2+ exchanger, we obtained that E2's action on mitochondrial Ca2+ efflux at least partially is due to the direct effects on the mitochondrial membrane and/or Na+/Ca2+ exchanger located in inner mitochondrial membrane. Our results implicate E2 as a modulator of Ca2+ concentration in mitochondrial matrix, and ultimately in the cytosol. Given the vital role of Ca2+ in regulation of total nerve cells activity, especially energy metabolism, neurotransmission and directing the cells toward survival or cell death, the effects on mitochondrial Ca2+ transport could be one of the important modes of E2 neuromodulatory action independent of the genome.en
dc.publisherPergamon-Elsevier Science Ltd, Oxford
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173044/RS//
dc.rightsrestrictedAccess
dc.sourceNeuroscience
dc.subjectsynaptosomal mitochondriaen
dc.subjectCa2+ transporten
dc.subjectestradiolen
dc.subjectrat brainen
dc.subjectcaudate nucleusen
dc.subjectbrain stemen
dc.title17 beta-estradiol modulates mitochondrial ca(2+) flux in rat caudate nucleus and brain stemen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage40
dc.citation.other220: 32-40
dc.citation.rankM22
dc.citation.spage32
dc.citation.volume220
dc.identifier.doi10.1016/j.neuroscience.2012.06.040
dc.identifier.pmid22735576
dc.identifier.scopus2-s2.0-84864780476
dc.identifier.wos000307802100005
dc.type.versionpublishedVersion


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Приказ основних података о документу