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Evidence that calf bronchopneumonia may be accompanied by increased sialylation of circulating immune complexes' IgG

Authorized Users Only
2012
Authors
Fratrić, Natalija
Gvozdić, Dragan
Vuković, Dejan
Savić, Olivera
Kovačić, Marijana
Ilić, Vesna
Article (Published version)
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Abstract
Immune complexes (IC) could have an important role in the pathogenesis of pre-ruminant calves' bronchopneumonia. IC are potent activators of complement and neutrophils and they might be responsible for immune protection, as well as for pulmonary damage. Immunoglobulin G (IgG), as constituents of IC, initiates the effector phase of immune response through binding of Fc gamma and complement receptors. The oligosaccharide moieties expressed on IgG can modulate their antigen affinity and effector function. Structural characteristics of IgG molecules from IC in the pre-ruminant calves have not been studied in detail. The aim of our study was to determine if the glycosylation profile of IgG from circulating IC (CIC) in calves with bronchopneumonia differed from those of healthy control calves. A total number of 13 Holstein-Friesian calves, at the age of three months were included in the study. All calves were clinically examined by a veterinarian. Calves were classified by signs of respirato...ry disease in two groups: healthy (n = 6) and diseased (n = 7) calves. The CIC from calves' sera were isolated by the polyethylene glycol precipitation (PEG) method. IgG molecules were isolated from PEG precipitates by Protein G affinity method. The level of expression and localization N-acetylglucosamine, galactose, sialic acid, and fucose within the isolated IgG was determined by lectin blot assay. Calves with bronchopneumonia had a statistically significantly increased level of CIC. IgG molecule:, were isolated from CIC of both healthy and diseased calves. Several other proteins in complex with IgG were detected in both groups of animals. The isolated IgG heavy chains of healthy calves expressed N-acetylglucosamine, galactose, sialic acid, and fucose. The light chains of IgG expressed N-acetylglucosamine, sialic acid, and fucose whereas galactose was not detected in healthy calves. In diseased animals, galactose was detected on light chains, and both heavy and light IgG chains were more sialylated. Proteins in complex with IgG were also lectin reactive, and their glycosylation in diseased animals was different compared to healthy controls. Increased sialylation is a characteristic of anti-inflammatory IgG. The increased sialylation of IgG from CIC in bronchopneumonia might be an attempt of immune system of calves to protect lung tissues against damages provoked by activated cells and secreted pro-inflammatory cytokines. At the same time, increased IgG sialylation could explain the inability of calves' immune system to initiate the process of antigen elimination by activation of Fc gamma receptors.

Keywords:
Calves / Bronchopneumonia / Immune complexes / IgG / Oligosaccharide / Sialylation
Source:
Veterinary Immunology & Immunopathology, 2012, 150, 3-4, 161-168
Publisher:
  • Elsevier, Amsterdam
Funding / projects:
  • Molecular genetic and ecophysiological researches on the protection of autochthonous animal genetic resources, sustaining domestic animals’ welfare, health and reproduction, and safe food production (RS-46002)
  • Regenerative and modulatory potential of adult stem cells (RS-175062)
  • Biotechnology in the regulation of productive and reproductive status and health in dairy cows (RS-31050)

DOI: 10.1016/j.vetimm.2012.09.009

ISSN: 0165-2427

PubMed: 23068275

WoS: 000312052500003

Scopus: 2-s2.0-84868451890
[ Google Scholar ]
6
6
URI
http://rimi.imi.bg.ac.rs/handle/123456789/399
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
Institut za medicinska istraživanja
TY  - JOUR
AU  - Fratrić, Natalija
AU  - Gvozdić, Dragan
AU  - Vuković, Dejan
AU  - Savić, Olivera
AU  - Kovačić, Marijana
AU  - Ilić, Vesna
PY  - 2012
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/399
AB  - Immune complexes (IC) could have an important role in the pathogenesis of pre-ruminant calves' bronchopneumonia. IC are potent activators of complement and neutrophils and they might be responsible for immune protection, as well as for pulmonary damage. Immunoglobulin G (IgG), as constituents of IC, initiates the effector phase of immune response through binding of Fc gamma and complement receptors. The oligosaccharide moieties expressed on IgG can modulate their antigen affinity and effector function. Structural characteristics of IgG molecules from IC in the pre-ruminant calves have not been studied in detail. The aim of our study was to determine if the glycosylation profile of IgG from circulating IC (CIC) in calves with bronchopneumonia differed from those of healthy control calves. A total number of 13 Holstein-Friesian calves, at the age of three months were included in the study. All calves were clinically examined by a veterinarian. Calves were classified by signs of respiratory disease in two groups: healthy (n = 6) and diseased (n = 7) calves. The CIC from calves' sera were isolated by the polyethylene glycol precipitation (PEG) method. IgG molecules were isolated from PEG precipitates by Protein G affinity method. The level of expression and localization N-acetylglucosamine, galactose, sialic acid, and fucose within the isolated IgG was determined by lectin blot assay. Calves with bronchopneumonia had a statistically significantly increased level of CIC. IgG molecule:, were isolated from CIC of both healthy and diseased calves. Several other proteins in complex with IgG were detected in both groups of animals. The isolated IgG heavy chains of healthy calves expressed N-acetylglucosamine, galactose, sialic acid, and fucose. The light chains of IgG expressed N-acetylglucosamine, sialic acid, and fucose whereas galactose was not detected in healthy calves. In diseased animals, galactose was detected on light chains, and both heavy and light IgG chains were more sialylated. Proteins in complex with IgG were also lectin reactive, and their glycosylation in diseased animals was different compared to healthy controls. Increased sialylation is a characteristic of anti-inflammatory IgG. The increased sialylation of IgG from CIC in bronchopneumonia might be an attempt of immune system of calves to protect lung tissues against damages provoked by activated cells and secreted pro-inflammatory cytokines. At the same time, increased IgG sialylation could explain the inability of calves' immune system to initiate the process of antigen elimination by activation of Fc gamma receptors.
PB  - Elsevier, Amsterdam
T2  - Veterinary Immunology & Immunopathology
T1  - Evidence that calf bronchopneumonia may be accompanied by increased sialylation of circulating immune complexes' IgG
EP  - 168
IS  - 3-4
SP  - 161
VL  - 150
DO  - 10.1016/j.vetimm.2012.09.009
UR  - conv_2876
ER  - 
@article{
author = "Fratrić, Natalija and Gvozdić, Dragan and Vuković, Dejan and Savić, Olivera and Kovačić, Marijana and Ilić, Vesna",
year = "2012",
abstract = "Immune complexes (IC) could have an important role in the pathogenesis of pre-ruminant calves' bronchopneumonia. IC are potent activators of complement and neutrophils and they might be responsible for immune protection, as well as for pulmonary damage. Immunoglobulin G (IgG), as constituents of IC, initiates the effector phase of immune response through binding of Fc gamma and complement receptors. The oligosaccharide moieties expressed on IgG can modulate their antigen affinity and effector function. Structural characteristics of IgG molecules from IC in the pre-ruminant calves have not been studied in detail. The aim of our study was to determine if the glycosylation profile of IgG from circulating IC (CIC) in calves with bronchopneumonia differed from those of healthy control calves. A total number of 13 Holstein-Friesian calves, at the age of three months were included in the study. All calves were clinically examined by a veterinarian. Calves were classified by signs of respiratory disease in two groups: healthy (n = 6) and diseased (n = 7) calves. The CIC from calves' sera were isolated by the polyethylene glycol precipitation (PEG) method. IgG molecules were isolated from PEG precipitates by Protein G affinity method. The level of expression and localization N-acetylglucosamine, galactose, sialic acid, and fucose within the isolated IgG was determined by lectin blot assay. Calves with bronchopneumonia had a statistically significantly increased level of CIC. IgG molecule:, were isolated from CIC of both healthy and diseased calves. Several other proteins in complex with IgG were detected in both groups of animals. The isolated IgG heavy chains of healthy calves expressed N-acetylglucosamine, galactose, sialic acid, and fucose. The light chains of IgG expressed N-acetylglucosamine, sialic acid, and fucose whereas galactose was not detected in healthy calves. In diseased animals, galactose was detected on light chains, and both heavy and light IgG chains were more sialylated. Proteins in complex with IgG were also lectin reactive, and their glycosylation in diseased animals was different compared to healthy controls. Increased sialylation is a characteristic of anti-inflammatory IgG. The increased sialylation of IgG from CIC in bronchopneumonia might be an attempt of immune system of calves to protect lung tissues against damages provoked by activated cells and secreted pro-inflammatory cytokines. At the same time, increased IgG sialylation could explain the inability of calves' immune system to initiate the process of antigen elimination by activation of Fc gamma receptors.",
publisher = "Elsevier, Amsterdam",
journal = "Veterinary Immunology & Immunopathology",
title = "Evidence that calf bronchopneumonia may be accompanied by increased sialylation of circulating immune complexes' IgG",
pages = "168-161",
number = "3-4",
volume = "150",
doi = "10.1016/j.vetimm.2012.09.009",
url = "conv_2876"
}
Fratrić, N., Gvozdić, D., Vuković, D., Savić, O., Kovačić, M.,& Ilić, V.. (2012). Evidence that calf bronchopneumonia may be accompanied by increased sialylation of circulating immune complexes' IgG. in Veterinary Immunology & Immunopathology
Elsevier, Amsterdam., 150(3-4), 161-168.
https://doi.org/10.1016/j.vetimm.2012.09.009
conv_2876
Fratrić N, Gvozdić D, Vuković D, Savić O, Kovačić M, Ilić V. Evidence that calf bronchopneumonia may be accompanied by increased sialylation of circulating immune complexes' IgG. in Veterinary Immunology & Immunopathology. 2012;150(3-4):161-168.
doi:10.1016/j.vetimm.2012.09.009
conv_2876 .
Fratrić, Natalija, Gvozdić, Dragan, Vuković, Dejan, Savić, Olivera, Kovačić, Marijana, Ilić, Vesna, "Evidence that calf bronchopneumonia may be accompanied by increased sialylation of circulating immune complexes' IgG" in Veterinary Immunology & Immunopathology, 150, no. 3-4 (2012):161-168,
https://doi.org/10.1016/j.vetimm.2012.09.009 .,
conv_2876 .

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