Ethanol and nitric oxide modulate expression of glucocorticoid receptor in the rat adrenal cortex
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2012
Authors
Đikić, DragoslavaBudeč, Mirela

Vranješ-Đurić, Sanja

Todorović, Vera
Drndarević, Neda
Vignjević, Sanja

Mitrović, Olivera

Article (Published version)

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Background: This study was performed to investigate expression and distribution of glucocorticoid receptor (GR) in the rat adrenal cortex, acute effect of ethanol on its expression and possible role of endogenous nitric oxide (NO) in this phenomenon. Methods: Adult female Wistar rats showing diestrus day 1 were treated with: a) ethanol (2 or 4 g/kg body weight (b.w.), ip), b) N-omega-nitro-L-arginine methyl ester (L-NAME), well-known competitive inhibitor of all isoforms of NO synthase (NOS), (30 mg/kg b.w., sc) followed by ethanol (4 g/kg, ip) 3 h later and c) L-NAME (30 mg/kg b.w., sc) followed by saline (ip) 3 h later. Untreated rats were used as controls. Adrenocortical expression of GR was estimated by immunohistochemistry. Results: Strong nuclear GR staining was observed throughout the cortex of control rats. Acute ethanol treatment significantly decreased the expression of GR in the zona fasciculata and zona reticularis. Blockade of NO formation had no influence on this effect o...f ethanol, whereas L-NAME itself induced significant decline in GR immunoreactivity. Conclusions: Obtained findings are the first to demonstrate localization and distribution of the GR throughout the rat adrenal cortex and to suggest that ethanol as well as endogenous NO may modulate adrenocortical expression of this steroid receptor.
Keywords:
ethanol / L-NAME / glucocorticoid receptor / adrenal cortexSource:
Pharmacological Reports, 2012, 64, 4, 896-901Publisher:
- Polish Acad Sciences Inst Pharmacology, Krakow
Funding / projects:
DOI: 10.1016/S1734-1140(12)70884-8
ISSN: 1734-1140
PubMed: 23087141
WoS: 000310420500012
Scopus: 2-s2.0-84867678044
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Institut za medicinska istraživanjaTY - JOUR AU - Đikić, Dragoslava AU - Budeč, Mirela AU - Vranješ-Đurić, Sanja AU - Todorović, Vera AU - Drndarević, Neda AU - Vignjević, Sanja AU - Mitrović, Olivera PY - 2012 UR - http://rimi.imi.bg.ac.rs/handle/123456789/393 AB - Background: This study was performed to investigate expression and distribution of glucocorticoid receptor (GR) in the rat adrenal cortex, acute effect of ethanol on its expression and possible role of endogenous nitric oxide (NO) in this phenomenon. Methods: Adult female Wistar rats showing diestrus day 1 were treated with: a) ethanol (2 or 4 g/kg body weight (b.w.), ip), b) N-omega-nitro-L-arginine methyl ester (L-NAME), well-known competitive inhibitor of all isoforms of NO synthase (NOS), (30 mg/kg b.w., sc) followed by ethanol (4 g/kg, ip) 3 h later and c) L-NAME (30 mg/kg b.w., sc) followed by saline (ip) 3 h later. Untreated rats were used as controls. Adrenocortical expression of GR was estimated by immunohistochemistry. Results: Strong nuclear GR staining was observed throughout the cortex of control rats. Acute ethanol treatment significantly decreased the expression of GR in the zona fasciculata and zona reticularis. Blockade of NO formation had no influence on this effect of ethanol, whereas L-NAME itself induced significant decline in GR immunoreactivity. Conclusions: Obtained findings are the first to demonstrate localization and distribution of the GR throughout the rat adrenal cortex and to suggest that ethanol as well as endogenous NO may modulate adrenocortical expression of this steroid receptor. PB - Polish Acad Sciences Inst Pharmacology, Krakow T2 - Pharmacological Reports T1 - Ethanol and nitric oxide modulate expression of glucocorticoid receptor in the rat adrenal cortex EP - 901 IS - 4 SP - 896 VL - 64 DO - 10.1016/S1734-1140(12)70884-8 ER -
@article{ author = "Đikić, Dragoslava and Budeč, Mirela and Vranješ-Đurić, Sanja and Todorović, Vera and Drndarević, Neda and Vignjević, Sanja and Mitrović, Olivera", year = "2012", abstract = "Background: This study was performed to investigate expression and distribution of glucocorticoid receptor (GR) in the rat adrenal cortex, acute effect of ethanol on its expression and possible role of endogenous nitric oxide (NO) in this phenomenon. Methods: Adult female Wistar rats showing diestrus day 1 were treated with: a) ethanol (2 or 4 g/kg body weight (b.w.), ip), b) N-omega-nitro-L-arginine methyl ester (L-NAME), well-known competitive inhibitor of all isoforms of NO synthase (NOS), (30 mg/kg b.w., sc) followed by ethanol (4 g/kg, ip) 3 h later and c) L-NAME (30 mg/kg b.w., sc) followed by saline (ip) 3 h later. Untreated rats were used as controls. Adrenocortical expression of GR was estimated by immunohistochemistry. Results: Strong nuclear GR staining was observed throughout the cortex of control rats. Acute ethanol treatment significantly decreased the expression of GR in the zona fasciculata and zona reticularis. Blockade of NO formation had no influence on this effect of ethanol, whereas L-NAME itself induced significant decline in GR immunoreactivity. Conclusions: Obtained findings are the first to demonstrate localization and distribution of the GR throughout the rat adrenal cortex and to suggest that ethanol as well as endogenous NO may modulate adrenocortical expression of this steroid receptor.", publisher = "Polish Acad Sciences Inst Pharmacology, Krakow", journal = "Pharmacological Reports", title = "Ethanol and nitric oxide modulate expression of glucocorticoid receptor in the rat adrenal cortex", pages = "901-896", number = "4", volume = "64", doi = "10.1016/S1734-1140(12)70884-8" }
Đikić, D., Budeč, M., Vranješ-Đurić, S., Todorović, V., Drndarević, N., Vignjević, S.,& Mitrović, O.. (2012). Ethanol and nitric oxide modulate expression of glucocorticoid receptor in the rat adrenal cortex. in Pharmacological Reports Polish Acad Sciences Inst Pharmacology, Krakow., 64(4), 896-901. https://doi.org/10.1016/S1734-1140(12)70884-8
Đikić D, Budeč M, Vranješ-Đurić S, Todorović V, Drndarević N, Vignjević S, Mitrović O. Ethanol and nitric oxide modulate expression of glucocorticoid receptor in the rat adrenal cortex. in Pharmacological Reports. 2012;64(4):896-901. doi:10.1016/S1734-1140(12)70884-8 .
Đikić, Dragoslava, Budeč, Mirela, Vranješ-Đurić, Sanja, Todorović, Vera, Drndarević, Neda, Vignjević, Sanja, Mitrović, Olivera, "Ethanol and nitric oxide modulate expression of glucocorticoid receptor in the rat adrenal cortex" in Pharmacological Reports, 64, no. 4 (2012):896-901, https://doi.org/10.1016/S1734-1140(12)70884-8 . .