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Ethanol and nitric oxide modulate expression of glucocorticoid receptor in the rat adrenal cortex

Authorized Users Only
2012
Authors
Đikić, Dragoslava
Budeč, Mirela
Vranješ-Đurić, Sanja
Todorović, Vera
Drndarević, Neda
Vignjević, Sanja
Mitrović, Olivera
Article (Published version)
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Abstract
Background: This study was performed to investigate expression and distribution of glucocorticoid receptor (GR) in the rat adrenal cortex, acute effect of ethanol on its expression and possible role of endogenous nitric oxide (NO) in this phenomenon. Methods: Adult female Wistar rats showing diestrus day 1 were treated with: a) ethanol (2 or 4 g/kg body weight (b.w.), ip), b) N-omega-nitro-L-arginine methyl ester (L-NAME), well-known competitive inhibitor of all isoforms of NO synthase (NOS), (30 mg/kg b.w., sc) followed by ethanol (4 g/kg, ip) 3 h later and c) L-NAME (30 mg/kg b.w., sc) followed by saline (ip) 3 h later. Untreated rats were used as controls. Adrenocortical expression of GR was estimated by immunohistochemistry. Results: Strong nuclear GR staining was observed throughout the cortex of control rats. Acute ethanol treatment significantly decreased the expression of GR in the zona fasciculata and zona reticularis. Blockade of NO formation had no influence on this effect o...f ethanol, whereas L-NAME itself induced significant decline in GR immunoreactivity. Conclusions: Obtained findings are the first to demonstrate localization and distribution of the GR throughout the rat adrenal cortex and to suggest that ethanol as well as endogenous NO may modulate adrenocortical expression of this steroid receptor.

Keywords:
ethanol / L-NAME / glucocorticoid receptor / adrenal cortex
Source:
Pharmacological Reports, 2012, 64, 4, 896-901
Publisher:
  • Polish Acad Sciences Inst Pharmacology, Krakow
Funding / projects:
  • The pathogenetic mechanism in hematological malignancies (RS-175053)

DOI: 10.1016/S1734-1140(12)70884-8

ISSN: 1734-1140

PubMed: 23087141

WoS: 000310420500012

Scopus: 2-s2.0-84867678044
[ Google Scholar ]
5
5
URI
http://rimi.imi.bg.ac.rs/handle/123456789/393
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
Institut za medicinska istraživanja
TY  - JOUR
AU  - Đikić, Dragoslava
AU  - Budeč, Mirela
AU  - Vranješ-Đurić, Sanja
AU  - Todorović, Vera
AU  - Drndarević, Neda
AU  - Vignjević, Sanja
AU  - Mitrović, Olivera
PY  - 2012
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/393
AB  - Background: This study was performed to investigate expression and distribution of glucocorticoid receptor (GR) in the rat adrenal cortex, acute effect of ethanol on its expression and possible role of endogenous nitric oxide (NO) in this phenomenon. Methods: Adult female Wistar rats showing diestrus day 1 were treated with: a) ethanol (2 or 4 g/kg body weight (b.w.), ip), b) N-omega-nitro-L-arginine methyl ester (L-NAME), well-known competitive inhibitor of all isoforms of NO synthase (NOS), (30 mg/kg b.w., sc) followed by ethanol (4 g/kg, ip) 3 h later and c) L-NAME (30 mg/kg b.w., sc) followed by saline (ip) 3 h later. Untreated rats were used as controls. Adrenocortical expression of GR was estimated by immunohistochemistry. Results: Strong nuclear GR staining was observed throughout the cortex of control rats. Acute ethanol treatment significantly decreased the expression of GR in the zona fasciculata and zona reticularis. Blockade of NO formation had no influence on this effect of ethanol, whereas L-NAME itself induced significant decline in GR immunoreactivity. Conclusions: Obtained findings are the first to demonstrate localization and distribution of the GR throughout the rat adrenal cortex and to suggest that ethanol as well as endogenous NO may modulate adrenocortical expression of this steroid receptor.
PB  - Polish Acad Sciences Inst Pharmacology, Krakow
T2  - Pharmacological Reports
T1  - Ethanol and nitric oxide modulate expression of glucocorticoid receptor in the rat adrenal cortex
EP  - 901
IS  - 4
SP  - 896
VL  - 64
DO  - 10.1016/S1734-1140(12)70884-8
UR  - conv_2851
ER  - 
@article{
author = "Đikić, Dragoslava and Budeč, Mirela and Vranješ-Đurić, Sanja and Todorović, Vera and Drndarević, Neda and Vignjević, Sanja and Mitrović, Olivera",
year = "2012",
abstract = "Background: This study was performed to investigate expression and distribution of glucocorticoid receptor (GR) in the rat adrenal cortex, acute effect of ethanol on its expression and possible role of endogenous nitric oxide (NO) in this phenomenon. Methods: Adult female Wistar rats showing diestrus day 1 were treated with: a) ethanol (2 or 4 g/kg body weight (b.w.), ip), b) N-omega-nitro-L-arginine methyl ester (L-NAME), well-known competitive inhibitor of all isoforms of NO synthase (NOS), (30 mg/kg b.w., sc) followed by ethanol (4 g/kg, ip) 3 h later and c) L-NAME (30 mg/kg b.w., sc) followed by saline (ip) 3 h later. Untreated rats were used as controls. Adrenocortical expression of GR was estimated by immunohistochemistry. Results: Strong nuclear GR staining was observed throughout the cortex of control rats. Acute ethanol treatment significantly decreased the expression of GR in the zona fasciculata and zona reticularis. Blockade of NO formation had no influence on this effect of ethanol, whereas L-NAME itself induced significant decline in GR immunoreactivity. Conclusions: Obtained findings are the first to demonstrate localization and distribution of the GR throughout the rat adrenal cortex and to suggest that ethanol as well as endogenous NO may modulate adrenocortical expression of this steroid receptor.",
publisher = "Polish Acad Sciences Inst Pharmacology, Krakow",
journal = "Pharmacological Reports",
title = "Ethanol and nitric oxide modulate expression of glucocorticoid receptor in the rat adrenal cortex",
pages = "901-896",
number = "4",
volume = "64",
doi = "10.1016/S1734-1140(12)70884-8",
url = "conv_2851"
}
Đikić, D., Budeč, M., Vranješ-Đurić, S., Todorović, V., Drndarević, N., Vignjević, S.,& Mitrović, O.. (2012). Ethanol and nitric oxide modulate expression of glucocorticoid receptor in the rat adrenal cortex. in Pharmacological Reports
Polish Acad Sciences Inst Pharmacology, Krakow., 64(4), 896-901.
https://doi.org/10.1016/S1734-1140(12)70884-8
conv_2851
Đikić D, Budeč M, Vranješ-Đurić S, Todorović V, Drndarević N, Vignjević S, Mitrović O. Ethanol and nitric oxide modulate expression of glucocorticoid receptor in the rat adrenal cortex. in Pharmacological Reports. 2012;64(4):896-901.
doi:10.1016/S1734-1140(12)70884-8
conv_2851 .
Đikić, Dragoslava, Budeč, Mirela, Vranješ-Đurić, Sanja, Todorović, Vera, Drndarević, Neda, Vignjević, Sanja, Mitrović, Olivera, "Ethanol and nitric oxide modulate expression of glucocorticoid receptor in the rat adrenal cortex" in Pharmacological Reports, 64, no. 4 (2012):896-901,
https://doi.org/10.1016/S1734-1140(12)70884-8 .,
conv_2851 .

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