The present study evaluated the role of interleukin (IL) 17 in multilineage commitment of C2C12 myoblastic cells and investigated associated signaling pathways. The results concerning the effects on cell function showed that IL-17 inhibits the migration of C2C12 cells, while not affecting their proliferation. The data regarding the influence on differentiation demonstrated that IL-17 inhibits myogenic differentiation of C2C12 cells by down-regulating the myogenin mRNA level, myosin heavy chain expression and myotube formation, but promotes their osteogenic differentiation by up-regulating the Runt-related transcription factor 2 mRNA level, cyclooxygenase-2 expression and alkaline phosphatase activity. IL-17 exerted these effects by activating ERK1,2 mitogen activated protein kinase signaling pathway, which in turn regulated the expression of relevant genes and proteins to inhibit myogenic differentiation and induce osteogenic differentiation. Additional analysis showed that the inducti...on of osteogenic differentiation by IL-17 is independent of BMP signaling. The results obtained demonstrate the potential of IL-17 not only to inhibit the myogenic differentiation of C2C12 myoblasts but also to convert their differentiation pathway into that of osteoblast lineage providing new insight into the capacities of IL-17 to modulate the differentiation commitment.
TY - JOUR
AU - Krstić, Jelena
AU - Santibanez, Juan
AU - Krstić, Aleksandra
AU - Mojsilović, Slavko
AU - Okić-Đorđević, Ivana
AU - Trivanović, Drenka
AU - Ilić, Vesna
AU - Bugarski, Diana
PY - 2012
UR - http://rimi.imi.bg.ac.rs/handle/123456789/389
AB - The present study evaluated the role of interleukin (IL) 17 in multilineage commitment of C2C12 myoblastic cells and investigated associated signaling pathways. The results concerning the effects on cell function showed that IL-17 inhibits the migration of C2C12 cells, while not affecting their proliferation. The data regarding the influence on differentiation demonstrated that IL-17 inhibits myogenic differentiation of C2C12 cells by down-regulating the myogenin mRNA level, myosin heavy chain expression and myotube formation, but promotes their osteogenic differentiation by up-regulating the Runt-related transcription factor 2 mRNA level, cyclooxygenase-2 expression and alkaline phosphatase activity. IL-17 exerted these effects by activating ERK1,2 mitogen activated protein kinase signaling pathway, which in turn regulated the expression of relevant genes and proteins to inhibit myogenic differentiation and induce osteogenic differentiation. Additional analysis showed that the induction of osteogenic differentiation by IL-17 is independent of BMP signaling. The results obtained demonstrate the potential of IL-17 not only to inhibit the myogenic differentiation of C2C12 myoblasts but also to convert their differentiation pathway into that of osteoblast lineage providing new insight into the capacities of IL-17 to modulate the differentiation commitment.
PB - Elsevier, Amsterdam
T2 - Biochimica et Biophysica Acta-Molecular Cell Research
T1 - Interleukin 17 inhibits myogenic and promotes osteogenic differentiation of C2C12 myoblasts by activating ERK1,2
EP - 849
IS - 4
SP - 838
VL - 1823
DO - 10.1016/j.bbamcr.2012.01.001
ER -
@article{
author = "Krstić, Jelena and Santibanez, Juan and Krstić, Aleksandra and Mojsilović, Slavko and Okić-Đorđević, Ivana and Trivanović, Drenka and Ilić, Vesna and Bugarski, Diana",
year = "2012",
abstract = "The present study evaluated the role of interleukin (IL) 17 in multilineage commitment of C2C12 myoblastic cells and investigated associated signaling pathways. The results concerning the effects on cell function showed that IL-17 inhibits the migration of C2C12 cells, while not affecting their proliferation. The data regarding the influence on differentiation demonstrated that IL-17 inhibits myogenic differentiation of C2C12 cells by down-regulating the myogenin mRNA level, myosin heavy chain expression and myotube formation, but promotes their osteogenic differentiation by up-regulating the Runt-related transcription factor 2 mRNA level, cyclooxygenase-2 expression and alkaline phosphatase activity. IL-17 exerted these effects by activating ERK1,2 mitogen activated protein kinase signaling pathway, which in turn regulated the expression of relevant genes and proteins to inhibit myogenic differentiation and induce osteogenic differentiation. Additional analysis showed that the induction of osteogenic differentiation by IL-17 is independent of BMP signaling. The results obtained demonstrate the potential of IL-17 not only to inhibit the myogenic differentiation of C2C12 myoblasts but also to convert their differentiation pathway into that of osteoblast lineage providing new insight into the capacities of IL-17 to modulate the differentiation commitment.",
publisher = "Elsevier, Amsterdam",
journal = "Biochimica et Biophysica Acta-Molecular Cell Research",
title = "Interleukin 17 inhibits myogenic and promotes osteogenic differentiation of C2C12 myoblasts by activating ERK1,2",
pages = "849-838",
number = "4",
volume = "1823",
doi = "10.1016/j.bbamcr.2012.01.001"
}
Krstić, J., Santibanez, J., Krstić, A., Mojsilović, S., Okić-Đorđević, I., Trivanović, D., Ilić, V.,& Bugarski, D.. (2012). Interleukin 17 inhibits myogenic and promotes osteogenic differentiation of C2C12 myoblasts by activating ERK1,2. in Biochimica et Biophysica Acta-Molecular Cell Research
Elsevier, Amsterdam., 1823(4), 838-849.
https://doi.org/10.1016/j.bbamcr.2012.01.001
conv_2729
Krstić J, Santibanez J, Krstić A, Mojsilović S, Okić-Đorđević I, Trivanović D, Ilić V, Bugarski D. Interleukin 17 inhibits myogenic and promotes osteogenic differentiation of C2C12 myoblasts by activating ERK1,2. in Biochimica et Biophysica Acta-Molecular Cell Research. 2012;1823(4):838-849.
doi:10.1016/j.bbamcr.2012.01.001
conv_2729 .
Krstić, Jelena, Santibanez, Juan, Krstić, Aleksandra, Mojsilović, Slavko, Okić-Đorđević, Ivana, Trivanović, Drenka, Ilić, Vesna, Bugarski, Diana, "Interleukin 17 inhibits myogenic and promotes osteogenic differentiation of C2C12 myoblasts by activating ERK1,2" in Biochimica et Biophysica Acta-Molecular Cell Research, 1823, no. 4 (2012):838-849,
https://doi.org/10.1016/j.bbamcr.2012.01.001 .,
conv_2729 .
