The Acute Effect of Ethanol on Adrenal Cortex in Female Rats-Possible Role of Nitric Oxide

2011
Authors
Đikić, DragoslavaBudeč, Mirela

Vranješ-Đurić, Sanja

Koko, Vesna
Vignjević, Sanja

Mitrović, Olivera

Article (Published version)

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Aims: The present study was designed to investigate a possible role of endogenous nitric oxide (NO) in the adrenal response to an acute alcohol administration in female rats. To this end, N(omega)-nitro-l-arginine-methyl ester (l-NAME), a competitive inhibitor of all isoforms of NO synthase, was used. Methods: Adult female Wistar rats showing diestrus Day 1 were treated with: (a) ethanol (2 or 4 g/kg, intraperitoneally); (b) l-NAME (30 or 50 mg/kg, subcutaneously) followed by either ethanol or saline 3 h later. Untreated and saline-injected rats were used as controls. The animals were killed 30 min after last injection. Adrenal cortex was analyzed morphometrically, and plasma levels of adrenocorticotropic hormone (ACTH) and serum concentrations of corticosterone were determined. Results: Acute ethanol treatment enhanced the levels of ACTH and corticosterone in a dose-dependent manner. Stereological analysis revealed that acute alcohol administration induced a significant increase in ab...solute volume of the cortex and the zona fasciculata (ZF). In addition, ethanol at a dose of 4 g/kg increased volume density and length of the capillaries in the ZF. However, other stereological parameters were unaffected by alcohol exposure. Pretreatment with both doses of l-NAME had no effect on ethanol-induced changes. Conclusion: Obtained findings indicate that acute ethanol treatment stimulates the activity of the adrenal cortex and that this effect is not mediated by endogenous NO in female rats under these experimental conditions.
Source:
Alcohol & Alcoholism, 2011, 46, 5, 523-528Publisher:
- Oxford Univ Press, Oxford
Funding / projects:
DOI: 10.1093/alcalc/agr054
ISSN: 0735-0414
PubMed: 21610084
WoS: 000294066300003
Scopus: 2-s2.0-80051978015
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Institut za medicinska istraživanjaTY - JOUR AU - Đikić, Dragoslava AU - Budeč, Mirela AU - Vranješ-Đurić, Sanja AU - Koko, Vesna AU - Vignjević, Sanja AU - Mitrović, Olivera PY - 2011 UR - http://rimi.imi.bg.ac.rs/handle/123456789/335 AB - Aims: The present study was designed to investigate a possible role of endogenous nitric oxide (NO) in the adrenal response to an acute alcohol administration in female rats. To this end, N(omega)-nitro-l-arginine-methyl ester (l-NAME), a competitive inhibitor of all isoforms of NO synthase, was used. Methods: Adult female Wistar rats showing diestrus Day 1 were treated with: (a) ethanol (2 or 4 g/kg, intraperitoneally); (b) l-NAME (30 or 50 mg/kg, subcutaneously) followed by either ethanol or saline 3 h later. Untreated and saline-injected rats were used as controls. The animals were killed 30 min after last injection. Adrenal cortex was analyzed morphometrically, and plasma levels of adrenocorticotropic hormone (ACTH) and serum concentrations of corticosterone were determined. Results: Acute ethanol treatment enhanced the levels of ACTH and corticosterone in a dose-dependent manner. Stereological analysis revealed that acute alcohol administration induced a significant increase in absolute volume of the cortex and the zona fasciculata (ZF). In addition, ethanol at a dose of 4 g/kg increased volume density and length of the capillaries in the ZF. However, other stereological parameters were unaffected by alcohol exposure. Pretreatment with both doses of l-NAME had no effect on ethanol-induced changes. Conclusion: Obtained findings indicate that acute ethanol treatment stimulates the activity of the adrenal cortex and that this effect is not mediated by endogenous NO in female rats under these experimental conditions. PB - Oxford Univ Press, Oxford T2 - Alcohol & Alcoholism T1 - The Acute Effect of Ethanol on Adrenal Cortex in Female Rats-Possible Role of Nitric Oxide EP - 528 IS - 5 SP - 523 VL - 46 DO - 10.1093/alcalc/agr054 ER -
@article{ author = "Đikić, Dragoslava and Budeč, Mirela and Vranješ-Đurić, Sanja and Koko, Vesna and Vignjević, Sanja and Mitrović, Olivera", year = "2011", abstract = "Aims: The present study was designed to investigate a possible role of endogenous nitric oxide (NO) in the adrenal response to an acute alcohol administration in female rats. To this end, N(omega)-nitro-l-arginine-methyl ester (l-NAME), a competitive inhibitor of all isoforms of NO synthase, was used. Methods: Adult female Wistar rats showing diestrus Day 1 were treated with: (a) ethanol (2 or 4 g/kg, intraperitoneally); (b) l-NAME (30 or 50 mg/kg, subcutaneously) followed by either ethanol or saline 3 h later. Untreated and saline-injected rats were used as controls. The animals were killed 30 min after last injection. Adrenal cortex was analyzed morphometrically, and plasma levels of adrenocorticotropic hormone (ACTH) and serum concentrations of corticosterone were determined. Results: Acute ethanol treatment enhanced the levels of ACTH and corticosterone in a dose-dependent manner. Stereological analysis revealed that acute alcohol administration induced a significant increase in absolute volume of the cortex and the zona fasciculata (ZF). In addition, ethanol at a dose of 4 g/kg increased volume density and length of the capillaries in the ZF. However, other stereological parameters were unaffected by alcohol exposure. Pretreatment with both doses of l-NAME had no effect on ethanol-induced changes. Conclusion: Obtained findings indicate that acute ethanol treatment stimulates the activity of the adrenal cortex and that this effect is not mediated by endogenous NO in female rats under these experimental conditions.", publisher = "Oxford Univ Press, Oxford", journal = "Alcohol & Alcoholism", title = "The Acute Effect of Ethanol on Adrenal Cortex in Female Rats-Possible Role of Nitric Oxide", pages = "528-523", number = "5", volume = "46", doi = "10.1093/alcalc/agr054" }
Đikić, D., Budeč, M., Vranješ-Đurić, S., Koko, V., Vignjević, S.,& Mitrović, O.. (2011). The Acute Effect of Ethanol on Adrenal Cortex in Female Rats-Possible Role of Nitric Oxide. in Alcohol & Alcoholism Oxford Univ Press, Oxford., 46(5), 523-528. https://doi.org/10.1093/alcalc/agr054
Đikić D, Budeč M, Vranješ-Đurić S, Koko V, Vignjević S, Mitrović O. The Acute Effect of Ethanol on Adrenal Cortex in Female Rats-Possible Role of Nitric Oxide. in Alcohol & Alcoholism. 2011;46(5):523-528. doi:10.1093/alcalc/agr054 .
Đikić, Dragoslava, Budeč, Mirela, Vranješ-Đurić, Sanja, Koko, Vesna, Vignjević, Sanja, Mitrović, Olivera, "The Acute Effect of Ethanol on Adrenal Cortex in Female Rats-Possible Role of Nitric Oxide" in Alcohol & Alcoholism, 46, no. 5 (2011):523-528, https://doi.org/10.1093/alcalc/agr054 . .