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ROS-NF kappa I' mediates TGF-beta 1-induced expression of urokinase-type plasminogen activator, matrix metalloproteinase-9 and cell invasion
dc.creator | Tobar, Nicolas | |
dc.creator | Villar, Victor | |
dc.creator | Santibanez, Juan F. | |
dc.date.accessioned | 2021-04-20T12:21:13Z | |
dc.date.available | 2021-04-20T12:21:13Z | |
dc.date.issued | 2010 | |
dc.identifier.issn | 0300-8177 | |
dc.identifier.uri | http://rimi.imi.bg.ac.rs/handle/123456789/295 | |
dc.description.abstract | TGF-beta 1 has been postulated as a pro-oncogenic factor in the late step of the tumoral progression. In transformed cells, TGF-beta 1 enhances the capacity to degrade the extracellular matrix, cell invasiveness and epithelial-mesenchymal transition, which are crucial steps for metastasis. Urokinase-type plasminogen activator (uPA) and matrix metalloproteinase-9 (MMP-9) are critical components in cell migration and invasion induced by TGF-beta 1, however, the exact mechanism by which TGF-beta 1 regulates uPA and MMP-9 is not well elucidated so far. In the present study, we analyzed the role of ROS-NF kappa I', signal as mediator in the cell malignity enhancement by TGF-beta 1. We found that TGF-beta 1 activates NF kappa I', through Rac1-NOXs-ROS-dependent mechanism. Our results shows that TGF-beta 1 stimulation of uPA and MMP-9 expression involve NOXs-dependent ROS and NF kappa I', activation, demonstrated by using DPI, NOXs inhibitor, ROS scavenger N-acetylcysteine and SN50, an NFkb inhibitor. Furthermore, we found that the inhibition of ROS and NF kappa I', abrogates TGF-beta 1 stimulation of EMT, cell motility and invasion. Thus, ROS-NF kappa I' acts as the crucial signal in TGF-beta 1-induced uPA and MMP-9 expression thereby mediating the enhancement of cellular malignity by TGF-beta 1. | en |
dc.publisher | Springer, Dordrecht | |
dc.relation | Fondo Nacional de Ciencia y Tecnologia (FONDECYT), grants 1050476 and 300045 | |
dc.rights | restrictedAccess | |
dc.source | Molecular & Cellular Biochemistry | |
dc.subject | TGF-beta | en |
dc.subject | NF kappa B | en |
dc.subject | uPA | en |
dc.subject | MMP-9 | en |
dc.subject | EMT | en |
dc.subject | Migration | en |
dc.subject | Invasion | en |
dc.title | ROS-NF kappa I' mediates TGF-beta 1-induced expression of urokinase-type plasminogen activator, matrix metalloproteinase-9 and cell invasion | en |
dc.type | article | |
dc.rights.license | ARR | |
dc.citation.epage | 202 | |
dc.citation.issue | 1-2 | |
dc.citation.other | 340(1-2): 195-202 | |
dc.citation.rank | M23 | |
dc.citation.spage | 195 | |
dc.citation.volume | 340 | |
dc.identifier.doi | 10.1007/s11010-010-0418-5 | |
dc.identifier.pmid | 20204677 | |
dc.identifier.scopus | 2-s2.0-77954428063 | |
dc.identifier.wos | 000278742300025 | |
dc.type.version | publishedVersion |