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Combined effect of IL-17 and blockade of nitric oxide biosynthesis on haematopoiesis in mice

Authorized Users Only
2010
Authors
Krstić, Aleksandra
Santibanez, Juan
Okić, Ivana
Mojsilović, S.
Kocić, Jelena
Jovčić, Gordana
Milenković, P.
Bugarski, Diana
Article (Published version)
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Abstract
Aim: The study was undertaken to extend our investigation concerning both the in vivo activity of interleukin (IL)-17 and the specific role of nitric oxide (NO) in IL-17-induced effects in the process of haematopoiesis. Methods: CBA mice were simultaneously treated with IL-17 and/or nitric oxide synthase (NOS) inhibitor, l-NAME, for 5 days and changes within various haematopoietic cell lineages in bone marrow, spleen and peripheral blood were analysed. Results: Findings showed that administration of both IL-17 and l-NAME stimulated increase in net haematopoiesis in normal mice. IL-17-enhanced myelopoiesis was characterized by stimulation of both femoral and splenic haematopoietic progenitor cells and morphologically recognizable granulocytes. Additionally, IL-17 induced alterations in the frequency of erythroid progenitor cells in both bone marrow and spleen, accompanied with their mobilization to the peripheral blood. As a consequence of these changes in the erythroid cell compartment...s, significant reticulocytosis was observed, which evidenced that in IL-17-treated mice effective erythropoiesis occurred. Exposure of mice to NOS inhibitor also increased the number of both granulocyte-macrophage and erythroid progenitors in bone marrow and spleens, and these alterations were followed by the mobilization of erythroid progenitors and elevated content of reticulocytes in peripheral blood. The specific role of NO in IL-17-induced haematopoiesis was demonstrated only in the IL-17-reducing effect on bone marrow late stage erythroid progenitors, CFU-E. Conclusion: The results demonstrated the involvement of both IL-17 and NO in the regulation of haematopoietic cell activity in various haematopoietic compartments. They further suggest that IL-17 effects are differentially mediated depending on the haematopoietic microenvironments.

Keywords:
bone marrow / haematopoietic progenitors / IL-17 / NOS / spleen
Source:
Acta Physiologica, 2010, 199, 1, 31-41
Publisher:
  • Wiley, Hoboken
Funding / projects:
  • Ćelijski i molekularni mehanizmi regilacije hematopoeze (RS-145048)

DOI: 10.1111/j.1748-1716.2010.02082.x

ISSN: 1748-1708

PubMed: 20102341

WoS: 000276601600004

Scopus: 2-s2.0-77950829377
[ Google Scholar ]
10
8
URI
http://rimi.imi.bg.ac.rs/handle/123456789/266
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
Institut za medicinska istraživanja
TY  - JOUR
AU  - Krstić, Aleksandra
AU  - Santibanez, Juan
AU  - Okić, Ivana
AU  - Mojsilović, S.
AU  - Kocić, Jelena
AU  - Jovčić, Gordana
AU  - Milenković, P.
AU  - Bugarski, Diana
PY  - 2010
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/266
AB  - Aim: The study was undertaken to extend our investigation concerning both the in vivo activity of interleukin (IL)-17 and the specific role of nitric oxide (NO) in IL-17-induced effects in the process of haematopoiesis. Methods: CBA mice were simultaneously treated with IL-17 and/or nitric oxide synthase (NOS) inhibitor, l-NAME, for 5 days and changes within various haematopoietic cell lineages in bone marrow, spleen and peripheral blood were analysed. Results: Findings showed that administration of both IL-17 and l-NAME stimulated increase in net haematopoiesis in normal mice. IL-17-enhanced myelopoiesis was characterized by stimulation of both femoral and splenic haematopoietic progenitor cells and morphologically recognizable granulocytes. Additionally, IL-17 induced alterations in the frequency of erythroid progenitor cells in both bone marrow and spleen, accompanied with their mobilization to the peripheral blood. As a consequence of these changes in the erythroid cell compartments, significant reticulocytosis was observed, which evidenced that in IL-17-treated mice effective erythropoiesis occurred. Exposure of mice to NOS inhibitor also increased the number of both granulocyte-macrophage and erythroid progenitors in bone marrow and spleens, and these alterations were followed by the mobilization of erythroid progenitors and elevated content of reticulocytes in peripheral blood. The specific role of NO in IL-17-induced haematopoiesis was demonstrated only in the IL-17-reducing effect on bone marrow late stage erythroid progenitors, CFU-E. Conclusion: The results demonstrated the involvement of both IL-17 and NO in the regulation of haematopoietic cell activity in various haematopoietic compartments. They further suggest that IL-17 effects are differentially mediated depending on the haematopoietic microenvironments.
PB  - Wiley, Hoboken
T2  - Acta Physiologica
T1  - Combined effect of IL-17 and blockade of nitric oxide biosynthesis on haematopoiesis in mice
EP  - 41
IS  - 1
SP  - 31
VL  - 199
DO  - 10.1111/j.1748-1716.2010.02082.x
UR  - conv_2272
ER  - 
@article{
author = "Krstić, Aleksandra and Santibanez, Juan and Okić, Ivana and Mojsilović, S. and Kocić, Jelena and Jovčić, Gordana and Milenković, P. and Bugarski, Diana",
year = "2010",
abstract = "Aim: The study was undertaken to extend our investigation concerning both the in vivo activity of interleukin (IL)-17 and the specific role of nitric oxide (NO) in IL-17-induced effects in the process of haematopoiesis. Methods: CBA mice were simultaneously treated with IL-17 and/or nitric oxide synthase (NOS) inhibitor, l-NAME, for 5 days and changes within various haematopoietic cell lineages in bone marrow, spleen and peripheral blood were analysed. Results: Findings showed that administration of both IL-17 and l-NAME stimulated increase in net haematopoiesis in normal mice. IL-17-enhanced myelopoiesis was characterized by stimulation of both femoral and splenic haematopoietic progenitor cells and morphologically recognizable granulocytes. Additionally, IL-17 induced alterations in the frequency of erythroid progenitor cells in both bone marrow and spleen, accompanied with their mobilization to the peripheral blood. As a consequence of these changes in the erythroid cell compartments, significant reticulocytosis was observed, which evidenced that in IL-17-treated mice effective erythropoiesis occurred. Exposure of mice to NOS inhibitor also increased the number of both granulocyte-macrophage and erythroid progenitors in bone marrow and spleens, and these alterations were followed by the mobilization of erythroid progenitors and elevated content of reticulocytes in peripheral blood. The specific role of NO in IL-17-induced haematopoiesis was demonstrated only in the IL-17-reducing effect on bone marrow late stage erythroid progenitors, CFU-E. Conclusion: The results demonstrated the involvement of both IL-17 and NO in the regulation of haematopoietic cell activity in various haematopoietic compartments. They further suggest that IL-17 effects are differentially mediated depending on the haematopoietic microenvironments.",
publisher = "Wiley, Hoboken",
journal = "Acta Physiologica",
title = "Combined effect of IL-17 and blockade of nitric oxide biosynthesis on haematopoiesis in mice",
pages = "41-31",
number = "1",
volume = "199",
doi = "10.1111/j.1748-1716.2010.02082.x",
url = "conv_2272"
}
Krstić, A., Santibanez, J., Okić, I., Mojsilović, S., Kocić, J., Jovčić, G., Milenković, P.,& Bugarski, D.. (2010). Combined effect of IL-17 and blockade of nitric oxide biosynthesis on haematopoiesis in mice. in Acta Physiologica
Wiley, Hoboken., 199(1), 31-41.
https://doi.org/10.1111/j.1748-1716.2010.02082.x
conv_2272
Krstić A, Santibanez J, Okić I, Mojsilović S, Kocić J, Jovčić G, Milenković P, Bugarski D. Combined effect of IL-17 and blockade of nitric oxide biosynthesis on haematopoiesis in mice. in Acta Physiologica. 2010;199(1):31-41.
doi:10.1111/j.1748-1716.2010.02082.x
conv_2272 .
Krstić, Aleksandra, Santibanez, Juan, Okić, Ivana, Mojsilović, S., Kocić, Jelena, Jovčić, Gordana, Milenković, P., Bugarski, Diana, "Combined effect of IL-17 and blockade of nitric oxide biosynthesis on haematopoiesis in mice" in Acta Physiologica, 199, no. 1 (2010):31-41,
https://doi.org/10.1111/j.1748-1716.2010.02082.x .,
conv_2272 .

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