The multiple effects of interleukin-1 (IL-1) on hematopoietic cells are mainly documented in disturbed hematopoiesis, but its production and participation during constitutive hematopoiesis are still unproven, To assess the involvement of IL-1 in the regulation of steady-state hematopoiesis in vivo, we have investigated the consequences of IL-1 receptor blockade by recombinant human IL-1 receptor antagonist (rhIL-1Ra) in normal CBA/H mice treated with two i.p. injections of rhIL-1Ra (2 x 50 mu g/mouse) seventeen and two hours before sacrifice. The cellularity, the number of granulocyte-macrophage (CFU-GM), the number of erythroid (BFU-E) progenitor cells and the percentage of these cells in S phase of the cell cycle, as well as the morphologically recognizable cells in bone marrow were estimated, In peripheral blood, hematocrit, the number and differential count of nucleated cells, the number of erythrocytes and the percentage of reticulocytes were determined, IL-1Ra treatment significa...ntly reduced the number of femoral CFU-GM and BFU-E, while all the other analyzed parameters were not different from the level obtained in control, non-treated animals. These findings show that a number of bone marrow IL-l-responsive cells were affected by the IL-1 receptor blockade, indicating that the expression of IL-1 receptors and endogenous IL-1 secretion occur as part of constitutive hematopoiesis.
TY - JOUR
AU - Jovčić, Gordana
AU - Ivanović, Z
AU - Biljanovic-Paunović, L
AU - Bugarski, Diana
AU - Stošić-Grujičić, Stanislava
AU - Milenković, P.
PY - 1996
UR - http://rimi.imi.bg.ac.rs/handle/123456789/25
AB - The multiple effects of interleukin-1 (IL-1) on hematopoietic cells are mainly documented in disturbed hematopoiesis, but its production and participation during constitutive hematopoiesis are still unproven, To assess the involvement of IL-1 in the regulation of steady-state hematopoiesis in vivo, we have investigated the consequences of IL-1 receptor blockade by recombinant human IL-1 receptor antagonist (rhIL-1Ra) in normal CBA/H mice treated with two i.p. injections of rhIL-1Ra (2 x 50 mu g/mouse) seventeen and two hours before sacrifice. The cellularity, the number of granulocyte-macrophage (CFU-GM), the number of erythroid (BFU-E) progenitor cells and the percentage of these cells in S phase of the cell cycle, as well as the morphologically recognizable cells in bone marrow were estimated, In peripheral blood, hematocrit, the number and differential count of nucleated cells, the number of erythrocytes and the percentage of reticulocytes were determined, IL-1Ra treatment significantly reduced the number of femoral CFU-GM and BFU-E, while all the other analyzed parameters were not different from the level obtained in control, non-treated animals. These findings show that a number of bone marrow IL-l-responsive cells were affected by the IL-1 receptor blockade, indicating that the expression of IL-1 receptors and endogenous IL-1 secretion occur as part of constitutive hematopoiesis.
PB - John Libbey Eurotext Ltd, Montrouge
T2 - European Cytokine Network
T1 - In vivo effects of interleukin-1 receptor antagonist on hematopoietic bone marrow progenitor cells in normal mice
EP - 74
IS - 1
SP - 71
VL - 7
UR - conv_4993
ER -
@article{
author = "Jovčić, Gordana and Ivanović, Z and Biljanovic-Paunović, L and Bugarski, Diana and Stošić-Grujičić, Stanislava and Milenković, P.",
year = "1996",
abstract = "The multiple effects of interleukin-1 (IL-1) on hematopoietic cells are mainly documented in disturbed hematopoiesis, but its production and participation during constitutive hematopoiesis are still unproven, To assess the involvement of IL-1 in the regulation of steady-state hematopoiesis in vivo, we have investigated the consequences of IL-1 receptor blockade by recombinant human IL-1 receptor antagonist (rhIL-1Ra) in normal CBA/H mice treated with two i.p. injections of rhIL-1Ra (2 x 50 mu g/mouse) seventeen and two hours before sacrifice. The cellularity, the number of granulocyte-macrophage (CFU-GM), the number of erythroid (BFU-E) progenitor cells and the percentage of these cells in S phase of the cell cycle, as well as the morphologically recognizable cells in bone marrow were estimated, In peripheral blood, hematocrit, the number and differential count of nucleated cells, the number of erythrocytes and the percentage of reticulocytes were determined, IL-1Ra treatment significantly reduced the number of femoral CFU-GM and BFU-E, while all the other analyzed parameters were not different from the level obtained in control, non-treated animals. These findings show that a number of bone marrow IL-l-responsive cells were affected by the IL-1 receptor blockade, indicating that the expression of IL-1 receptors and endogenous IL-1 secretion occur as part of constitutive hematopoiesis.",
publisher = "John Libbey Eurotext Ltd, Montrouge",
journal = "European Cytokine Network",
title = "In vivo effects of interleukin-1 receptor antagonist on hematopoietic bone marrow progenitor cells in normal mice",
pages = "74-71",
number = "1",
volume = "7",
url = "conv_4993"
}
Jovčić, G., Ivanović, Z., Biljanovic-Paunović, L., Bugarski, D., Stošić-Grujičić, S.,& Milenković, P.. (1996). In vivo effects of interleukin-1 receptor antagonist on hematopoietic bone marrow progenitor cells in normal mice. in European Cytokine Network
John Libbey Eurotext Ltd, Montrouge., 7(1), 71-74.
conv_4993
Jovčić G, Ivanović Z, Biljanovic-Paunović L, Bugarski D, Stošić-Grujičić S, Milenković P. In vivo effects of interleukin-1 receptor antagonist on hematopoietic bone marrow progenitor cells in normal mice. in European Cytokine Network. 1996;7(1):71-74.
conv_4993 .
Jovčić, Gordana, Ivanović, Z, Biljanovic-Paunović, L, Bugarski, Diana, Stošić-Grujičić, Stanislava, Milenković, P., "In vivo effects of interleukin-1 receptor antagonist on hematopoietic bone marrow progenitor cells in normal mice" in European Cytokine Network, 7, no. 1 (1996):71-74,
conv_4993 .
