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Low O-2 concentrations enhance the positive effect of IL-17 on the maintenance of erythroid progenitors during co-culture of CD34+and mesenchymal stem cells
dc.creator | Krstić, Aleksandra | |
dc.creator | Vlaški, Marija | |
dc.creator | Hammoud, Mohammad | |
dc.creator | Chevaleyre, Jean | |
dc.creator | Duchez, Pascale | |
dc.creator | Jovčić, Gordana | |
dc.creator | Bugarski, Diana | |
dc.creator | Milenković, Pavle B. | |
dc.creator | Bourin, Philippe | |
dc.creator | Boiron, Jean-Michel | |
dc.creator | Praloran, Vincent | |
dc.creator | Ivanović, Zoran | |
dc.date.accessioned | 2021-04-20T12:17:49Z | |
dc.date.available | 2021-04-20T12:17:49Z | |
dc.date.issued | 2009 | |
dc.identifier.issn | 1952-4005 | |
dc.identifier.uri | http://rimi.imi.bg.ac.rs/handle/123456789/245 | |
dc.description.abstract | Co-culture of haematopoietic cells with a stromal cell layer does not mimic the physiological, micro-environmental niche, whose major feature is a low oxygen (O-2) concentration. Thus, in order to study the effects of IL-17 in a context which better approximates the physiological state, we investigated its effects on cell expansion, colony-forming ability, and the phenotypical profile of normal, human blood CD34(+) cells co-cultured for five days with MSC layers at various O-2 concentrations (20%, 12.5% and 3% O-2). We demonstrated that IL-17 enhances CD34(+) and total CFC production during the five days of MSC/CD34(+) co-culture. This effect depends upon the O-2 concentration, reaching its maximum at 3% O-2, and is more pronounced on erythroid progenitors (BFU-E). In addition, the stimulation of IL-6 production by IL-17 in MSC cultures and co-cultures is enhanced by low O-2 concentration. The expression of some differentiation markers (CD34, CD13 and CD41) on haematopoietic cells in co-cultures also depends upon the oxygen concentration. Our results strengthen the concept that physiological levels of O-2 (mistakenly called hypoxia), should be considered as an important environmental factor that significantly influences cytokine activity. | en |
dc.publisher | John Libbey Eurotext Ltd, Montrouge | |
dc.relation | Scientific Council of the French Blood Institute (EFS) [CS-2005.05] | |
dc.relation | National Institute for Blood Transfusion (INTS) | |
dc.relation | info:eu-repo/grantAgreement/MESTD/MPN2006-2010/145048/RS// | |
dc.relation | Ligue Nationale Francaise Contre le Cancer (Comite Aquitain) | |
dc.relation | Ministry of Education of the Syrian Government | |
dc.rights | restrictedAccess | |
dc.source | European Cytokine Network | |
dc.subject | hematopoietic progenitors | en |
dc.subject | CD34+ | en |
dc.subject | IL-17 | en |
dc.subject | oxygen | en |
dc.subject | hypoxia | en |
dc.title | Low O-2 concentrations enhance the positive effect of IL-17 on the maintenance of erythroid progenitors during co-culture of CD34+and mesenchymal stem cells | en |
dc.type | article | |
dc.rights.license | ARR | |
dc.citation.epage | 16 | |
dc.citation.issue | 1 | |
dc.citation.other | 20(1): 10-16 | |
dc.citation.spage | 10 | |
dc.citation.volume | 20 | |
dc.identifier.doi | 10.1684/ecn.2009.0145 | |
dc.identifier.pmid | 19318315 | |
dc.identifier.scopus | 2-s2.0-66249087948 | |
dc.identifier.wos | 000264811600003 | |
dc.type.version | publishedVersion |