Objective: Nitric oxide (NO) is known as a regulator of inflammation and immunity. The purpose of this study was to investigate the influence of this signal molecule on the rat immunoglobulin A (IgA) system using N omega-nitro-L-arginine-methyl ester (L-NAME), which inhibits the activity of all isoforms of NO synthase. Methods: The experiments were performed on adult female Wistar rats showing diestrus day 1 that were treated with L-NAME (30 or 50 mg/kg, s.c.). Untreated and saline-injected animals were used as controls. The rats were sacrificed 3 h following L-NAME or saline administration. The concentration of IgA in serum and intestinal extracts was determined by a sandwich enzyme-linked immunosorbent assay. The number of IgA-expressing cells per area unit of Peyer's patches and the intestinal lamina propria was evaluated using stereological analysis. Results: The results showed that L-NAME decreased the level of IgA in serum and elevated its concentration in intestinal extracts. Ad...ditionally, the increased number of IgA+ cells was found in the intestinal lamina propria in both experimental groups. Conclusion: Obtained findings imply that endogenous NO may modulate the IgA system in the rat. Copyright (C) 2009 S. Karger AG, Basel
Keywords:
N omega-nitro-L-arginine-methyl ester / Immunoglobulin A / Rat small intestine
TY - JOUR
AU - Budeč, Mirela
AU - Marković, Dragana
AU - Đikić, Dragoslava
AU - Mitrović, Olivera
AU - Drndarević, Neda
AU - Koko, Vesna
AU - Todorović, Vera
PY - 2009
UR - http://rimi.imi.bg.ac.rs/handle/123456789/219
AB - Objective: Nitric oxide (NO) is known as a regulator of inflammation and immunity. The purpose of this study was to investigate the influence of this signal molecule on the rat immunoglobulin A (IgA) system using N omega-nitro-L-arginine-methyl ester (L-NAME), which inhibits the activity of all isoforms of NO synthase. Methods: The experiments were performed on adult female Wistar rats showing diestrus day 1 that were treated with L-NAME (30 or 50 mg/kg, s.c.). Untreated and saline-injected animals were used as controls. The rats were sacrificed 3 h following L-NAME or saline administration. The concentration of IgA in serum and intestinal extracts was determined by a sandwich enzyme-linked immunosorbent assay. The number of IgA-expressing cells per area unit of Peyer's patches and the intestinal lamina propria was evaluated using stereological analysis. Results: The results showed that L-NAME decreased the level of IgA in serum and elevated its concentration in intestinal extracts. Additionally, the increased number of IgA+ cells was found in the intestinal lamina propria in both experimental groups. Conclusion: Obtained findings imply that endogenous NO may modulate the IgA system in the rat. Copyright (C) 2009 S. Karger AG, Basel
PB - Karger, Basel
T2 - Neuroimmunomodulation
T1 - Blockade of Nitric Oxide Synthesis Modulates Rat Immunoglobulin A
EP - 161
IS - 3
SP - 155
VL - 16
DO - 10.1159/000204228
UR - conv_2064
ER -
@article{
author = "Budeč, Mirela and Marković, Dragana and Đikić, Dragoslava and Mitrović, Olivera and Drndarević, Neda and Koko, Vesna and Todorović, Vera",
year = "2009",
abstract = "Objective: Nitric oxide (NO) is known as a regulator of inflammation and immunity. The purpose of this study was to investigate the influence of this signal molecule on the rat immunoglobulin A (IgA) system using N omega-nitro-L-arginine-methyl ester (L-NAME), which inhibits the activity of all isoforms of NO synthase. Methods: The experiments were performed on adult female Wistar rats showing diestrus day 1 that were treated with L-NAME (30 or 50 mg/kg, s.c.). Untreated and saline-injected animals were used as controls. The rats were sacrificed 3 h following L-NAME or saline administration. The concentration of IgA in serum and intestinal extracts was determined by a sandwich enzyme-linked immunosorbent assay. The number of IgA-expressing cells per area unit of Peyer's patches and the intestinal lamina propria was evaluated using stereological analysis. Results: The results showed that L-NAME decreased the level of IgA in serum and elevated its concentration in intestinal extracts. Additionally, the increased number of IgA+ cells was found in the intestinal lamina propria in both experimental groups. Conclusion: Obtained findings imply that endogenous NO may modulate the IgA system in the rat. Copyright (C) 2009 S. Karger AG, Basel",
publisher = "Karger, Basel",
journal = "Neuroimmunomodulation",
title = "Blockade of Nitric Oxide Synthesis Modulates Rat Immunoglobulin A",
pages = "161-155",
number = "3",
volume = "16",
doi = "10.1159/000204228",
url = "conv_2064"
}
Budeč, M., Marković, D., Đikić, D., Mitrović, O., Drndarević, N., Koko, V.,& Todorović, V.. (2009). Blockade of Nitric Oxide Synthesis Modulates Rat Immunoglobulin A. in Neuroimmunomodulation
Karger, Basel., 16(3), 155-161.
https://doi.org/10.1159/000204228
conv_2064
Budeč M, Marković D, Đikić D, Mitrović O, Drndarević N, Koko V, Todorović V. Blockade of Nitric Oxide Synthesis Modulates Rat Immunoglobulin A. in Neuroimmunomodulation. 2009;16(3):155-161.
doi:10.1159/000204228
conv_2064 .
