Growth factors play an important role in orchestrating and enabling the cellular responses required for successful wound healing. In the present study, rat surgical incision was used to investigate insulin-like growth factor-I (IGF-I) expression in skin cells as well as its systemic and cutaneous tissue concentrations during acute phase of wound healing. Thirty two animals were sacrificed at days 2, 3, 5 and 9 after surgery. Eight animals were used as control. Tissue expression of IGF-I in both incisional and periincisional skin areas, as well as in skin of control unwounded animals was determined by immunohistochemistry. Serum and tissue concentrations of IGF-I were measured using RIA. Immunohistochemical analysis revealed enhanced IGF-I immunostaining in the incisional area at day 2 post-wounding. Presence of IGF-I immuno reactivity in the epidermis, as well as in dermal fibroblasts and monocytes within perivascular inflammatory infiltrate suggests its local synthesis. Although serum... levels of IGN-I were not altered during wound healing, their tissue contents in the incisional area were significantly increased compared with periincisional area at days 2 and 3 after injury, as well as compared with skin content of unwounded control rats in all examined time points. Obtained results support a paracrine role of IGN during the acute phase of wound healing by primary intention in the rat.
TY - JOUR
AU - Todorović, Vera
AU - Pesko, Predrag
AU - Micev, Marjan
AU - Bjelović, Miloš
AU - Budeč, Mirela
AU - Mićić, Mileva
AU - Brasanac, Dimitrije
AU - Ilic-Stojanović, Olivera
PY - 2008
UR - http://rimi.imi.bg.ac.rs/handle/123456789/213
AB - Growth factors play an important role in orchestrating and enabling the cellular responses required for successful wound healing. In the present study, rat surgical incision was used to investigate insulin-like growth factor-I (IGF-I) expression in skin cells as well as its systemic and cutaneous tissue concentrations during acute phase of wound healing. Thirty two animals were sacrificed at days 2, 3, 5 and 9 after surgery. Eight animals were used as control. Tissue expression of IGF-I in both incisional and periincisional skin areas, as well as in skin of control unwounded animals was determined by immunohistochemistry. Serum and tissue concentrations of IGF-I were measured using RIA. Immunohistochemical analysis revealed enhanced IGF-I immunostaining in the incisional area at day 2 post-wounding. Presence of IGF-I immuno reactivity in the epidermis, as well as in dermal fibroblasts and monocytes within perivascular inflammatory infiltrate suggests its local synthesis. Although serum levels of IGN-I were not altered during wound healing, their tissue contents in the incisional area were significantly increased compared with periincisional area at days 2 and 3 after injury, as well as compared with skin content of unwounded control rats in all examined time points. Obtained results support a paracrine role of IGN during the acute phase of wound healing by primary intention in the rat.
PB - Elsevier Science Bv, Amsterdam
T2 - Regulatory Peptides
T1 - Insulin-like growth factor-I in wound healing of rat skin
EP - 13
IS - 1-3
SP - 7
VL - 150
DO - 10.1016/j.regpep.2008.05.006
UR - conv_2011
ER -
@article{
author = "Todorović, Vera and Pesko, Predrag and Micev, Marjan and Bjelović, Miloš and Budeč, Mirela and Mićić, Mileva and Brasanac, Dimitrije and Ilic-Stojanović, Olivera",
year = "2008",
abstract = "Growth factors play an important role in orchestrating and enabling the cellular responses required for successful wound healing. In the present study, rat surgical incision was used to investigate insulin-like growth factor-I (IGF-I) expression in skin cells as well as its systemic and cutaneous tissue concentrations during acute phase of wound healing. Thirty two animals were sacrificed at days 2, 3, 5 and 9 after surgery. Eight animals were used as control. Tissue expression of IGF-I in both incisional and periincisional skin areas, as well as in skin of control unwounded animals was determined by immunohistochemistry. Serum and tissue concentrations of IGF-I were measured using RIA. Immunohistochemical analysis revealed enhanced IGF-I immunostaining in the incisional area at day 2 post-wounding. Presence of IGF-I immuno reactivity in the epidermis, as well as in dermal fibroblasts and monocytes within perivascular inflammatory infiltrate suggests its local synthesis. Although serum levels of IGN-I were not altered during wound healing, their tissue contents in the incisional area were significantly increased compared with periincisional area at days 2 and 3 after injury, as well as compared with skin content of unwounded control rats in all examined time points. Obtained results support a paracrine role of IGN during the acute phase of wound healing by primary intention in the rat.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Regulatory Peptides",
title = "Insulin-like growth factor-I in wound healing of rat skin",
pages = "13-7",
number = "1-3",
volume = "150",
doi = "10.1016/j.regpep.2008.05.006",
url = "conv_2011"
}
Todorović, V., Pesko, P., Micev, M., Bjelović, M., Budeč, M., Mićić, M., Brasanac, D.,& Ilic-Stojanović, O.. (2008). Insulin-like growth factor-I in wound healing of rat skin. in Regulatory Peptides
Elsevier Science Bv, Amsterdam., 150(1-3), 7-13.
https://doi.org/10.1016/j.regpep.2008.05.006
conv_2011
Todorović V, Pesko P, Micev M, Bjelović M, Budeč M, Mićić M, Brasanac D, Ilic-Stojanović O. Insulin-like growth factor-I in wound healing of rat skin. in Regulatory Peptides. 2008;150(1-3):7-13.
doi:10.1016/j.regpep.2008.05.006
conv_2011 .
Todorović, Vera, Pesko, Predrag, Micev, Marjan, Bjelović, Miloš, Budeč, Mirela, Mićić, Mileva, Brasanac, Dimitrije, Ilic-Stojanović, Olivera, "Insulin-like growth factor-I in wound healing of rat skin" in Regulatory Peptides, 150, no. 1-3 (2008):7-13,
https://doi.org/10.1016/j.regpep.2008.05.006 .,
conv_2011 .
