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Experimental and systems biology studies of the molecular basis for the radioresistance of prostate carcinoma cells

Authorized Users Only
2008
Authors
Niciforović, Ana
Đorđević, Jelena
Adžić, Miroslav
Vučić, Vesna M.
Mitrasinović, Petar M.
Radojcić, Marija B.
Article (Published version)
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Abstract
Molecular mechanisms for the gamma-ionizing radiation (IR) resistance of human prostate cancer cells, PC-3, are not quite clear. Since the low-LET-IR effects are primarily manifested by the generation of reactive oxygen species (ROS), the IR-induced expressions both of ROS-metabolizing antioxidant enzymes, such as Mn- and CuZn superoxide dismutases (SODs) and catalase (Cat), and of the transcriptional nuclear factor-kappaB (NF-kappa B) were explored. A substantial increase in the concentrations of SODs was observed in the cells irradiated by 10 and 20 Gy relative to those irradiated by 0 and 2 Gy, while the Cat and NF-kappa B expressions were found to be fairly stable. A system biology model was developed to shed more light on how MnSOD affects the biological state of cells depending upon the production of H2O2. By raising the initial presence of MnSOD in the 0.7-10 mu M concentration range, the time-dependent concentrations of H2O2 for various initial levels of MnSOD were contrasted. ...The radioresistance of PC-3 cells is suggested to be associated with the positive, feed-forward vicious circle established between the H2O2-mediated elevation of MnSOD expression.

Keywords:
gamma-ionizing radiation / PC-3 cells / MnSOD / CuZnSOD / radioresistance
Source:
Annals of Biomedical Engineering, 2008, 36, 5, 831-838
Publisher:
  • Springer, New York
Funding / projects:
  • Biofizička istraživanja membranskih procesa: interakcija membranskih receptora i kanala sa spoljašnjim faktorima i intracelularna regulacija (RS-143016)
  • Odgovor sisarskih ćelija na endokrini i radiobiološki stres (RS-143042)

DOI: 10.1007/s10439-008-9457-4

ISSN: 0090-6964

PubMed: 18264764

WoS: 000254755800015

Scopus: 2-s2.0-43249115087
[ Google Scholar ]
12
11
URI
http://rimi.imi.bg.ac.rs/handle/123456789/210
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
Institut za medicinska istraživanja
TY  - JOUR
AU  - Niciforović, Ana
AU  - Đorđević, Jelena
AU  - Adžić, Miroslav
AU  - Vučić, Vesna M.
AU  - Mitrasinović, Petar M.
AU  - Radojcić, Marija B.
PY  - 2008
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/210
AB  - Molecular mechanisms for the gamma-ionizing radiation (IR) resistance of human prostate cancer cells, PC-3, are not quite clear. Since the low-LET-IR effects are primarily manifested by the generation of reactive oxygen species (ROS), the IR-induced expressions both of ROS-metabolizing antioxidant enzymes, such as Mn- and CuZn superoxide dismutases (SODs) and catalase (Cat), and of the transcriptional nuclear factor-kappaB (NF-kappa B) were explored. A substantial increase in the concentrations of SODs was observed in the cells irradiated by 10 and 20 Gy relative to those irradiated by 0 and 2 Gy, while the Cat and NF-kappa B expressions were found to be fairly stable. A system biology model was developed to shed more light on how MnSOD affects the biological state of cells depending upon the production of H2O2. By raising the initial presence of MnSOD in the 0.7-10 mu M concentration range, the time-dependent concentrations of H2O2 for various initial levels of MnSOD were contrasted. The radioresistance of PC-3 cells is suggested to be associated with the positive, feed-forward vicious circle established between the H2O2-mediated elevation of MnSOD expression.
PB  - Springer, New York
T2  - Annals of Biomedical Engineering
T1  - Experimental and systems biology studies of the molecular basis for the radioresistance of prostate carcinoma cells
EP  - 838
IS  - 5
SP  - 831
VL  - 36
DO  - 10.1007/s10439-008-9457-4
UR  - conv_1923
ER  - 
@article{
author = "Niciforović, Ana and Đorđević, Jelena and Adžić, Miroslav and Vučić, Vesna M. and Mitrasinović, Petar M. and Radojcić, Marija B.",
year = "2008",
abstract = "Molecular mechanisms for the gamma-ionizing radiation (IR) resistance of human prostate cancer cells, PC-3, are not quite clear. Since the low-LET-IR effects are primarily manifested by the generation of reactive oxygen species (ROS), the IR-induced expressions both of ROS-metabolizing antioxidant enzymes, such as Mn- and CuZn superoxide dismutases (SODs) and catalase (Cat), and of the transcriptional nuclear factor-kappaB (NF-kappa B) were explored. A substantial increase in the concentrations of SODs was observed in the cells irradiated by 10 and 20 Gy relative to those irradiated by 0 and 2 Gy, while the Cat and NF-kappa B expressions were found to be fairly stable. A system biology model was developed to shed more light on how MnSOD affects the biological state of cells depending upon the production of H2O2. By raising the initial presence of MnSOD in the 0.7-10 mu M concentration range, the time-dependent concentrations of H2O2 for various initial levels of MnSOD were contrasted. The radioresistance of PC-3 cells is suggested to be associated with the positive, feed-forward vicious circle established between the H2O2-mediated elevation of MnSOD expression.",
publisher = "Springer, New York",
journal = "Annals of Biomedical Engineering",
title = "Experimental and systems biology studies of the molecular basis for the radioresistance of prostate carcinoma cells",
pages = "838-831",
number = "5",
volume = "36",
doi = "10.1007/s10439-008-9457-4",
url = "conv_1923"
}
Niciforović, A., Đorđević, J., Adžić, M., Vučić, V. M., Mitrasinović, P. M.,& Radojcić, M. B.. (2008). Experimental and systems biology studies of the molecular basis for the radioresistance of prostate carcinoma cells. in Annals of Biomedical Engineering
Springer, New York., 36(5), 831-838.
https://doi.org/10.1007/s10439-008-9457-4
conv_1923
Niciforović A, Đorđević J, Adžić M, Vučić VM, Mitrasinović PM, Radojcić MB. Experimental and systems biology studies of the molecular basis for the radioresistance of prostate carcinoma cells. in Annals of Biomedical Engineering. 2008;36(5):831-838.
doi:10.1007/s10439-008-9457-4
conv_1923 .
Niciforović, Ana, Đorđević, Jelena, Adžić, Miroslav, Vučić, Vesna M., Mitrasinović, Petar M., Radojcić, Marija B., "Experimental and systems biology studies of the molecular basis for the radioresistance of prostate carcinoma cells" in Annals of Biomedical Engineering, 36, no. 5 (2008):831-838,
https://doi.org/10.1007/s10439-008-9457-4 .,
conv_1923 .

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