Influence of dietary fats on serum phospholipid fatty acid composition and its relation to obesity in animals
Uticaj masti u obroku na masne kiseline u serumskim fosfolipidima i gojaznost životinja
2008
Аутори
Tepšić, VesnaPavlović, Mirjana A.
Ristić-Medić, Danijela
Ristić, Vanja
Lekić, N.
Tepšić, Jasna
Debeljak-Martačić, Jasmina
Milićević, Miroslav
Glibetić, Marija
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Dietary fat and its relation to obesity has been a controversial issue for many years. Experimental data shows that most, though not all animals, which consume a high fat diet, will become obese. However, the effect of fatty acids on animal obesity has not been studied in detail. In order to evaluate the effects of low versus high fat diet on serum phospholipids fatty acids composition a 4-wk study was conducted on male Wister rats. The rats were fed low-fat (10% energy) and high-fat (46% energy) foods containing constant proportions of fatty acids. Control group C was fed a standard laboratory diet (polyunsaturated/ saturated (P/S) fatty ratio 1.3), group M was fed a standard laboratory diet supplemented with margarine (P/S ratio 0.95), and the diet of the SL group was additionally supplemented with a sunflower oil-lard (1:1) mixture (P/S ratio 1.3). All lipid supplemented hyperenergetic diets caused an increase in the average daily energy intake. Both the final and the daily body wei...ght gain were significantly higher in M and SL groups than in group C. Additionally, serum triglyceride levels, LDL-cholesterol and total cholesterol were also significantly higher in M and SL groups when compared to the control group. Serum phospholipids fatty acids varied in response to total dietary fat. A significant decrease in saturated fatty acids (SFA) content (16:0 and 18:0) and an increase in monounsaturated fatty acid (MUFA) content (18:1, n-9) was found in the M group when compared to both C and SL groups. In the SL group, SFA content (18:0) was higher and MUFA content (18:1, n-9) was lower than in group C. Polyunsaturated fatty acids (PUFA) content showed an increase in both experimental groups. The PUFA/SFA ratio was higher in the M group than in the C and SL groups. Our study suggests that the amount of dietary fat has a greater influence on obesity than the effects of the type of fat consumed. However, depending on the type of fat present in the diet the differences were observed in the composition of serum PL fatty acid suggesting that both total fat and individual fatty acids have to be considered when reaching conclusions about the effect of dietary fat and obesity in animals.
Uticaj masnih kiselina u ishrani i njihov efekat na pojavu gojaznosti kod životinja je kontraverzno pitanje već dugi niz godina. Uticaj pojedinačnih masnih kiselina na pojavu gojaznosti kod životinja nije do sada detaljnije proučavan. Sa ciljem da se ispita efekat dijeta sa niskim ili visokim sadržajem masti na pojavu gojaznosti, izvršena su istraživanja na Wister pacovima u trajanju od 4 nedelje. Pacovi su hranjeni eksperimentalnim dijetama koje su sadržale standardan, nizak (10%) ili visok (46%) energetski unos poreklom od masti. Grupa kontrolnih životinja (C) je hranjena standardnom laboratorijskom dijetom (odnos polizasićenih/ zasićenih masnih kiselina/PUFA, P/S 1.3), grupa M sa standardnom dijetom sa dodatkom margarina (P/S 0.95) i grupa SL sa dijetom kojoj je dodata mešavina 1:1 suncokretovog ulja i masti (odnos P/S 1.3). Obe dijete (M i SL) su uzrokovale povećanje u prosečnom dnevnom energetskom unosu. U grupi M i SL, registrovano je značajno povećanje u telesnoj težini kontinui...rano tokom eksperimenta. Nivo serumskih triglicerida, LDL kao i ukupnog holesterola su bili značajnije povećani u M i SL grupi u poređenju sa kontrolom. Promene u profilu serumskih masnih kiselina fosfolipida su zavisile od sastava masti u eksperimentalnoj dijeti. Kod eksperimentalnih životinja grupe M primećeno je značajno smanjene serumskih zasićenih masnih kiselina (SFA, 16:0 i 18:0) kao i porast monozasićenih masnih kiselina (MUFA, 18:1, n-9) u poređenju sa SL i C grupom. Odnos PUFA/ SFA je bio veći u M u odnosu na SL i C grupu. Rezultati naših istraživanja ukazuju da količina masti u ishrani životinja ima veći uticaj na porast telesne mase nego vrsta prisutne masnoće. Promene u sastavu serumskih masnih kiselina fosfolipida u odnosu na tip masti u eksperimentalnim dijetama ukazuju na ulogu pojedinačnih masnih kiselina u procesu nastajanja gojaznosti kod životinja i te mehanizme treba dalje istraživati.
Кључне речи:
dietary fat / phospholipids fatty acids / animal obesity / animal modelИзвор:
Acta Veterinaria-Beograd, 2008, 58, 1, 33-41Издавач:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
DOI: 10.2298/AVB0801033T
ISSN: 0567-8315
WoS: 000254591100004
Scopus: 2-s2.0-43149087099
Институција/група
Institut za medicinska istraživanjaTY - JOUR AU - Tepšić, Vesna AU - Pavlović, Mirjana A. AU - Ristić-Medić, Danijela AU - Ristić, Vanja AU - Lekić, N. AU - Tepšić, Jasna AU - Debeljak-Martačić, Jasmina AU - Milićević, Miroslav AU - Glibetić, Marija PY - 2008 UR - http://rimi.imi.bg.ac.rs/handle/123456789/209 AB - Dietary fat and its relation to obesity has been a controversial issue for many years. Experimental data shows that most, though not all animals, which consume a high fat diet, will become obese. However, the effect of fatty acids on animal obesity has not been studied in detail. In order to evaluate the effects of low versus high fat diet on serum phospholipids fatty acids composition a 4-wk study was conducted on male Wister rats. The rats were fed low-fat (10% energy) and high-fat (46% energy) foods containing constant proportions of fatty acids. Control group C was fed a standard laboratory diet (polyunsaturated/ saturated (P/S) fatty ratio 1.3), group M was fed a standard laboratory diet supplemented with margarine (P/S ratio 0.95), and the diet of the SL group was additionally supplemented with a sunflower oil-lard (1:1) mixture (P/S ratio 1.3). All lipid supplemented hyperenergetic diets caused an increase in the average daily energy intake. Both the final and the daily body weight gain were significantly higher in M and SL groups than in group C. Additionally, serum triglyceride levels, LDL-cholesterol and total cholesterol were also significantly higher in M and SL groups when compared to the control group. Serum phospholipids fatty acids varied in response to total dietary fat. A significant decrease in saturated fatty acids (SFA) content (16:0 and 18:0) and an increase in monounsaturated fatty acid (MUFA) content (18:1, n-9) was found in the M group when compared to both C and SL groups. In the SL group, SFA content (18:0) was higher and MUFA content (18:1, n-9) was lower than in group C. Polyunsaturated fatty acids (PUFA) content showed an increase in both experimental groups. The PUFA/SFA ratio was higher in the M group than in the C and SL groups. Our study suggests that the amount of dietary fat has a greater influence on obesity than the effects of the type of fat consumed. However, depending on the type of fat present in the diet the differences were observed in the composition of serum PL fatty acid suggesting that both total fat and individual fatty acids have to be considered when reaching conclusions about the effect of dietary fat and obesity in animals. AB - Uticaj masnih kiselina u ishrani i njihov efekat na pojavu gojaznosti kod životinja je kontraverzno pitanje već dugi niz godina. Uticaj pojedinačnih masnih kiselina na pojavu gojaznosti kod životinja nije do sada detaljnije proučavan. Sa ciljem da se ispita efekat dijeta sa niskim ili visokim sadržajem masti na pojavu gojaznosti, izvršena su istraživanja na Wister pacovima u trajanju od 4 nedelje. Pacovi su hranjeni eksperimentalnim dijetama koje su sadržale standardan, nizak (10%) ili visok (46%) energetski unos poreklom od masti. Grupa kontrolnih životinja (C) je hranjena standardnom laboratorijskom dijetom (odnos polizasićenih/ zasićenih masnih kiselina/PUFA, P/S 1.3), grupa M sa standardnom dijetom sa dodatkom margarina (P/S 0.95) i grupa SL sa dijetom kojoj je dodata mešavina 1:1 suncokretovog ulja i masti (odnos P/S 1.3). Obe dijete (M i SL) su uzrokovale povećanje u prosečnom dnevnom energetskom unosu. U grupi M i SL, registrovano je značajno povećanje u telesnoj težini kontinuirano tokom eksperimenta. Nivo serumskih triglicerida, LDL kao i ukupnog holesterola su bili značajnije povećani u M i SL grupi u poređenju sa kontrolom. Promene u profilu serumskih masnih kiselina fosfolipida su zavisile od sastava masti u eksperimentalnoj dijeti. Kod eksperimentalnih životinja grupe M primećeno je značajno smanjene serumskih zasićenih masnih kiselina (SFA, 16:0 i 18:0) kao i porast monozasićenih masnih kiselina (MUFA, 18:1, n-9) u poređenju sa SL i C grupom. Odnos PUFA/ SFA je bio veći u M u odnosu na SL i C grupu. Rezultati naših istraživanja ukazuju da količina masti u ishrani životinja ima veći uticaj na porast telesne mase nego vrsta prisutne masnoće. Promene u sastavu serumskih masnih kiselina fosfolipida u odnosu na tip masti u eksperimentalnim dijetama ukazuju na ulogu pojedinačnih masnih kiselina u procesu nastajanja gojaznosti kod životinja i te mehanizme treba dalje istraživati. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - Influence of dietary fats on serum phospholipid fatty acid composition and its relation to obesity in animals T1 - Uticaj masti u obroku na masne kiseline u serumskim fosfolipidima i gojaznost životinja EP - 41 IS - 1 SP - 33 VL - 58 DO - 10.2298/AVB0801033T ER -
@article{ author = "Tepšić, Vesna and Pavlović, Mirjana A. and Ristić-Medić, Danijela and Ristić, Vanja and Lekić, N. and Tepšić, Jasna and Debeljak-Martačić, Jasmina and Milićević, Miroslav and Glibetić, Marija", year = "2008", abstract = "Dietary fat and its relation to obesity has been a controversial issue for many years. Experimental data shows that most, though not all animals, which consume a high fat diet, will become obese. However, the effect of fatty acids on animal obesity has not been studied in detail. In order to evaluate the effects of low versus high fat diet on serum phospholipids fatty acids composition a 4-wk study was conducted on male Wister rats. The rats were fed low-fat (10% energy) and high-fat (46% energy) foods containing constant proportions of fatty acids. Control group C was fed a standard laboratory diet (polyunsaturated/ saturated (P/S) fatty ratio 1.3), group M was fed a standard laboratory diet supplemented with margarine (P/S ratio 0.95), and the diet of the SL group was additionally supplemented with a sunflower oil-lard (1:1) mixture (P/S ratio 1.3). All lipid supplemented hyperenergetic diets caused an increase in the average daily energy intake. Both the final and the daily body weight gain were significantly higher in M and SL groups than in group C. Additionally, serum triglyceride levels, LDL-cholesterol and total cholesterol were also significantly higher in M and SL groups when compared to the control group. Serum phospholipids fatty acids varied in response to total dietary fat. A significant decrease in saturated fatty acids (SFA) content (16:0 and 18:0) and an increase in monounsaturated fatty acid (MUFA) content (18:1, n-9) was found in the M group when compared to both C and SL groups. In the SL group, SFA content (18:0) was higher and MUFA content (18:1, n-9) was lower than in group C. Polyunsaturated fatty acids (PUFA) content showed an increase in both experimental groups. The PUFA/SFA ratio was higher in the M group than in the C and SL groups. Our study suggests that the amount of dietary fat has a greater influence on obesity than the effects of the type of fat consumed. However, depending on the type of fat present in the diet the differences were observed in the composition of serum PL fatty acid suggesting that both total fat and individual fatty acids have to be considered when reaching conclusions about the effect of dietary fat and obesity in animals., Uticaj masnih kiselina u ishrani i njihov efekat na pojavu gojaznosti kod životinja je kontraverzno pitanje već dugi niz godina. Uticaj pojedinačnih masnih kiselina na pojavu gojaznosti kod životinja nije do sada detaljnije proučavan. Sa ciljem da se ispita efekat dijeta sa niskim ili visokim sadržajem masti na pojavu gojaznosti, izvršena su istraživanja na Wister pacovima u trajanju od 4 nedelje. Pacovi su hranjeni eksperimentalnim dijetama koje su sadržale standardan, nizak (10%) ili visok (46%) energetski unos poreklom od masti. Grupa kontrolnih životinja (C) je hranjena standardnom laboratorijskom dijetom (odnos polizasićenih/ zasićenih masnih kiselina/PUFA, P/S 1.3), grupa M sa standardnom dijetom sa dodatkom margarina (P/S 0.95) i grupa SL sa dijetom kojoj je dodata mešavina 1:1 suncokretovog ulja i masti (odnos P/S 1.3). Obe dijete (M i SL) su uzrokovale povećanje u prosečnom dnevnom energetskom unosu. U grupi M i SL, registrovano je značajno povećanje u telesnoj težini kontinuirano tokom eksperimenta. Nivo serumskih triglicerida, LDL kao i ukupnog holesterola su bili značajnije povećani u M i SL grupi u poređenju sa kontrolom. Promene u profilu serumskih masnih kiselina fosfolipida su zavisile od sastava masti u eksperimentalnoj dijeti. Kod eksperimentalnih životinja grupe M primećeno je značajno smanjene serumskih zasićenih masnih kiselina (SFA, 16:0 i 18:0) kao i porast monozasićenih masnih kiselina (MUFA, 18:1, n-9) u poređenju sa SL i C grupom. Odnos PUFA/ SFA je bio veći u M u odnosu na SL i C grupu. Rezultati naših istraživanja ukazuju da količina masti u ishrani životinja ima veći uticaj na porast telesne mase nego vrsta prisutne masnoće. Promene u sastavu serumskih masnih kiselina fosfolipida u odnosu na tip masti u eksperimentalnim dijetama ukazuju na ulogu pojedinačnih masnih kiselina u procesu nastajanja gojaznosti kod životinja i te mehanizme treba dalje istraživati.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "Influence of dietary fats on serum phospholipid fatty acid composition and its relation to obesity in animals, Uticaj masti u obroku na masne kiseline u serumskim fosfolipidima i gojaznost životinja", pages = "41-33", number = "1", volume = "58", doi = "10.2298/AVB0801033T" }
Tepšić, V., Pavlović, M. A., Ristić-Medić, D., Ristić, V., Lekić, N., Tepšić, J., Debeljak-Martačić, J., Milićević, M.,& Glibetić, M.. (2008). Influence of dietary fats on serum phospholipid fatty acid composition and its relation to obesity in animals. in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 58(1), 33-41. https://doi.org/10.2298/AVB0801033T
Tepšić V, Pavlović MA, Ristić-Medić D, Ristić V, Lekić N, Tepšić J, Debeljak-Martačić J, Milićević M, Glibetić M. Influence of dietary fats on serum phospholipid fatty acid composition and its relation to obesity in animals. in Acta Veterinaria-Beograd. 2008;58(1):33-41. doi:10.2298/AVB0801033T .
Tepšić, Vesna, Pavlović, Mirjana A., Ristić-Medić, Danijela, Ristić, Vanja, Lekić, N., Tepšić, Jasna, Debeljak-Martačić, Jasmina, Milićević, Miroslav, Glibetić, Marija, "Influence of dietary fats on serum phospholipid fatty acid composition and its relation to obesity in animals" in Acta Veterinaria-Beograd, 58, no. 1 (2008):33-41, https://doi.org/10.2298/AVB0801033T . .