Signaling pathways implicated in hematopoietic progenitor cell proliferation and differentiation
Nema prikaza
Autori
Bugarski, DianaKrstić, Aleksandra
Mojsilović, Slavko
Vlaški, Marija
Petakov, Marijana
Jovčić, Gordana
Stojanović, Nevenka
Milenković, Pavle B.
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The objective of this study was to investigate the signal transduction pathways associated with the clonal development of myeloid and erythroid progenitor cells. The contribution of particular signaling molecules of protein tyrosine kinases (PTKs), mitogen-activated protein (MAP) kinase, and PI-3 kinase signaling to the growth of murine bone marrow colony forming unit-granulocyte-macrophage (CFU-GM) and erythroid (burst forming unit-erythroid [BFU-E] and colony forming unit-erythroid [CFU-E]) progenitors was examined in studies performed in the presence or absence of specific signal transduction inhibitors. The results clearly pointed to different signal transducing intermediates that are involved in cell proliferation and differentiation depending on the cell lineage, as well as on the progenitors' maturity. Lineage-specific differences were obtained when chemical inhibitors specific for receptor- or nonreceptor-PTKs, as well as for the main groups of distinctly regulated MAPK cascade...s, were used because all of these compounds suppressed the growth of erythroid progenitors, with no major effects on myeloid progenitors. At the same time, differential involvement of MEK/extracellular signal-regulated kinase (ERK) MAPK transduction pathway was observed in the proliferation and/or differentiation of early, BFU-E, and late, CFU-E, erythroid progenitor cells. The results also demonstrated that phosphatydylinositol (PI)-3 kinase and nuclear factor kappaB (NF-kappa B) transcriptional factor were required for maintenance of both myeloid and erythroid progenitor cell function. Overall, the data obtained indicated that committed hematopoietic progenitors express a certain level of constitutive signaling activity that participates in the regulation of normal steady-state hematopoiesis and point to the importance of evaluating the impact of signal transduction inhibitors on normal bone marrow when used as potential therapeutic agents.
Ključne reči:
signal transduction / hematopoiesis / bone marrow cells / hematopoietic progenitorsIzvor:
Experimental Biology & Medicine, 2007, 232, 1, 156-163Izdavač:
- Sage Publications Ltd, London
Institucija/grupa
Institut za medicinska istraživanjaTY - JOUR AU - Bugarski, Diana AU - Krstić, Aleksandra AU - Mojsilović, Slavko AU - Vlaški, Marija AU - Petakov, Marijana AU - Jovčić, Gordana AU - Stojanović, Nevenka AU - Milenković, Pavle B. PY - 2007 UR - http://rimi.imi.bg.ac.rs/handle/123456789/178 AB - The objective of this study was to investigate the signal transduction pathways associated with the clonal development of myeloid and erythroid progenitor cells. The contribution of particular signaling molecules of protein tyrosine kinases (PTKs), mitogen-activated protein (MAP) kinase, and PI-3 kinase signaling to the growth of murine bone marrow colony forming unit-granulocyte-macrophage (CFU-GM) and erythroid (burst forming unit-erythroid [BFU-E] and colony forming unit-erythroid [CFU-E]) progenitors was examined in studies performed in the presence or absence of specific signal transduction inhibitors. The results clearly pointed to different signal transducing intermediates that are involved in cell proliferation and differentiation depending on the cell lineage, as well as on the progenitors' maturity. Lineage-specific differences were obtained when chemical inhibitors specific for receptor- or nonreceptor-PTKs, as well as for the main groups of distinctly regulated MAPK cascades, were used because all of these compounds suppressed the growth of erythroid progenitors, with no major effects on myeloid progenitors. At the same time, differential involvement of MEK/extracellular signal-regulated kinase (ERK) MAPK transduction pathway was observed in the proliferation and/or differentiation of early, BFU-E, and late, CFU-E, erythroid progenitor cells. The results also demonstrated that phosphatydylinositol (PI)-3 kinase and nuclear factor kappaB (NF-kappa B) transcriptional factor were required for maintenance of both myeloid and erythroid progenitor cell function. Overall, the data obtained indicated that committed hematopoietic progenitors express a certain level of constitutive signaling activity that participates in the regulation of normal steady-state hematopoiesis and point to the importance of evaluating the impact of signal transduction inhibitors on normal bone marrow when used as potential therapeutic agents. PB - Sage Publications Ltd, London T2 - Experimental Biology & Medicine T1 - Signaling pathways implicated in hematopoietic progenitor cell proliferation and differentiation EP - 163 IS - 1 SP - 156 VL - 232 UR - https://hdl.handle.net/21.15107/rcub_rimi_178 ER -
@article{ author = "Bugarski, Diana and Krstić, Aleksandra and Mojsilović, Slavko and Vlaški, Marija and Petakov, Marijana and Jovčić, Gordana and Stojanović, Nevenka and Milenković, Pavle B.", year = "2007", abstract = "The objective of this study was to investigate the signal transduction pathways associated with the clonal development of myeloid and erythroid progenitor cells. The contribution of particular signaling molecules of protein tyrosine kinases (PTKs), mitogen-activated protein (MAP) kinase, and PI-3 kinase signaling to the growth of murine bone marrow colony forming unit-granulocyte-macrophage (CFU-GM) and erythroid (burst forming unit-erythroid [BFU-E] and colony forming unit-erythroid [CFU-E]) progenitors was examined in studies performed in the presence or absence of specific signal transduction inhibitors. The results clearly pointed to different signal transducing intermediates that are involved in cell proliferation and differentiation depending on the cell lineage, as well as on the progenitors' maturity. Lineage-specific differences were obtained when chemical inhibitors specific for receptor- or nonreceptor-PTKs, as well as for the main groups of distinctly regulated MAPK cascades, were used because all of these compounds suppressed the growth of erythroid progenitors, with no major effects on myeloid progenitors. At the same time, differential involvement of MEK/extracellular signal-regulated kinase (ERK) MAPK transduction pathway was observed in the proliferation and/or differentiation of early, BFU-E, and late, CFU-E, erythroid progenitor cells. The results also demonstrated that phosphatydylinositol (PI)-3 kinase and nuclear factor kappaB (NF-kappa B) transcriptional factor were required for maintenance of both myeloid and erythroid progenitor cell function. Overall, the data obtained indicated that committed hematopoietic progenitors express a certain level of constitutive signaling activity that participates in the regulation of normal steady-state hematopoiesis and point to the importance of evaluating the impact of signal transduction inhibitors on normal bone marrow when used as potential therapeutic agents.", publisher = "Sage Publications Ltd, London", journal = "Experimental Biology & Medicine", title = "Signaling pathways implicated in hematopoietic progenitor cell proliferation and differentiation", pages = "163-156", number = "1", volume = "232", url = "https://hdl.handle.net/21.15107/rcub_rimi_178" }
Bugarski, D., Krstić, A., Mojsilović, S., Vlaški, M., Petakov, M., Jovčić, G., Stojanović, N.,& Milenković, P. B.. (2007). Signaling pathways implicated in hematopoietic progenitor cell proliferation and differentiation. in Experimental Biology & Medicine Sage Publications Ltd, London., 232(1), 156-163. https://hdl.handle.net/21.15107/rcub_rimi_178
Bugarski D, Krstić A, Mojsilović S, Vlaški M, Petakov M, Jovčić G, Stojanović N, Milenković PB. Signaling pathways implicated in hematopoietic progenitor cell proliferation and differentiation. in Experimental Biology & Medicine. 2007;232(1):156-163. https://hdl.handle.net/21.15107/rcub_rimi_178 .
Bugarski, Diana, Krstić, Aleksandra, Mojsilović, Slavko, Vlaški, Marija, Petakov, Marijana, Jovčić, Gordana, Stojanović, Nevenka, Milenković, Pavle B., "Signaling pathways implicated in hematopoietic progenitor cell proliferation and differentiation" in Experimental Biology & Medicine, 232, no. 1 (2007):156-163, https://hdl.handle.net/21.15107/rcub_rimi_178 .