Interleukin-6 (IL-6) and low O-2 concentration (1%) synergize to improve the maintenance of hematopoietic stem cells (Pre-CFC)
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2007
Authors
Kovačević-Filipović, Milica
Petakov, Marijana
Hermitte, Francis
Debeissat, Christelle

Krstić, Aleksandra

Jovčić, Gordana
Biligarski, Dijana
Lafarge, Xavier

Milenković, Pavle B.

Praloran, Vincent
Ivanović, Zoran

Article (Published version)

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Low O-2 concentration (1%) favors the self-renewal of hematopoietic stem cells and inhibits committed progenitors (CFC). Since IL-6 influences both stem cells and committed progenitors at 20% O-2, we studied its effects in cultures at 1% O-2. The pre-CFC activity in Lin- population of mouse bone marrow was analyzed following 10 days of serum-free culture in medium (LCI) supplemented with IL-3 with and without IL-6, at 20 and 1% O-2 and phenotypic differentiation and proliferative history monitored. The IL-6 receptor expression and initiation of VEGF-A synthesis were also investigated. At 20% O-2, the effects of IL-6 on pre-CFC were negligible but effects on CFC were apparent; conversely, at 1% O-2, the IL-6 enhances activity of pre-CFC but not of CFC. Unlike at 20% O-2, at 1% O-2 a subpopulation of cells remained Lin- in spite of extensive proliferation. However, the absolute number of Lin- cells, did not correlate with pre-CFC activity. A relative increase in VEGF transcripts at 1% O-...2 in presence of IL-3 alone was enhanced by the addition of IL-6. IL-6 enhanced pre-CFC activity at 1% O-2 and this was correlated to the induction of VEGF. These data reinforce the concept that physiologically low oxygenation of bone marrow is a regulator of stem cell maintenance. Since the 20% O-2 does not exist in tissues in vivo, further studies in vitro at lower O-2 concentrations should revise our knowledge relating to cytokine effects on stem and progenitor cells.
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Journal of Cellular Physiology, 2007, 212, 1, 68-75Publisher:
- Wiley, Hoboken
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DOI: 10.1002/jcp.21003
ISSN: 0021-9541
PubMed: 17311290
WoS: 000246789300008
Scopus: 2-s2.0-34249905641
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Institut za medicinska istraživanjaTY - JOUR AU - Kovačević-Filipović, Milica AU - Petakov, Marijana AU - Hermitte, Francis AU - Debeissat, Christelle AU - Krstić, Aleksandra AU - Jovčić, Gordana AU - Biligarski, Dijana AU - Lafarge, Xavier AU - Milenković, Pavle B. AU - Praloran, Vincent AU - Ivanović, Zoran PY - 2007 UR - http://rimi.imi.bg.ac.rs/handle/123456789/171 AB - Low O-2 concentration (1%) favors the self-renewal of hematopoietic stem cells and inhibits committed progenitors (CFC). Since IL-6 influences both stem cells and committed progenitors at 20% O-2, we studied its effects in cultures at 1% O-2. The pre-CFC activity in Lin- population of mouse bone marrow was analyzed following 10 days of serum-free culture in medium (LCI) supplemented with IL-3 with and without IL-6, at 20 and 1% O-2 and phenotypic differentiation and proliferative history monitored. The IL-6 receptor expression and initiation of VEGF-A synthesis were also investigated. At 20% O-2, the effects of IL-6 on pre-CFC were negligible but effects on CFC were apparent; conversely, at 1% O-2, the IL-6 enhances activity of pre-CFC but not of CFC. Unlike at 20% O-2, at 1% O-2 a subpopulation of cells remained Lin- in spite of extensive proliferation. However, the absolute number of Lin- cells, did not correlate with pre-CFC activity. A relative increase in VEGF transcripts at 1% O-2 in presence of IL-3 alone was enhanced by the addition of IL-6. IL-6 enhanced pre-CFC activity at 1% O-2 and this was correlated to the induction of VEGF. These data reinforce the concept that physiologically low oxygenation of bone marrow is a regulator of stem cell maintenance. Since the 20% O-2 does not exist in tissues in vivo, further studies in vitro at lower O-2 concentrations should revise our knowledge relating to cytokine effects on stem and progenitor cells. PB - Wiley, Hoboken T2 - Journal of Cellular Physiology T1 - Interleukin-6 (IL-6) and low O-2 concentration (1%) synergize to improve the maintenance of hematopoietic stem cells (Pre-CFC) EP - 75 IS - 1 SP - 68 VL - 212 DO - 10.1002/jcp.21003 UR - conv_1823 ER -
@article{ author = "Kovačević-Filipović, Milica and Petakov, Marijana and Hermitte, Francis and Debeissat, Christelle and Krstić, Aleksandra and Jovčić, Gordana and Biligarski, Dijana and Lafarge, Xavier and Milenković, Pavle B. and Praloran, Vincent and Ivanović, Zoran", year = "2007", abstract = "Low O-2 concentration (1%) favors the self-renewal of hematopoietic stem cells and inhibits committed progenitors (CFC). Since IL-6 influences both stem cells and committed progenitors at 20% O-2, we studied its effects in cultures at 1% O-2. The pre-CFC activity in Lin- population of mouse bone marrow was analyzed following 10 days of serum-free culture in medium (LCI) supplemented with IL-3 with and without IL-6, at 20 and 1% O-2 and phenotypic differentiation and proliferative history monitored. The IL-6 receptor expression and initiation of VEGF-A synthesis were also investigated. At 20% O-2, the effects of IL-6 on pre-CFC were negligible but effects on CFC were apparent; conversely, at 1% O-2, the IL-6 enhances activity of pre-CFC but not of CFC. Unlike at 20% O-2, at 1% O-2 a subpopulation of cells remained Lin- in spite of extensive proliferation. However, the absolute number of Lin- cells, did not correlate with pre-CFC activity. A relative increase in VEGF transcripts at 1% O-2 in presence of IL-3 alone was enhanced by the addition of IL-6. IL-6 enhanced pre-CFC activity at 1% O-2 and this was correlated to the induction of VEGF. These data reinforce the concept that physiologically low oxygenation of bone marrow is a regulator of stem cell maintenance. Since the 20% O-2 does not exist in tissues in vivo, further studies in vitro at lower O-2 concentrations should revise our knowledge relating to cytokine effects on stem and progenitor cells.", publisher = "Wiley, Hoboken", journal = "Journal of Cellular Physiology", title = "Interleukin-6 (IL-6) and low O-2 concentration (1%) synergize to improve the maintenance of hematopoietic stem cells (Pre-CFC)", pages = "75-68", number = "1", volume = "212", doi = "10.1002/jcp.21003", url = "conv_1823" }
Kovačević-Filipović, M., Petakov, M., Hermitte, F., Debeissat, C., Krstić, A., Jovčić, G., Biligarski, D., Lafarge, X., Milenković, P. B., Praloran, V.,& Ivanović, Z.. (2007). Interleukin-6 (IL-6) and low O-2 concentration (1%) synergize to improve the maintenance of hematopoietic stem cells (Pre-CFC). in Journal of Cellular Physiology Wiley, Hoboken., 212(1), 68-75. https://doi.org/10.1002/jcp.21003 conv_1823
Kovačević-Filipović M, Petakov M, Hermitte F, Debeissat C, Krstić A, Jovčić G, Biligarski D, Lafarge X, Milenković PB, Praloran V, Ivanović Z. Interleukin-6 (IL-6) and low O-2 concentration (1%) synergize to improve the maintenance of hematopoietic stem cells (Pre-CFC). in Journal of Cellular Physiology. 2007;212(1):68-75. doi:10.1002/jcp.21003 conv_1823 .
Kovačević-Filipović, Milica, Petakov, Marijana, Hermitte, Francis, Debeissat, Christelle, Krstić, Aleksandra, Jovčić, Gordana, Biligarski, Dijana, Lafarge, Xavier, Milenković, Pavle B., Praloran, Vincent, Ivanović, Zoran, "Interleukin-6 (IL-6) and low O-2 concentration (1%) synergize to improve the maintenance of hematopoietic stem cells (Pre-CFC)" in Journal of Cellular Physiology, 212, no. 1 (2007):68-75, https://doi.org/10.1002/jcp.21003 ., conv_1823 .