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Interleukin-6 (IL-6) and low O-2 concentration (1%) synergize to improve the maintenance of hematopoietic stem cells (Pre-CFC)

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2007
Authors
Kovačević-Filipović, Milica
Petakov, Marijana
Hermitte, Francis
Debeissat, Christelle
Krstić, Aleksandra
Jovčić, Gordana
Biligarski, Dijana
Lafarge, Xavier
Milenković, Pavle B.
Praloran, Vincent
Ivanović, Zoran
Article (Published version)
Metadata
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Abstract
Low O-2 concentration (1%) favors the self-renewal of hematopoietic stem cells and inhibits committed progenitors (CFC). Since IL-6 influences both stem cells and committed progenitors at 20% O-2, we studied its effects in cultures at 1% O-2. The pre-CFC activity in Lin- population of mouse bone marrow was analyzed following 10 days of serum-free culture in medium (LCI) supplemented with IL-3 with and without IL-6, at 20 and 1% O-2 and phenotypic differentiation and proliferative history monitored. The IL-6 receptor expression and initiation of VEGF-A synthesis were also investigated. At 20% O-2, the effects of IL-6 on pre-CFC were negligible but effects on CFC were apparent; conversely, at 1% O-2, the IL-6 enhances activity of pre-CFC but not of CFC. Unlike at 20% O-2, at 1% O-2 a subpopulation of cells remained Lin- in spite of extensive proliferation. However, the absolute number of Lin- cells, did not correlate with pre-CFC activity. A relative increase in VEGF transcripts at 1% O-...2 in presence of IL-3 alone was enhanced by the addition of IL-6. IL-6 enhanced pre-CFC activity at 1% O-2 and this was correlated to the induction of VEGF. These data reinforce the concept that physiologically low oxygenation of bone marrow is a regulator of stem cell maintenance. Since the 20% O-2 does not exist in tissues in vivo, further studies in vitro at lower O-2 concentrations should revise our knowledge relating to cytokine effects on stem and progenitor cells.

Source:
Journal of Cellular Physiology, 2007, 212, 1, 68-75
Publisher:
  • Wiley, Hoboken
Funding / projects:
  • Ćelijski i molekularni mehanizmi regilacije hematopoeze (RS-145048)

DOI: 10.1002/jcp.21003

ISSN: 0021-9541

PubMed: 17311290

WoS: 000246789300008

Scopus: 2-s2.0-34249905641
[ Google Scholar ]
31
30
URI
http://rimi.imi.bg.ac.rs/handle/123456789/171
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
Institut za medicinska istraživanja
TY  - JOUR
AU  - Kovačević-Filipović, Milica
AU  - Petakov, Marijana
AU  - Hermitte, Francis
AU  - Debeissat, Christelle
AU  - Krstić, Aleksandra
AU  - Jovčić, Gordana
AU  - Biligarski, Dijana
AU  - Lafarge, Xavier
AU  - Milenković, Pavle B.
AU  - Praloran, Vincent
AU  - Ivanović, Zoran
PY  - 2007
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/171
AB  - Low O-2 concentration (1%) favors the self-renewal of hematopoietic stem cells and inhibits committed progenitors (CFC). Since IL-6 influences both stem cells and committed progenitors at 20% O-2, we studied its effects in cultures at 1% O-2. The pre-CFC activity in Lin- population of mouse bone marrow was analyzed following 10 days of serum-free culture in medium (LCI) supplemented with IL-3 with and without IL-6, at 20 and 1% O-2 and phenotypic differentiation and proliferative history monitored. The IL-6 receptor expression and initiation of VEGF-A synthesis were also investigated. At 20% O-2, the effects of IL-6 on pre-CFC were negligible but effects on CFC were apparent; conversely, at 1% O-2, the IL-6 enhances activity of pre-CFC but not of CFC. Unlike at 20% O-2, at 1% O-2 a subpopulation of cells remained Lin- in spite of extensive proliferation. However, the absolute number of Lin- cells, did not correlate with pre-CFC activity. A relative increase in VEGF transcripts at 1% O-2 in presence of IL-3 alone was enhanced by the addition of IL-6. IL-6 enhanced pre-CFC activity at 1% O-2 and this was correlated to the induction of VEGF. These data reinforce the concept that physiologically low oxygenation of bone marrow is a regulator of stem cell maintenance. Since the 20% O-2 does not exist in tissues in vivo, further studies in vitro at lower O-2 concentrations should revise our knowledge relating to cytokine effects on stem and progenitor cells.
PB  - Wiley, Hoboken
T2  - Journal of Cellular Physiology
T1  - Interleukin-6 (IL-6) and low O-2 concentration (1%) synergize to improve the maintenance of hematopoietic stem cells (Pre-CFC)
EP  - 75
IS  - 1
SP  - 68
VL  - 212
DO  - 10.1002/jcp.21003
UR  - conv_1823
ER  - 
@article{
author = "Kovačević-Filipović, Milica and Petakov, Marijana and Hermitte, Francis and Debeissat, Christelle and Krstić, Aleksandra and Jovčić, Gordana and Biligarski, Dijana and Lafarge, Xavier and Milenković, Pavle B. and Praloran, Vincent and Ivanović, Zoran",
year = "2007",
abstract = "Low O-2 concentration (1%) favors the self-renewal of hematopoietic stem cells and inhibits committed progenitors (CFC). Since IL-6 influences both stem cells and committed progenitors at 20% O-2, we studied its effects in cultures at 1% O-2. The pre-CFC activity in Lin- population of mouse bone marrow was analyzed following 10 days of serum-free culture in medium (LCI) supplemented with IL-3 with and without IL-6, at 20 and 1% O-2 and phenotypic differentiation and proliferative history monitored. The IL-6 receptor expression and initiation of VEGF-A synthesis were also investigated. At 20% O-2, the effects of IL-6 on pre-CFC were negligible but effects on CFC were apparent; conversely, at 1% O-2, the IL-6 enhances activity of pre-CFC but not of CFC. Unlike at 20% O-2, at 1% O-2 a subpopulation of cells remained Lin- in spite of extensive proliferation. However, the absolute number of Lin- cells, did not correlate with pre-CFC activity. A relative increase in VEGF transcripts at 1% O-2 in presence of IL-3 alone was enhanced by the addition of IL-6. IL-6 enhanced pre-CFC activity at 1% O-2 and this was correlated to the induction of VEGF. These data reinforce the concept that physiologically low oxygenation of bone marrow is a regulator of stem cell maintenance. Since the 20% O-2 does not exist in tissues in vivo, further studies in vitro at lower O-2 concentrations should revise our knowledge relating to cytokine effects on stem and progenitor cells.",
publisher = "Wiley, Hoboken",
journal = "Journal of Cellular Physiology",
title = "Interleukin-6 (IL-6) and low O-2 concentration (1%) synergize to improve the maintenance of hematopoietic stem cells (Pre-CFC)",
pages = "75-68",
number = "1",
volume = "212",
doi = "10.1002/jcp.21003",
url = "conv_1823"
}
Kovačević-Filipović, M., Petakov, M., Hermitte, F., Debeissat, C., Krstić, A., Jovčić, G., Biligarski, D., Lafarge, X., Milenković, P. B., Praloran, V.,& Ivanović, Z.. (2007). Interleukin-6 (IL-6) and low O-2 concentration (1%) synergize to improve the maintenance of hematopoietic stem cells (Pre-CFC). in Journal of Cellular Physiology
Wiley, Hoboken., 212(1), 68-75.
https://doi.org/10.1002/jcp.21003
conv_1823
Kovačević-Filipović M, Petakov M, Hermitte F, Debeissat C, Krstić A, Jovčić G, Biligarski D, Lafarge X, Milenković PB, Praloran V, Ivanović Z. Interleukin-6 (IL-6) and low O-2 concentration (1%) synergize to improve the maintenance of hematopoietic stem cells (Pre-CFC). in Journal of Cellular Physiology. 2007;212(1):68-75.
doi:10.1002/jcp.21003
conv_1823 .
Kovačević-Filipović, Milica, Petakov, Marijana, Hermitte, Francis, Debeissat, Christelle, Krstić, Aleksandra, Jovčić, Gordana, Biligarski, Dijana, Lafarge, Xavier, Milenković, Pavle B., Praloran, Vincent, Ivanović, Zoran, "Interleukin-6 (IL-6) and low O-2 concentration (1%) synergize to improve the maintenance of hematopoietic stem cells (Pre-CFC)" in Journal of Cellular Physiology, 212, no. 1 (2007):68-75,
https://doi.org/10.1002/jcp.21003 .,
conv_1823 .

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