Possible mechanism of acute effect of ethanol on intestinal IgA expression in rat
Samo za registrovane korisnike
2007
Autori
Budeč, Mirela
Koko, Vesna
Todorović, Vera
Marković, Dragana

Poštić, Marija M.

Drndarević, Neda
Spasić, Anđelka
Mitrović, Olivera

Članak u časopisu (Objavljena verzija)

Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The purpose of this study was to investigate the possible mechanism of acute effect of ethanol on IgA expression in rat intestine. To this end, adult female Wistar rats showing diestrus day I were treated with (a) ethanol (2 or 4 g/kg, i.p.); (b) N omega-nitro-L-arginine-methyl ester (L-NAME), which inhibits the activity of all isoforms of nitric oxide synthase, (30 mg/kg, s.c.) followed by ethanol 3 h later-, and (C) L-NAME (30 mg/kg, s.c.) followed by saline 3 h later. Saline-injected and untreated rats were used as controls. The animals were sacrificed 0.5 h after ethanol administration. Intestinal expression of IgA was evaluated by both immunohistochemistry and Western immunoblotting. Morphometric analysis showed that acute ethanol treatment increased the number of IgA-immunoreactive cells in a dose-dependent manner. Pretreatment with L-NAME abolished this action of alcohol. Injection of L-NAME followed by saline had no influence on the number of IgA+cells. The results, obtained by... Western immunoblotting, paralleled our immunohistochemical findings. Taken together, these data suggest that acute effect of ethanol on intestinal IgA might be mediated by endogenous nitric oxide.
Ključne reči:
ethanol / L-NAME / IgA / immunohistochemistry / Western blot / ratIzvor:
International Immunopharmacology, 2007, 7, 6, 858-863Izdavač:
- Elsevier Science Bv, Amsterdam
DOI: 10.1016/j.intimp.2007.02.010
ISSN: 1567-5769
PubMed: 17466919
WoS: 000246818200015
Scopus: 2-s2.0-34247528980
Institucija/grupa
Institut za medicinska istraživanjaTY - JOUR AU - Budeč, Mirela AU - Koko, Vesna AU - Todorović, Vera AU - Marković, Dragana AU - Poštić, Marija M. AU - Drndarević, Neda AU - Spasić, Anđelka AU - Mitrović, Olivera PY - 2007 UR - http://rimi.imi.bg.ac.rs/handle/123456789/170 AB - The purpose of this study was to investigate the possible mechanism of acute effect of ethanol on IgA expression in rat intestine. To this end, adult female Wistar rats showing diestrus day I were treated with (a) ethanol (2 or 4 g/kg, i.p.); (b) N omega-nitro-L-arginine-methyl ester (L-NAME), which inhibits the activity of all isoforms of nitric oxide synthase, (30 mg/kg, s.c.) followed by ethanol 3 h later-, and (C) L-NAME (30 mg/kg, s.c.) followed by saline 3 h later. Saline-injected and untreated rats were used as controls. The animals were sacrificed 0.5 h after ethanol administration. Intestinal expression of IgA was evaluated by both immunohistochemistry and Western immunoblotting. Morphometric analysis showed that acute ethanol treatment increased the number of IgA-immunoreactive cells in a dose-dependent manner. Pretreatment with L-NAME abolished this action of alcohol. Injection of L-NAME followed by saline had no influence on the number of IgA+cells. The results, obtained by Western immunoblotting, paralleled our immunohistochemical findings. Taken together, these data suggest that acute effect of ethanol on intestinal IgA might be mediated by endogenous nitric oxide. PB - Elsevier Science Bv, Amsterdam T2 - International Immunopharmacology T1 - Possible mechanism of acute effect of ethanol on intestinal IgA expression in rat EP - 863 IS - 6 SP - 858 VL - 7 DO - 10.1016/j.intimp.2007.02.010 ER -
@article{ author = "Budeč, Mirela and Koko, Vesna and Todorović, Vera and Marković, Dragana and Poštić, Marija M. and Drndarević, Neda and Spasić, Anđelka and Mitrović, Olivera", year = "2007", abstract = "The purpose of this study was to investigate the possible mechanism of acute effect of ethanol on IgA expression in rat intestine. To this end, adult female Wistar rats showing diestrus day I were treated with (a) ethanol (2 or 4 g/kg, i.p.); (b) N omega-nitro-L-arginine-methyl ester (L-NAME), which inhibits the activity of all isoforms of nitric oxide synthase, (30 mg/kg, s.c.) followed by ethanol 3 h later-, and (C) L-NAME (30 mg/kg, s.c.) followed by saline 3 h later. Saline-injected and untreated rats were used as controls. The animals were sacrificed 0.5 h after ethanol administration. Intestinal expression of IgA was evaluated by both immunohistochemistry and Western immunoblotting. Morphometric analysis showed that acute ethanol treatment increased the number of IgA-immunoreactive cells in a dose-dependent manner. Pretreatment with L-NAME abolished this action of alcohol. Injection of L-NAME followed by saline had no influence on the number of IgA+cells. The results, obtained by Western immunoblotting, paralleled our immunohistochemical findings. Taken together, these data suggest that acute effect of ethanol on intestinal IgA might be mediated by endogenous nitric oxide.", publisher = "Elsevier Science Bv, Amsterdam", journal = "International Immunopharmacology", title = "Possible mechanism of acute effect of ethanol on intestinal IgA expression in rat", pages = "863-858", number = "6", volume = "7", doi = "10.1016/j.intimp.2007.02.010" }
Budeč, M., Koko, V., Todorović, V., Marković, D., Poštić, M. M., Drndarević, N., Spasić, A.,& Mitrović, O.. (2007). Possible mechanism of acute effect of ethanol on intestinal IgA expression in rat. in International Immunopharmacology Elsevier Science Bv, Amsterdam., 7(6), 858-863. https://doi.org/10.1016/j.intimp.2007.02.010
Budeč M, Koko V, Todorović V, Marković D, Poštić MM, Drndarević N, Spasić A, Mitrović O. Possible mechanism of acute effect of ethanol on intestinal IgA expression in rat. in International Immunopharmacology. 2007;7(6):858-863. doi:10.1016/j.intimp.2007.02.010 .
Budeč, Mirela, Koko, Vesna, Todorović, Vera, Marković, Dragana, Poštić, Marija M., Drndarević, Neda, Spasić, Anđelka, Mitrović, Olivera, "Possible mechanism of acute effect of ethanol on intestinal IgA expression in rat" in International Immunopharmacology, 7, no. 6 (2007):858-863, https://doi.org/10.1016/j.intimp.2007.02.010 . .