The purpose of this study was to investigate the possible mechanism of acute effect of ethanol on IgA expression in rat intestine. To this end, adult female Wistar rats showing diestrus day I were treated with (a) ethanol (2 or 4 g/kg, i.p.); (b) N omega-nitro-L-arginine-methyl ester (L-NAME), which inhibits the activity of all isoforms of nitric oxide synthase, (30 mg/kg, s.c.) followed by ethanol 3 h later-, and (C) L-NAME (30 mg/kg, s.c.) followed by saline 3 h later. Saline-injected and untreated rats were used as controls. The animals were sacrificed 0.5 h after ethanol administration. Intestinal expression of IgA was evaluated by both immunohistochemistry and Western immunoblotting. Morphometric analysis showed that acute ethanol treatment increased the number of IgA-immunoreactive cells in a dose-dependent manner. Pretreatment with L-NAME abolished this action of alcohol. Injection of L-NAME followed by saline had no influence on the number of IgA+cells. The results, obtained by... Western immunoblotting, paralleled our immunohistochemical findings. Taken together, these data suggest that acute effect of ethanol on intestinal IgA might be mediated by endogenous nitric oxide.
Ključne reči:
ethanol / L-NAME / IgA / immunohistochemistry / Western blot / rat
Izvor:
International Immunopharmacology, 2007, 7, 6, 858-863
TY - JOUR
AU - Budeč, Mirela
AU - Koko, Vesna
AU - Todorović, Vera
AU - Marković, Dragana
AU - Poštić, Marija M.
AU - Drndarević, Neda
AU - Spasić, Anđelka
AU - Mitrović, Olivera
PY - 2007
UR - http://rimi.imi.bg.ac.rs/handle/123456789/170
AB - The purpose of this study was to investigate the possible mechanism of acute effect of ethanol on IgA expression in rat intestine. To this end, adult female Wistar rats showing diestrus day I were treated with (a) ethanol (2 or 4 g/kg, i.p.); (b) N omega-nitro-L-arginine-methyl ester (L-NAME), which inhibits the activity of all isoforms of nitric oxide synthase, (30 mg/kg, s.c.) followed by ethanol 3 h later-, and (C) L-NAME (30 mg/kg, s.c.) followed by saline 3 h later. Saline-injected and untreated rats were used as controls. The animals were sacrificed 0.5 h after ethanol administration. Intestinal expression of IgA was evaluated by both immunohistochemistry and Western immunoblotting. Morphometric analysis showed that acute ethanol treatment increased the number of IgA-immunoreactive cells in a dose-dependent manner. Pretreatment with L-NAME abolished this action of alcohol. Injection of L-NAME followed by saline had no influence on the number of IgA+cells. The results, obtained by Western immunoblotting, paralleled our immunohistochemical findings. Taken together, these data suggest that acute effect of ethanol on intestinal IgA might be mediated by endogenous nitric oxide.
PB - Elsevier Science Bv, Amsterdam
T2 - International Immunopharmacology
T1 - Possible mechanism of acute effect of ethanol on intestinal IgA expression in rat
EP - 863
IS - 6
SP - 858
VL - 7
DO - 10.1016/j.intimp.2007.02.010
ER -
@article{
author = "Budeč, Mirela and Koko, Vesna and Todorović, Vera and Marković, Dragana and Poštić, Marija M. and Drndarević, Neda and Spasić, Anđelka and Mitrović, Olivera",
year = "2007",
abstract = "The purpose of this study was to investigate the possible mechanism of acute effect of ethanol on IgA expression in rat intestine. To this end, adult female Wistar rats showing diestrus day I were treated with (a) ethanol (2 or 4 g/kg, i.p.); (b) N omega-nitro-L-arginine-methyl ester (L-NAME), which inhibits the activity of all isoforms of nitric oxide synthase, (30 mg/kg, s.c.) followed by ethanol 3 h later-, and (C) L-NAME (30 mg/kg, s.c.) followed by saline 3 h later. Saline-injected and untreated rats were used as controls. The animals were sacrificed 0.5 h after ethanol administration. Intestinal expression of IgA was evaluated by both immunohistochemistry and Western immunoblotting. Morphometric analysis showed that acute ethanol treatment increased the number of IgA-immunoreactive cells in a dose-dependent manner. Pretreatment with L-NAME abolished this action of alcohol. Injection of L-NAME followed by saline had no influence on the number of IgA+cells. The results, obtained by Western immunoblotting, paralleled our immunohistochemical findings. Taken together, these data suggest that acute effect of ethanol on intestinal IgA might be mediated by endogenous nitric oxide.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "International Immunopharmacology",
title = "Possible mechanism of acute effect of ethanol on intestinal IgA expression in rat",
pages = "863-858",
number = "6",
volume = "7",
doi = "10.1016/j.intimp.2007.02.010"
}
Budeč, M., Koko, V., Todorović, V., Marković, D., Poštić, M. M., Drndarević, N., Spasić, A.,& Mitrović, O.. (2007). Possible mechanism of acute effect of ethanol on intestinal IgA expression in rat. in International Immunopharmacology
Elsevier Science Bv, Amsterdam., 7(6), 858-863.
https://doi.org/10.1016/j.intimp.2007.02.010
Budeč M, Koko V, Todorović V, Marković D, Poštić MM, Drndarević N, Spasić A, Mitrović O. Possible mechanism of acute effect of ethanol on intestinal IgA expression in rat. in International Immunopharmacology. 2007;7(6):858-863.
doi:10.1016/j.intimp.2007.02.010 .
Budeč, Mirela, Koko, Vesna, Todorović, Vera, Marković, Dragana, Poštić, Marija M., Drndarević, Neda, Spasić, Anđelka, Mitrović, Olivera, "Possible mechanism of acute effect of ethanol on intestinal IgA expression in rat" in International Immunopharmacology, 7, no. 6 (2007):858-863,
https://doi.org/10.1016/j.intimp.2007.02.010 . .
