Alginate microbeads as a cell support for cartilage tissue engineering: Bioreactor studies
Само за регистроване кориснике
2007
Конференцијски прилог (Објављена верзија)

Метаподаци
Приказ свих података о документуАпстракт
Alginate was shown to be a suitable support for entrapment and cultivation of chondrocytes and bone marrow stromal cells, which under appropriate in vitro conditions synthesized cartilaginous components. The main limitation in these cultures may be low rates of mass transport through the alginate matrix governed by diffusion. In this study, we have designed and utilized a bioreactor system based on a packed bed of alginate beads with immobilized chondrogenic cells. Continuous medium perfusion provided convective mass transport through the packed bed, while small diameters of beads (2.5 mm and down to 500 mu m) ensured short diffusion distances to the immobilized cells. During up to 5 weeks of cultivation, the cells synthesized extracellular matrix components merging beads together and indicating potentials of this system for precise regulation of the cellular microenvironment in cartilage tissue engineering.
Кључне речи:
alginate / bioreactor / cartilage tissue engineering / cell immobilizationИзвор:
Research Trends in Contemporary Materials Science, 2007, 555, 417-+Издавач:
- Trans Tech Publications Ltd, Durnten-Zurich
Финансирање / пројекти:
- Интеракција имобилисаних ћелија, ткива и биолошки активних молекула у биореакторским системима (RS-142075)
Институција/група
Institut za medicinska istraživanjaTY - CONF AU - Obradović, Bojana AU - Osmokrović, A. AU - Bugarski, Branko AU - Bugarski, Diana AU - Vunjak-Novaković, Gordana PY - 2007 UR - http://rimi.imi.bg.ac.rs/handle/123456789/163 AB - Alginate was shown to be a suitable support for entrapment and cultivation of chondrocytes and bone marrow stromal cells, which under appropriate in vitro conditions synthesized cartilaginous components. The main limitation in these cultures may be low rates of mass transport through the alginate matrix governed by diffusion. In this study, we have designed and utilized a bioreactor system based on a packed bed of alginate beads with immobilized chondrogenic cells. Continuous medium perfusion provided convective mass transport through the packed bed, while small diameters of beads (2.5 mm and down to 500 mu m) ensured short diffusion distances to the immobilized cells. During up to 5 weeks of cultivation, the cells synthesized extracellular matrix components merging beads together and indicating potentials of this system for precise regulation of the cellular microenvironment in cartilage tissue engineering. PB - Trans Tech Publications Ltd, Durnten-Zurich C3 - Research Trends in Contemporary Materials Science T1 - Alginate microbeads as a cell support for cartilage tissue engineering: Bioreactor studies EP - + SP - 417 VL - 555 DO - 10.4028/www.scientific.net/MSF.555.417 ER -
@conference{ author = "Obradović, Bojana and Osmokrović, A. and Bugarski, Branko and Bugarski, Diana and Vunjak-Novaković, Gordana", year = "2007", abstract = "Alginate was shown to be a suitable support for entrapment and cultivation of chondrocytes and bone marrow stromal cells, which under appropriate in vitro conditions synthesized cartilaginous components. The main limitation in these cultures may be low rates of mass transport through the alginate matrix governed by diffusion. In this study, we have designed and utilized a bioreactor system based on a packed bed of alginate beads with immobilized chondrogenic cells. Continuous medium perfusion provided convective mass transport through the packed bed, while small diameters of beads (2.5 mm and down to 500 mu m) ensured short diffusion distances to the immobilized cells. During up to 5 weeks of cultivation, the cells synthesized extracellular matrix components merging beads together and indicating potentials of this system for precise regulation of the cellular microenvironment in cartilage tissue engineering.", publisher = "Trans Tech Publications Ltd, Durnten-Zurich", journal = "Research Trends in Contemporary Materials Science", title = "Alginate microbeads as a cell support for cartilage tissue engineering: Bioreactor studies", pages = "+-417", volume = "555", doi = "10.4028/www.scientific.net/MSF.555.417" }
Obradović, B., Osmokrović, A., Bugarski, B., Bugarski, D.,& Vunjak-Novaković, G.. (2007). Alginate microbeads as a cell support for cartilage tissue engineering: Bioreactor studies. in Research Trends in Contemporary Materials Science Trans Tech Publications Ltd, Durnten-Zurich., 555, 417-+. https://doi.org/10.4028/www.scientific.net/MSF.555.417
Obradović B, Osmokrović A, Bugarski B, Bugarski D, Vunjak-Novaković G. Alginate microbeads as a cell support for cartilage tissue engineering: Bioreactor studies. in Research Trends in Contemporary Materials Science. 2007;555:417-+. doi:10.4028/www.scientific.net/MSF.555.417 .
Obradović, Bojana, Osmokrović, A., Bugarski, Branko, Bugarski, Diana, Vunjak-Novaković, Gordana, "Alginate microbeads as a cell support for cartilage tissue engineering: Bioreactor studies" in Research Trends in Contemporary Materials Science, 555 (2007):417-+, https://doi.org/10.4028/www.scientific.net/MSF.555.417 . .