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Alginate microbeads as a cell support for cartilage tissue engineering: Bioreactor studies

Authorized Users Only
2007
Authors
Obradović, Bojana
Osmokrović, A.
Bugarski, Branko
Bugarski, Diana
Vunjak-Novaković, Gordana
Conference object (Published version)
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Abstract
Alginate was shown to be a suitable support for entrapment and cultivation of chondrocytes and bone marrow stromal cells, which under appropriate in vitro conditions synthesized cartilaginous components. The main limitation in these cultures may be low rates of mass transport through the alginate matrix governed by diffusion. In this study, we have designed and utilized a bioreactor system based on a packed bed of alginate beads with immobilized chondrogenic cells. Continuous medium perfusion provided convective mass transport through the packed bed, while small diameters of beads (2.5 mm and down to 500 mu m) ensured short diffusion distances to the immobilized cells. During up to 5 weeks of cultivation, the cells synthesized extracellular matrix components merging beads together and indicating potentials of this system for precise regulation of the cellular microenvironment in cartilage tissue engineering.
Keywords:
alginate / bioreactor / cartilage tissue engineering / cell immobilization
Source:
Research Trends in Contemporary Materials Science, 2007, 555, 417-+
Publisher:
  • Trans Tech Publications Ltd, Durnten-Zurich
Funding / projects:
  • Interakcija imobilisanih ćelija, tkiva i biološki aktivnih molekula u bioreaktorskim sistemima (RS-142075)

DOI: 10.4028/www.scientific.net/MSF.555.417

ISSN: 0255-5476

WoS: 000249653700067

[ Google Scholar ]
7
URI
http://rimi.imi.bg.ac.rs/handle/123456789/163
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
Institut za medicinska istraživanja
TY  - CONF
AU  - Obradović, Bojana
AU  - Osmokrović, A.
AU  - Bugarski, Branko
AU  - Bugarski, Diana
AU  - Vunjak-Novaković, Gordana
PY  - 2007
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/163
AB  - Alginate was shown to be a suitable support for entrapment and cultivation of chondrocytes and bone marrow stromal cells, which under appropriate in vitro conditions synthesized cartilaginous components. The main limitation in these cultures may be low rates of mass transport through the alginate matrix governed by diffusion. In this study, we have designed and utilized a bioreactor system based on a packed bed of alginate beads with immobilized chondrogenic cells. Continuous medium perfusion provided convective mass transport through the packed bed, while small diameters of beads (2.5 mm and down to 500 mu m) ensured short diffusion distances to the immobilized cells. During up to 5 weeks of cultivation, the cells synthesized extracellular matrix components merging beads together and indicating potentials of this system for precise regulation of the cellular microenvironment in cartilage tissue engineering.
PB  - Trans Tech Publications Ltd, Durnten-Zurich
C3  - Research Trends in Contemporary Materials Science
T1  - Alginate microbeads as a cell support for cartilage tissue engineering: Bioreactor studies
EP  - +
SP  - 417
VL  - 555
DO  - 10.4028/www.scientific.net/MSF.555.417
UR  - conv_1872
ER  - 
@conference{
author = "Obradović, Bojana and Osmokrović, A. and Bugarski, Branko and Bugarski, Diana and Vunjak-Novaković, Gordana",
year = "2007",
abstract = "Alginate was shown to be a suitable support for entrapment and cultivation of chondrocytes and bone marrow stromal cells, which under appropriate in vitro conditions synthesized cartilaginous components. The main limitation in these cultures may be low rates of mass transport through the alginate matrix governed by diffusion. In this study, we have designed and utilized a bioreactor system based on a packed bed of alginate beads with immobilized chondrogenic cells. Continuous medium perfusion provided convective mass transport through the packed bed, while small diameters of beads (2.5 mm and down to 500 mu m) ensured short diffusion distances to the immobilized cells. During up to 5 weeks of cultivation, the cells synthesized extracellular matrix components merging beads together and indicating potentials of this system for precise regulation of the cellular microenvironment in cartilage tissue engineering.",
publisher = "Trans Tech Publications Ltd, Durnten-Zurich",
journal = "Research Trends in Contemporary Materials Science",
title = "Alginate microbeads as a cell support for cartilage tissue engineering: Bioreactor studies",
pages = "+-417",
volume = "555",
doi = "10.4028/www.scientific.net/MSF.555.417",
url = "conv_1872"
}
Obradović, B., Osmokrović, A., Bugarski, B., Bugarski, D.,& Vunjak-Novaković, G.. (2007). Alginate microbeads as a cell support for cartilage tissue engineering: Bioreactor studies. in Research Trends in Contemporary Materials Science
Trans Tech Publications Ltd, Durnten-Zurich., 555, 417-+.
https://doi.org/10.4028/www.scientific.net/MSF.555.417
conv_1872
Obradović B, Osmokrović A, Bugarski B, Bugarski D, Vunjak-Novaković G. Alginate microbeads as a cell support for cartilage tissue engineering: Bioreactor studies. in Research Trends in Contemporary Materials Science. 2007;555:417-+.
doi:10.4028/www.scientific.net/MSF.555.417
conv_1872 .
Obradović, Bojana, Osmokrović, A., Bugarski, Branko, Bugarski, Diana, Vunjak-Novaković, Gordana, "Alginate microbeads as a cell support for cartilage tissue engineering: Bioreactor studies" in Research Trends in Contemporary Materials Science, 555 (2007):417-+,
https://doi.org/10.4028/www.scientific.net/MSF.555.417 .,
conv_1872 .

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