Despite its capacity for sexual reproduction and global distribution, Toxoplasma gondii has a highly clonal structure, with the majority of isolates belonging to three distinct clonal types. Congenital toxoplasmosis has been associated with type I and type II strains. We here present the first characterization of a T. gondii strain (BGD1) from South-East Europe, isolated from the umbilical blood of a 24-week-old fetus in Serbia. Genotyping, performed by PCR-RFLP using a set of nested PCR markers (5'SAG2, 3'SAG2, BTUB, SAG3, and GRA6), showed that the BGD1 strain possessed a type II genotype. The cytokine patterns in Swiss-Webster mice inoculated with brain cysts of BGD1 and the prototype type II ME49 strain were similar until 180 days post-infection, with highly elevated IFN-gamma, IL-12 and IL-10 by d7 and decreasing thereafter. While both strains induced pneumonia and hepatitis in acute infection (d14), chronic infection (d56) was characterized, in addition to hepatitis, by severe me...ningoencephalitis, associated with numerous brain cysts. Thus, the BGD1 strain of T. gondii has type II genotypic and immunologic characteristics, but unlike other type II strains of human origin, induces severe encephalitis, making it an alternative to the sheep-derived ME49 strain for experimental models of infection.
Keywords:
Toxoplasma gondii / congenital toxoplasmosis / genotyping / PCR-RFLP / type II strains / murine infection / cytokines / histopathology
Source:
Microbes & Infection, 2006, 8, 8, 2206-2212
Publisher:
Elsevier Science Bv, Amsterdam
Funding / projects:
United States Department of Health & Human Services, National Institutes of Health (NIH) - USANIH National Institute of Allergy & Infectious Diseases (NIAID) [AI059176, R01 AI059176] Funding Source: Medline
United States Department of Health & Human Services, National Institutes of Health (NIH) - USANIH National Institute of Allergy & Infectious Diseases (NIAID) [R01AI059176, R01AI059176, R01AI059176, R01AI0
TY - JOUR
AU - Đurković-Đaković, Olgica
AU - Klun, Ivana
AU - Khan, Asis
AU - Nikolić, Aleksandra
AU - Knežević-Usaj, Slavica
AU - Bobić, Branko
AU - Sibley, L. David
PY - 2006
UR - http://rimi.imi.bg.ac.rs/handle/123456789/152
AB - Despite its capacity for sexual reproduction and global distribution, Toxoplasma gondii has a highly clonal structure, with the majority of isolates belonging to three distinct clonal types. Congenital toxoplasmosis has been associated with type I and type II strains. We here present the first characterization of a T. gondii strain (BGD1) from South-East Europe, isolated from the umbilical blood of a 24-week-old fetus in Serbia. Genotyping, performed by PCR-RFLP using a set of nested PCR markers (5'SAG2, 3'SAG2, BTUB, SAG3, and GRA6), showed that the BGD1 strain possessed a type II genotype. The cytokine patterns in Swiss-Webster mice inoculated with brain cysts of BGD1 and the prototype type II ME49 strain were similar until 180 days post-infection, with highly elevated IFN-gamma, IL-12 and IL-10 by d7 and decreasing thereafter. While both strains induced pneumonia and hepatitis in acute infection (d14), chronic infection (d56) was characterized, in addition to hepatitis, by severe meningoencephalitis, associated with numerous brain cysts. Thus, the BGD1 strain of T. gondii has type II genotypic and immunologic characteristics, but unlike other type II strains of human origin, induces severe encephalitis, making it an alternative to the sheep-derived ME49 strain for experimental models of infection.
PB - Elsevier Science Bv, Amsterdam
T2 - Microbes & Infection
T1 - A human origin type II strain of Toxoplasma gondii causing severe encephalitis in mice
EP - 2212
IS - 8
SP - 2206
VL - 8
DO - 10.1016/j.micinf.2006.04.016
UR - conv_1767
ER -
@article{
author = "Đurković-Đaković, Olgica and Klun, Ivana and Khan, Asis and Nikolić, Aleksandra and Knežević-Usaj, Slavica and Bobić, Branko and Sibley, L. David",
year = "2006",
abstract = "Despite its capacity for sexual reproduction and global distribution, Toxoplasma gondii has a highly clonal structure, with the majority of isolates belonging to three distinct clonal types. Congenital toxoplasmosis has been associated with type I and type II strains. We here present the first characterization of a T. gondii strain (BGD1) from South-East Europe, isolated from the umbilical blood of a 24-week-old fetus in Serbia. Genotyping, performed by PCR-RFLP using a set of nested PCR markers (5'SAG2, 3'SAG2, BTUB, SAG3, and GRA6), showed that the BGD1 strain possessed a type II genotype. The cytokine patterns in Swiss-Webster mice inoculated with brain cysts of BGD1 and the prototype type II ME49 strain were similar until 180 days post-infection, with highly elevated IFN-gamma, IL-12 and IL-10 by d7 and decreasing thereafter. While both strains induced pneumonia and hepatitis in acute infection (d14), chronic infection (d56) was characterized, in addition to hepatitis, by severe meningoencephalitis, associated with numerous brain cysts. Thus, the BGD1 strain of T. gondii has type II genotypic and immunologic characteristics, but unlike other type II strains of human origin, induces severe encephalitis, making it an alternative to the sheep-derived ME49 strain for experimental models of infection.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Microbes & Infection",
title = "A human origin type II strain of Toxoplasma gondii causing severe encephalitis in mice",
pages = "2212-2206",
number = "8",
volume = "8",
doi = "10.1016/j.micinf.2006.04.016",
url = "conv_1767"
}
Đurković-Đaković, O., Klun, I., Khan, A., Nikolić, A., Knežević-Usaj, S., Bobić, B.,& Sibley, L. D.. (2006). A human origin type II strain of Toxoplasma gondii causing severe encephalitis in mice. in Microbes & Infection
Elsevier Science Bv, Amsterdam., 8(8), 2206-2212.
https://doi.org/10.1016/j.micinf.2006.04.016
conv_1767
Đurković-Đaković O, Klun I, Khan A, Nikolić A, Knežević-Usaj S, Bobić B, Sibley LD. A human origin type II strain of Toxoplasma gondii causing severe encephalitis in mice. in Microbes & Infection. 2006;8(8):2206-2212.
doi:10.1016/j.micinf.2006.04.016
conv_1767 .
Đurković-Đaković, Olgica, Klun, Ivana, Khan, Asis, Nikolić, Aleksandra, Knežević-Usaj, Slavica, Bobić, Branko, Sibley, L. David, "A human origin type II strain of Toxoplasma gondii causing severe encephalitis in mice" in Microbes & Infection, 8, no. 8 (2006):2206-2212,
https://doi.org/10.1016/j.micinf.2006.04.016 .,
conv_1767 .
