Immobilization of penicillin acylase from Escherichia coli on commercial Sepabeads carrier
Samo za registrovane korisnike
2007
Konferencijski prilog (Objavljena verzija)
,
Univerzitet u Nišu, Tehnološki fakultet Leskovac
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
This paper reports the covalent immobilization of penicillin G acylase from E. coli on sepabeads EC-EP, an epoxy-activated polymethacrylic carrier, and describes kinetic properties of the immobilized enzyme. The selected enzyme belongs to a class of biocatalysts whose industrial interest is due to their versatility to mediate hydrolysis of penicillins and semi-synthetic β−lactam antibiotics synthesis reactions. It appears that both free and immobilized penicillin acylase followed simple Michaelis-Menten kinetics, implying the same reaction mechanism in both systems.
Izvor:
VII Simpozijum sa međunarodnim učešćem "Savremene tehnologije i privredni razvoj", Leskovac, 19-20. oktobar, 2007, Zbornik radova, 2007, 16, BIH-1/BE-1, 33-42Izdavač:
- Univerzitet u Nišu, Tehnološki fakultet Leskovac
Institucija/grupa
Institut za medicinska istraživanjaTY - CONF AU - Žuža, Milena AU - Šiler-Marinković, Slavica AU - Knežević, Zorica PY - 2007 UR - http://rimi.imi.bg.ac.rs/handle/123456789/1367 AB - This paper reports the covalent immobilization of penicillin G acylase from E. coli on sepabeads EC-EP, an epoxy-activated polymethacrylic carrier, and describes kinetic properties of the immobilized enzyme. The selected enzyme belongs to a class of biocatalysts whose industrial interest is due to their versatility to mediate hydrolysis of penicillins and semi-synthetic β−lactam antibiotics synthesis reactions. It appears that both free and immobilized penicillin acylase followed simple Michaelis-Menten kinetics, implying the same reaction mechanism in both systems. PB - Univerzitet u Nišu, Tehnološki fakultet Leskovac C3 - VII Simpozijum sa međunarodnim učešćem "Savremene tehnologije i privredni razvoj", Leskovac, 19-20. oktobar, 2007, Zbornik radova T1 - Immobilization of penicillin acylase from Escherichia coli on commercial Sepabeads carrier EP - 42 IS - BIH-1/BE-1 SP - 33 VL - 16 UR - https://hdl.handle.net/21.15107/rcub_rimi_1367 ER -
@conference{ author = "Žuža, Milena and Šiler-Marinković, Slavica and Knežević, Zorica", year = "2007", abstract = "This paper reports the covalent immobilization of penicillin G acylase from E. coli on sepabeads EC-EP, an epoxy-activated polymethacrylic carrier, and describes kinetic properties of the immobilized enzyme. The selected enzyme belongs to a class of biocatalysts whose industrial interest is due to their versatility to mediate hydrolysis of penicillins and semi-synthetic β−lactam antibiotics synthesis reactions. It appears that both free and immobilized penicillin acylase followed simple Michaelis-Menten kinetics, implying the same reaction mechanism in both systems.", publisher = "Univerzitet u Nišu, Tehnološki fakultet Leskovac", journal = "VII Simpozijum sa međunarodnim učešćem "Savremene tehnologije i privredni razvoj", Leskovac, 19-20. oktobar, 2007, Zbornik radova", title = "Immobilization of penicillin acylase from Escherichia coli on commercial Sepabeads carrier", pages = "42-33", number = "BIH-1/BE-1", volume = "16", url = "https://hdl.handle.net/21.15107/rcub_rimi_1367" }
Žuža, M., Šiler-Marinković, S.,& Knežević, Z.. (2007). Immobilization of penicillin acylase from Escherichia coli on commercial Sepabeads carrier. in VII Simpozijum sa međunarodnim učešćem "Savremene tehnologije i privredni razvoj", Leskovac, 19-20. oktobar, 2007, Zbornik radova Univerzitet u Nišu, Tehnološki fakultet Leskovac., 16(BIH-1/BE-1), 33-42. https://hdl.handle.net/21.15107/rcub_rimi_1367
Žuža M, Šiler-Marinković S, Knežević Z. Immobilization of penicillin acylase from Escherichia coli on commercial Sepabeads carrier. in VII Simpozijum sa međunarodnim učešćem "Savremene tehnologije i privredni razvoj", Leskovac, 19-20. oktobar, 2007, Zbornik radova. 2007;16(BIH-1/BE-1):33-42. https://hdl.handle.net/21.15107/rcub_rimi_1367 .
Žuža, Milena, Šiler-Marinković, Slavica, Knežević, Zorica, "Immobilization of penicillin acylase from Escherichia coli on commercial Sepabeads carrier" in VII Simpozijum sa međunarodnim učešćem "Savremene tehnologije i privredni razvoj", Leskovac, 19-20. oktobar, 2007, Zbornik radova, 16, no. BIH-1/BE-1 (2007):33-42, https://hdl.handle.net/21.15107/rcub_rimi_1367 .