Disturbed Plasma Lipidomic Profiles in Females with Diffuse Large B-Cell Lymphoma: A Pilot Study
Authors
Masnikosa, Romana
Pirić, David
Post, Julia Maria
Cvetković, Zorica

Petrović, Snježana

Paunović, Marija

Vučić, Vesna

Bindila, Laura

Article (Published version)
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Lipidome dysregulation is a hallmark of cancer and inflammation. The global plasma lipidome and sub-lipidome of inflammatory pathways have not been reported in diffuse large B-cell lymphoma (DLBCL). In a pilot study of plasma lipid variation in female DLBCL patients and BMI-matched disease-free controls, we performed targeted lipidomics using LC-MRM to quantify lipid mediators of inflammation and immunity, and those known or hypothesised to be involved in cancer progression: sphingolipids, resolvin D1, arachidonic acid (AA)-derived oxylipins, such as hydroxyeicosatetraenoic acids (HETEs) and dihydroxyeicosatrienoic acids, along with their membrane structural precursors. We report on the role of the eicosanoids in the separation of DLBCL from controls, along with lysophosphatidylinositol LPI 20:4, implying notable changes in lipid metabolic and/or signalling pathways, particularly pertaining to AA lipoxygenase pathway and glycerophospholipid remodelling in the cell membrane. We suggest ...here the set of S1P, SM 36:1, SM 34:1 and PI 34:1 as DLBCL lipid signatures which could serve as a basis for the prospective validation in larger DLBCL cohorts. Additionally, untargeted lipidomics indicates a substantial change in the overall lipid metabolism in DLBCL. The plasma lipid profiling of DLBCL patients helps to better understand the specific lipid dysregulations and pathways in this cancer.
Keywords:
DLBCL / plasma 4D lipidomics / eicosanoids / HETEs / glycerophospholipids / sphingosine 1-phosphate / sphingolipids / targeted LC-MS / tims-TofSource:
Cancers, 2023, 15, 14, 3653-Funding / projects:
- Clinical Lipidomics Unit’s Core Facility at the Institute of Physiological Chemistry, University Medical Centre Mainz
- Germany Research Society grant number Bl 1399/2-1
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200017 (University of Belgrade, Institute of Nuclear Sciences 'Vinča', Belgrade-Vinča) (RS-200017)
- MBF-funded DIASyM, grant number 031 L0217
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Institut za medicinska istraživanjaTY - JOUR AU - Masnikosa, Romana AU - Pirić, David AU - Post, Julia Maria AU - Cvetković, Zorica AU - Petrović, Snježana AU - Paunović, Marija AU - Vučić, Vesna AU - Bindila, Laura PY - 2023 UR - http://rimi.imi.bg.ac.rs/handle/123456789/1340 AB - Lipidome dysregulation is a hallmark of cancer and inflammation. The global plasma lipidome and sub-lipidome of inflammatory pathways have not been reported in diffuse large B-cell lymphoma (DLBCL). In a pilot study of plasma lipid variation in female DLBCL patients and BMI-matched disease-free controls, we performed targeted lipidomics using LC-MRM to quantify lipid mediators of inflammation and immunity, and those known or hypothesised to be involved in cancer progression: sphingolipids, resolvin D1, arachidonic acid (AA)-derived oxylipins, such as hydroxyeicosatetraenoic acids (HETEs) and dihydroxyeicosatrienoic acids, along with their membrane structural precursors. We report on the role of the eicosanoids in the separation of DLBCL from controls, along with lysophosphatidylinositol LPI 20:4, implying notable changes in lipid metabolic and/or signalling pathways, particularly pertaining to AA lipoxygenase pathway and glycerophospholipid remodelling in the cell membrane. We suggest here the set of S1P, SM 36:1, SM 34:1 and PI 34:1 as DLBCL lipid signatures which could serve as a basis for the prospective validation in larger DLBCL cohorts. Additionally, untargeted lipidomics indicates a substantial change in the overall lipid metabolism in DLBCL. The plasma lipid profiling of DLBCL patients helps to better understand the specific lipid dysregulations and pathways in this cancer. T2 - Cancers T2 - Cancers T1 - Disturbed Plasma Lipidomic Profiles in Females with Diffuse Large B-Cell Lymphoma: A Pilot Study IS - 14 SP - 3653 VL - 15 DO - 10.3390/cancers15143653 ER -
@article{ author = "Masnikosa, Romana and Pirić, David and Post, Julia Maria and Cvetković, Zorica and Petrović, Snježana and Paunović, Marija and Vučić, Vesna and Bindila, Laura", year = "2023", abstract = "Lipidome dysregulation is a hallmark of cancer and inflammation. The global plasma lipidome and sub-lipidome of inflammatory pathways have not been reported in diffuse large B-cell lymphoma (DLBCL). In a pilot study of plasma lipid variation in female DLBCL patients and BMI-matched disease-free controls, we performed targeted lipidomics using LC-MRM to quantify lipid mediators of inflammation and immunity, and those known or hypothesised to be involved in cancer progression: sphingolipids, resolvin D1, arachidonic acid (AA)-derived oxylipins, such as hydroxyeicosatetraenoic acids (HETEs) and dihydroxyeicosatrienoic acids, along with their membrane structural precursors. We report on the role of the eicosanoids in the separation of DLBCL from controls, along with lysophosphatidylinositol LPI 20:4, implying notable changes in lipid metabolic and/or signalling pathways, particularly pertaining to AA lipoxygenase pathway and glycerophospholipid remodelling in the cell membrane. We suggest here the set of S1P, SM 36:1, SM 34:1 and PI 34:1 as DLBCL lipid signatures which could serve as a basis for the prospective validation in larger DLBCL cohorts. Additionally, untargeted lipidomics indicates a substantial change in the overall lipid metabolism in DLBCL. The plasma lipid profiling of DLBCL patients helps to better understand the specific lipid dysregulations and pathways in this cancer.", journal = "Cancers, Cancers", title = "Disturbed Plasma Lipidomic Profiles in Females with Diffuse Large B-Cell Lymphoma: A Pilot Study", number = "14", pages = "3653", volume = "15", doi = "10.3390/cancers15143653" }
Masnikosa, R., Pirić, D., Post, J. M., Cvetković, Z., Petrović, S., Paunović, M., Vučić, V.,& Bindila, L.. (2023). Disturbed Plasma Lipidomic Profiles in Females with Diffuse Large B-Cell Lymphoma: A Pilot Study. in Cancers, 15(14), 3653. https://doi.org/10.3390/cancers15143653
Masnikosa R, Pirić D, Post JM, Cvetković Z, Petrović S, Paunović M, Vučić V, Bindila L. Disturbed Plasma Lipidomic Profiles in Females with Diffuse Large B-Cell Lymphoma: A Pilot Study. in Cancers. 2023;15(14):3653. doi:10.3390/cancers15143653 .
Masnikosa, Romana, Pirić, David, Post, Julia Maria, Cvetković, Zorica, Petrović, Snježana, Paunović, Marija, Vučić, Vesna, Bindila, Laura, "Disturbed Plasma Lipidomic Profiles in Females with Diffuse Large B-Cell Lymphoma: A Pilot Study" in Cancers, 15, no. 14 (2023):3653, https://doi.org/10.3390/cancers15143653 . .