Chemical characterization and antimicrobial activity of Juglans nigra L. nut and green husk
Authors
Rajković, Katarina M.
Drobac, Milica

Milić, Petar
Vučić, Vesna

Arsić, Aleksandra

Perić, Mirijana

Radunović, Milena

Jeremić, Sanja

Arsenijević, Jelena

Article (Published version)
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Juglans nigra (Black walnut) is a source of health-supporting biologically active compounds used in traditional medicine. The investigation of bioactive compounds in black walnut could lead to its broader application, as well as to the application of its by-products. Therefore, this study aimed to characterize J. nigra nut and green husk based on chemical analysis of their petroleum ether and ethanol extracts obtained by ultrasonic and reflux extraction methods, respectively. Different extract fractions were tested for their antimicrobial activities using Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa), Gram-positive bacteria (Enterococcus faecalis, Staphylococcus aureus) and yeast (reference strain and clinical isolates of Candida albicans). The ethanol extracts analysis, performed by high performance liquid chromatography, singled out the ellagic acid as the most dominant compound in nut ((55.0±1.3)×10-3 kg m-3) and green husk ((114.1±0.5)×10-3 kg m-3) extracts. Non...-polar compounds were evaluated using gas chromatography analysis of petroleum ether extracts. Juglans nigra nut and green husk contained two saturated fatty acids, palmitic acid (C16:0) and stearic acid (C18:0), then, monounsaturated fatty acids, palmitoleic (C16:1n-7), oleic (C18:1n-9) and vaccenic acid (C18:1n-7), as well as polyunsaturated fatty acids, linoleic (C18:2n- -6), γ-linolenic (C18:3n-6) and α-linolenic (C18:3n-3) acids. Ethanol extracts of both J. nigra nut and green husk showed antimicrobial activity against C. albicans, which is the most common cause of yeast infections.
Keywords:
black walnut / Candida albicans / ellagic acid / fatty acidsSource:
Journal of the Serbian Chemical Society, 2023, 88, 6, 603-614Publisher:
- Serbian Chemical Society
Funding / projects:
- Ministry of Science, Technology, Development and Innovation of the Republic of Serbia (Grant Nos. 0702301)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200015 (University of Belgrade, Institute for Medical Research) (RS-200015)
- FungalCaseFinder - Prediction, prevention and patient's participation in diagnosis of selected fungal infections (FI): An implementation of novel method for obtaining tissue specimens (RS-7754282)
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Institut za medicinska istraživanjaTY - JOUR AU - Rajković, Katarina M. AU - Drobac, Milica AU - Milić, Petar AU - Vučić, Vesna AU - Arsić, Aleksandra AU - Perić, Mirijana AU - Radunović, Milena AU - Jeremić, Sanja AU - Arsenijević, Jelena PY - 2023 UR - http://rimi.imi.bg.ac.rs/handle/123456789/1322 AB - Juglans nigra (Black walnut) is a source of health-supporting biologically active compounds used in traditional medicine. The investigation of bioactive compounds in black walnut could lead to its broader application, as well as to the application of its by-products. Therefore, this study aimed to characterize J. nigra nut and green husk based on chemical analysis of their petroleum ether and ethanol extracts obtained by ultrasonic and reflux extraction methods, respectively. Different extract fractions were tested for their antimicrobial activities using Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa), Gram-positive bacteria (Enterococcus faecalis, Staphylococcus aureus) and yeast (reference strain and clinical isolates of Candida albicans). The ethanol extracts analysis, performed by high performance liquid chromatography, singled out the ellagic acid as the most dominant compound in nut ((55.0±1.3)×10-3 kg m-3) and green husk ((114.1±0.5)×10-3 kg m-3) extracts. Non-polar compounds were evaluated using gas chromatography analysis of petroleum ether extracts. Juglans nigra nut and green husk contained two saturated fatty acids, palmitic acid (C16:0) and stearic acid (C18:0), then, monounsaturated fatty acids, palmitoleic (C16:1n-7), oleic (C18:1n-9) and vaccenic acid (C18:1n-7), as well as polyunsaturated fatty acids, linoleic (C18:2n- -6), γ-linolenic (C18:3n-6) and α-linolenic (C18:3n-3) acids. Ethanol extracts of both J. nigra nut and green husk showed antimicrobial activity against C. albicans, which is the most common cause of yeast infections. PB - Serbian Chemical Society T2 - Journal of the Serbian Chemical Society T2 - Journal of the Serbian Chemical Society T1 - Chemical characterization and antimicrobial activity of Juglans nigra L. nut and green husk EP - 614 IS - 6 SP - 603 VL - 88 DO - 10.2298/JSC230210024R ER -
@article{ author = "Rajković, Katarina M. and Drobac, Milica and Milić, Petar and Vučić, Vesna and Arsić, Aleksandra and Perić, Mirijana and Radunović, Milena and Jeremić, Sanja and Arsenijević, Jelena", year = "2023", abstract = "Juglans nigra (Black walnut) is a source of health-supporting biologically active compounds used in traditional medicine. The investigation of bioactive compounds in black walnut could lead to its broader application, as well as to the application of its by-products. Therefore, this study aimed to characterize J. nigra nut and green husk based on chemical analysis of their petroleum ether and ethanol extracts obtained by ultrasonic and reflux extraction methods, respectively. Different extract fractions were tested for their antimicrobial activities using Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa), Gram-positive bacteria (Enterococcus faecalis, Staphylococcus aureus) and yeast (reference strain and clinical isolates of Candida albicans). The ethanol extracts analysis, performed by high performance liquid chromatography, singled out the ellagic acid as the most dominant compound in nut ((55.0±1.3)×10-3 kg m-3) and green husk ((114.1±0.5)×10-3 kg m-3) extracts. Non-polar compounds were evaluated using gas chromatography analysis of petroleum ether extracts. Juglans nigra nut and green husk contained two saturated fatty acids, palmitic acid (C16:0) and stearic acid (C18:0), then, monounsaturated fatty acids, palmitoleic (C16:1n-7), oleic (C18:1n-9) and vaccenic acid (C18:1n-7), as well as polyunsaturated fatty acids, linoleic (C18:2n- -6), γ-linolenic (C18:3n-6) and α-linolenic (C18:3n-3) acids. Ethanol extracts of both J. nigra nut and green husk showed antimicrobial activity against C. albicans, which is the most common cause of yeast infections.", publisher = "Serbian Chemical Society", journal = "Journal of the Serbian Chemical Society, Journal of the Serbian Chemical Society", title = "Chemical characterization and antimicrobial activity of Juglans nigra L. nut and green husk", pages = "614-603", number = "6", volume = "88", doi = "10.2298/JSC230210024R" }
Rajković, K. M., Drobac, M., Milić, P., Vučić, V., Arsić, A., Perić, M., Radunović, M., Jeremić, S.,& Arsenijević, J.. (2023). Chemical characterization and antimicrobial activity of Juglans nigra L. nut and green husk. in Journal of the Serbian Chemical Society Serbian Chemical Society., 88(6), 603-614. https://doi.org/10.2298/JSC230210024R
Rajković KM, Drobac M, Milić P, Vučić V, Arsić A, Perić M, Radunović M, Jeremić S, Arsenijević J. Chemical characterization and antimicrobial activity of Juglans nigra L. nut and green husk. in Journal of the Serbian Chemical Society. 2023;88(6):603-614. doi:10.2298/JSC230210024R .
Rajković, Katarina M., Drobac, Milica, Milić, Petar, Vučić, Vesna, Arsić, Aleksandra, Perić, Mirijana, Radunović, Milena, Jeremić, Sanja, Arsenijević, Jelena, "Chemical characterization and antimicrobial activity of Juglans nigra L. nut and green husk" in Journal of the Serbian Chemical Society, 88, no. 6 (2023):603-614, https://doi.org/10.2298/JSC230210024R . .