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The effects of endothelin and L-NAME on plasma uric acid and urea concentration in Wistar rats

Uticaj endotelina i L-NAME na koncentraciju mokraćne kiseline i uree u plazmi kod Wistar pacova

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2006
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Authors
Popović, Tamara B.
Jovović, Đurđica
Miloradović, Zoran
Mihailović-Stanojević, Nevena
Spasić, M.
Article (Published version)
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Abstract
Effects of endothelins, which include vasoconstriction and vasodilatation are possible by different molecular mechanisms. Endothelins induce increased synthesis of nitric oxide which in turn inhibits the synthesis of endothelin. Changes in biochemical parameters connected with the increased and decreased synthesis of nitric oxide are not thoroughly examined yet. In a series of experiments we compared the effects of endothelin (ET-1) on the inhibition of nitric oxide synthase (NOS), as well as the effects of L-nitro arginin metal estar (L-NAME) on plasma urea and uric acid concentration. Experiments were performed on anesthetized female adult Wistar rats. The experimental groups received an i. v. bolus of ET-1, L-NAME or saline. All parameters, heamodynamic and biochemical, were measured before the animals were sacrificed. L-NAME increased the mean arterial pressure, decreased renal blood flow and increased vascular resistance in carotid and renal arteries and increased in plasma uric a...cid and urea concentrations. ET- 1 significantly decreased uric acid concentration in the plasma but did not effect plasma urea concentration compared to the control group. These differences show complex relations of nitric oxide with cellular signalization compared to the basic NO-cGMP pathway.

Efekti endotelina koji uključuju vazokonstrikciju I vazodilataciju ostvaruju se različitim molekularnim mehanizmima. Endotelin indukuje povećanu sintezu azotnog oksida za koji se smatra da povratno inhibira sintezu endotelina. Promene biohemijskih parametara vezanih za povećanu i smanjenu sintezu NO nisu dovoljno proučene. U ovom radu su upoređeni efekti endotelina (ET-1) i inhibitora NOS-a, L-NAME na koncentraciju mokraćne kiseline i uree u plazmi. Eksperimenti su izvedeni na anesteziranim odraslim NW pacovima, ženskog pola. Eksperimentalne životinje su u i.v. bolusu primale ET-1, L-NAME ili fiziološki rastvor. Svi hemodinamski i biohemijski parametri su mereni pre žrtvovanja. L-NAME dovodi do povećanja srednjeg arterijskog pritiska smanjenja renalnog protoka i povećanja vaskularnog otpora u karotidnoj i renalnoj arteriji i povećanja koncentracije mokraćne kiseline i uree u plazmi. ET-1 značajno smanjuje koncentraciju mokraćne kiseline u plazmi ali ne utiče na koncentraciju uree u odn...osu na kontrolu. Ove razlike pokazuju kompleksnije relacije NO-a sa ćelijskom signalizacijom u odnosu na osnovni NO-cGMP put.

Keywords:
endothelin / L-NAME / haemodynamic / uric acid / urea / rats
Source:
Acta Veterinaria-Beograd, 2006, 56, 2-3, 181-189
Publisher:
  • Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd

DOI: 10.2298/AVB0603181P

ISSN: 0567-8315

WoS: 000238491800008

Scopus: 2-s2.0-33745881947
[ Google Scholar ]
URI
http://rimi.imi.bg.ac.rs/handle/123456789/132
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
Institut za medicinska istraživanja
TY  - JOUR
AU  - Popović, Tamara B.
AU  - Jovović, Đurđica
AU  - Miloradović, Zoran
AU  - Mihailović-Stanojević, Nevena
AU  - Spasić, M.
PY  - 2006
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/132
AB  - Effects of endothelins, which include vasoconstriction and vasodilatation are possible by different molecular mechanisms. Endothelins induce increased synthesis of nitric oxide which in turn inhibits the synthesis of endothelin. Changes in biochemical parameters connected with the increased and decreased synthesis of nitric oxide are not thoroughly examined yet. In a series of experiments we compared the effects of endothelin (ET-1) on the inhibition of nitric oxide synthase (NOS), as well as the effects of L-nitro arginin metal estar (L-NAME) on plasma urea and uric acid concentration. Experiments were performed on anesthetized female adult Wistar rats. The experimental groups received an i. v. bolus of ET-1, L-NAME or saline. All parameters, heamodynamic and biochemical, were measured before the animals were sacrificed. L-NAME increased the mean arterial pressure, decreased renal blood flow and increased vascular resistance in carotid and renal arteries and increased in plasma uric acid and urea concentrations. ET- 1 significantly decreased uric acid concentration in the plasma but did not effect plasma urea concentration compared to the control group. These differences show complex relations of nitric oxide with cellular signalization compared to the basic NO-cGMP pathway.
AB  - Efekti endotelina koji uključuju vazokonstrikciju I vazodilataciju ostvaruju se različitim molekularnim mehanizmima. Endotelin indukuje povećanu sintezu azotnog oksida za koji se smatra da povratno inhibira sintezu endotelina. Promene biohemijskih parametara vezanih za povećanu i smanjenu sintezu NO nisu dovoljno proučene. U ovom radu su upoređeni efekti endotelina (ET-1) i inhibitora NOS-a, L-NAME na koncentraciju mokraćne kiseline i uree u plazmi. Eksperimenti su izvedeni na anesteziranim odraslim NW pacovima, ženskog pola. Eksperimentalne životinje su u i.v. bolusu primale ET-1, L-NAME ili fiziološki rastvor. Svi hemodinamski i biohemijski parametri su mereni pre žrtvovanja. L-NAME dovodi do povećanja srednjeg arterijskog pritiska smanjenja renalnog protoka i povećanja vaskularnog otpora u karotidnoj i renalnoj arteriji i povećanja koncentracije mokraćne kiseline i uree u plazmi. ET-1 značajno smanjuje koncentraciju mokraćne kiseline u plazmi ali ne utiče na koncentraciju uree u odnosu na kontrolu. Ove razlike pokazuju kompleksnije relacije NO-a sa ćelijskom signalizacijom u odnosu na osnovni NO-cGMP put.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - The effects of endothelin and L-NAME on plasma uric acid and urea concentration in Wistar rats
T1  - Uticaj endotelina i L-NAME na koncentraciju mokraćne kiseline i uree u plazmi kod Wistar pacova
EP  - 189
IS  - 2-3
SP  - 181
VL  - 56
DO  - 10.2298/AVB0603181P
UR  - conv_1737
ER  - 
@article{
author = "Popović, Tamara B. and Jovović, Đurđica and Miloradović, Zoran and Mihailović-Stanojević, Nevena and Spasić, M.",
year = "2006",
abstract = "Effects of endothelins, which include vasoconstriction and vasodilatation are possible by different molecular mechanisms. Endothelins induce increased synthesis of nitric oxide which in turn inhibits the synthesis of endothelin. Changes in biochemical parameters connected with the increased and decreased synthesis of nitric oxide are not thoroughly examined yet. In a series of experiments we compared the effects of endothelin (ET-1) on the inhibition of nitric oxide synthase (NOS), as well as the effects of L-nitro arginin metal estar (L-NAME) on plasma urea and uric acid concentration. Experiments were performed on anesthetized female adult Wistar rats. The experimental groups received an i. v. bolus of ET-1, L-NAME or saline. All parameters, heamodynamic and biochemical, were measured before the animals were sacrificed. L-NAME increased the mean arterial pressure, decreased renal blood flow and increased vascular resistance in carotid and renal arteries and increased in plasma uric acid and urea concentrations. ET- 1 significantly decreased uric acid concentration in the plasma but did not effect plasma urea concentration compared to the control group. These differences show complex relations of nitric oxide with cellular signalization compared to the basic NO-cGMP pathway., Efekti endotelina koji uključuju vazokonstrikciju I vazodilataciju ostvaruju se različitim molekularnim mehanizmima. Endotelin indukuje povećanu sintezu azotnog oksida za koji se smatra da povratno inhibira sintezu endotelina. Promene biohemijskih parametara vezanih za povećanu i smanjenu sintezu NO nisu dovoljno proučene. U ovom radu su upoređeni efekti endotelina (ET-1) i inhibitora NOS-a, L-NAME na koncentraciju mokraćne kiseline i uree u plazmi. Eksperimenti su izvedeni na anesteziranim odraslim NW pacovima, ženskog pola. Eksperimentalne životinje su u i.v. bolusu primale ET-1, L-NAME ili fiziološki rastvor. Svi hemodinamski i biohemijski parametri su mereni pre žrtvovanja. L-NAME dovodi do povećanja srednjeg arterijskog pritiska smanjenja renalnog protoka i povećanja vaskularnog otpora u karotidnoj i renalnoj arteriji i povećanja koncentracije mokraćne kiseline i uree u plazmi. ET-1 značajno smanjuje koncentraciju mokraćne kiseline u plazmi ali ne utiče na koncentraciju uree u odnosu na kontrolu. Ove razlike pokazuju kompleksnije relacije NO-a sa ćelijskom signalizacijom u odnosu na osnovni NO-cGMP put.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "The effects of endothelin and L-NAME on plasma uric acid and urea concentration in Wistar rats, Uticaj endotelina i L-NAME na koncentraciju mokraćne kiseline i uree u plazmi kod Wistar pacova",
pages = "189-181",
number = "2-3",
volume = "56",
doi = "10.2298/AVB0603181P",
url = "conv_1737"
}
Popović, T. B., Jovović, Đ., Miloradović, Z., Mihailović-Stanojević, N.,& Spasić, M.. (2006). The effects of endothelin and L-NAME on plasma uric acid and urea concentration in Wistar rats. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 56(2-3), 181-189.
https://doi.org/10.2298/AVB0603181P
conv_1737
Popović TB, Jovović Đ, Miloradović Z, Mihailović-Stanojević N, Spasić M. The effects of endothelin and L-NAME on plasma uric acid and urea concentration in Wistar rats. in Acta Veterinaria-Beograd. 2006;56(2-3):181-189.
doi:10.2298/AVB0603181P
conv_1737 .
Popović, Tamara B., Jovović, Đurđica, Miloradović, Zoran, Mihailović-Stanojević, Nevena, Spasić, M., "The effects of endothelin and L-NAME on plasma uric acid and urea concentration in Wistar rats" in Acta Veterinaria-Beograd, 56, no. 2-3 (2006):181-189,
https://doi.org/10.2298/AVB0603181P .,
conv_1737 .

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