Autochthonous infection with Ehrlichia Canis and Hepatozoon Canis in dogs from Serbia
Authors
Sukara, Ratko
Andrić, Nenad

Francuski Andrić, Jelena
Mihaljica, Darko

Veinović, Gorana

Ranković, Vladan
Tomanović, Snežana

Article (Published version)
Metadata
Show full item recordAbstract
Abstract Background The epidemiological status concerning many canine tick-borne diseases (TBDs) in Serbia is still insufficiently known. Objectives Our study aimed to investigate the presence of tick-borne pathogens of the family Anaplasmataceae and Hepatozoon spp., as a cause of illnesses accompanied by clinical signs that can occur in dogs with anaplasmosis, ehrlichiosis and hepatozoonosis. Methods Dogs are included in the study based on the presence of a minimum of three clinical and/or pathological findings that could be associated with anaplasmosis, ehrlichiosis and hepatozoonosis. During the study (April–October 2018), 11 dogs met the conditions to be included in the survey. Identification of the causative agent in the blood of diseased dogs was performed by conventional PCR followed by sequencing. Results The presence of the pathogens was confirmed in three animals (3/11, 27.3%). The presence of Ehrlichia canis was confirmed in 3-month-old female Rottweiler puppy, an 8-year... old Miniature Schnauzer female was positive for Hepatozoon canis infection, while 4-year-old mixed breed male dog was co-infected with both mentioned pathogens. These are the first cases of autochthonous infection with E. canis and H. canis in dogs from Serbia confirmed by molecular methods. Conclusions The results of our study indicate the importance of molecular methods to establish a reliable diagnosis of TBDs. Also, the confirmed presence of causative agents of canine monocytic ehrlichiosis and hepatozoonosis in Serbia appeals to veterinary practitioners that it is necessary to exclude the presence of those diseases in suspicious dogs.
Keywords:
dogs / Ehrlichia canis / Hepatozoon canis / polymerase chain reaction / Serbia / tick-borne diseasesSource:
Veterinary Medicine and Science, 2023, 9, 1, 111-118Publisher:
- Wiley-Blackwell
Funding / projects:
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200015 (University of Belgrade, Institute for Medical Research) (RS-200015)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200143 (University of Belgrade, Faculty of Veterinary Medicine) (RS-200143)
Collections
Institution/Community
Institut za medicinska istraživanjaTY - JOUR AU - Sukara, Ratko AU - Andrić, Nenad AU - Francuski Andrić, Jelena AU - Mihaljica, Darko AU - Veinović, Gorana AU - Ranković, Vladan AU - Tomanović, Snežana PY - 2023 UR - http://rimi.imi.bg.ac.rs/handle/123456789/1270 AB - Abstract Background The epidemiological status concerning many canine tick-borne diseases (TBDs) in Serbia is still insufficiently known. Objectives Our study aimed to investigate the presence of tick-borne pathogens of the family Anaplasmataceae and Hepatozoon spp., as a cause of illnesses accompanied by clinical signs that can occur in dogs with anaplasmosis, ehrlichiosis and hepatozoonosis. Methods Dogs are included in the study based on the presence of a minimum of three clinical and/or pathological findings that could be associated with anaplasmosis, ehrlichiosis and hepatozoonosis. During the study (April–October 2018), 11 dogs met the conditions to be included in the survey. Identification of the causative agent in the blood of diseased dogs was performed by conventional PCR followed by sequencing. Results The presence of the pathogens was confirmed in three animals (3/11, 27.3%). The presence of Ehrlichia canis was confirmed in 3-month-old female Rottweiler puppy, an 8-year old Miniature Schnauzer female was positive for Hepatozoon canis infection, while 4-year-old mixed breed male dog was co-infected with both mentioned pathogens. These are the first cases of autochthonous infection with E. canis and H. canis in dogs from Serbia confirmed by molecular methods. Conclusions The results of our study indicate the importance of molecular methods to establish a reliable diagnosis of TBDs. Also, the confirmed presence of causative agents of canine monocytic ehrlichiosis and hepatozoonosis in Serbia appeals to veterinary practitioners that it is necessary to exclude the presence of those diseases in suspicious dogs. PB - Wiley-Blackwell T2 - Veterinary Medicine and Science T1 - Autochthonous infection with Ehrlichia Canis and Hepatozoon Canis in dogs from Serbia EP - 118 IS - 1 SP - 111 VL - 9 DO - 10.1002/vms3.1061 ER -
@article{ author = "Sukara, Ratko and Andrić, Nenad and Francuski Andrić, Jelena and Mihaljica, Darko and Veinović, Gorana and Ranković, Vladan and Tomanović, Snežana", year = "2023", abstract = "Abstract Background The epidemiological status concerning many canine tick-borne diseases (TBDs) in Serbia is still insufficiently known. Objectives Our study aimed to investigate the presence of tick-borne pathogens of the family Anaplasmataceae and Hepatozoon spp., as a cause of illnesses accompanied by clinical signs that can occur in dogs with anaplasmosis, ehrlichiosis and hepatozoonosis. Methods Dogs are included in the study based on the presence of a minimum of three clinical and/or pathological findings that could be associated with anaplasmosis, ehrlichiosis and hepatozoonosis. During the study (April–October 2018), 11 dogs met the conditions to be included in the survey. Identification of the causative agent in the blood of diseased dogs was performed by conventional PCR followed by sequencing. Results The presence of the pathogens was confirmed in three animals (3/11, 27.3%). The presence of Ehrlichia canis was confirmed in 3-month-old female Rottweiler puppy, an 8-year old Miniature Schnauzer female was positive for Hepatozoon canis infection, while 4-year-old mixed breed male dog was co-infected with both mentioned pathogens. These are the first cases of autochthonous infection with E. canis and H. canis in dogs from Serbia confirmed by molecular methods. Conclusions The results of our study indicate the importance of molecular methods to establish a reliable diagnosis of TBDs. Also, the confirmed presence of causative agents of canine monocytic ehrlichiosis and hepatozoonosis in Serbia appeals to veterinary practitioners that it is necessary to exclude the presence of those diseases in suspicious dogs.", publisher = "Wiley-Blackwell", journal = "Veterinary Medicine and Science", title = "Autochthonous infection with Ehrlichia Canis and Hepatozoon Canis in dogs from Serbia", pages = "118-111", number = "1", volume = "9", doi = "10.1002/vms3.1061" }
Sukara, R., Andrić, N., Francuski Andrić, J., Mihaljica, D., Veinović, G., Ranković, V.,& Tomanović, S.. (2023). Autochthonous infection with Ehrlichia Canis and Hepatozoon Canis in dogs from Serbia. in Veterinary Medicine and Science Wiley-Blackwell., 9(1), 111-118. https://doi.org/10.1002/vms3.1061
Sukara R, Andrić N, Francuski Andrić J, Mihaljica D, Veinović G, Ranković V, Tomanović S. Autochthonous infection with Ehrlichia Canis and Hepatozoon Canis in dogs from Serbia. in Veterinary Medicine and Science. 2023;9(1):111-118. doi:10.1002/vms3.1061 .
Sukara, Ratko, Andrić, Nenad, Francuski Andrić, Jelena, Mihaljica, Darko, Veinović, Gorana, Ranković, Vladan, Tomanović, Snežana, "Autochthonous infection with Ehrlichia Canis and Hepatozoon Canis in dogs from Serbia" in Veterinary Medicine and Science, 9, no. 1 (2023):111-118, https://doi.org/10.1002/vms3.1061 . .