RIMI - Repository of the Institute for Medical Research
Institute for Medical Research
    • English
    • Српски
    • Српски (Serbia)
  • English 
    • English
    • Serbian (Cyrillic)
    • Serbian (Latin)
  • Login
View Item 
  •   RIMI
  • Institut za medicinska istraživanja
  • Radovi istraživača / Researchers' publications
  • View Item
  •   RIMI
  • Institut za medicinska istraživanja
  • Radovi istraživača / Researchers' publications
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Grapefruit Juice Flavanones Modulate the Expression of Genes Regulating Inflammation, Cell Interactions and Vascular Function in Peripheral Blood Mononuclear Cells of Postmenopausal Women

Thumbnail
2022
Grapefruit_Juice_Flavanones_Modulate_the_Expression_pub_2022.pdf (7.952Mb)
Authors
Krga, Irena
Corral-Jara, Karla Fabiola
Barber-Chamoux, Nicolas
Dubray, Claude
Morand, Christine
Milenković, Dragan
Article (Published version)
Metadata
Show full item record
Abstract
Grapefruit is a rich source of flavanones, phytochemicals suggested excreting vasculoprotective effects. We previously showed that flavanones in grapefruit juice (GFJ) reduced postmenopausal women’s pulse-wave velocity (PWV), a measure of arterial stiffness. However, mechanisms of flavanone action in humans are largely unknown. This study aimed to decipher molecular mechanisms of flavanones by multi-omics analysis in PBMCs of volunteers consuming GFJ and flavanone-free control drink for 6 months. Modulated genes and microRNAs (miRNAs) were identified using microarrays. Bioinformatics analyses assessed their functions, interactions and correlations with previously observed changes in PWV. GFJ modified gene and miRNA expressions. Integrated analysis of modulated genes and miRNA-target genes suggests regulation of inflammation, immune response, cell interaction and mobility. Bioinformatics identified putative mediators of the observed nutrigenomic effect (STAT3, NF-κB) and mol...ecular docking demonstrated potential binding of flavanone metabolites to transcription factors and cell-signaling proteins. We also observed 34 significant correlations between changes in gene expression and PWV. Moreover, global gene expression was negatively correlated with gene expression profiles in arterial stiffness and hypertension. This study revealed molecular mechanisms underlying vasculoprotective effects of flavanones, including interactions with transcription factors and gene and miRNA expression changes that inversely correlate with gene expression profiles associated with cardiovascular risk factors.

Keywords:
naringin / flavanone / genomics / bioinformatics / vascular function
Source:
Frontiers in Nutrition, 2022, 9, 907595-
Publisher:
  • Frontiers Media S.A.
Funding / projects:
  • This work was supported by a grant from the Florida Department of Citrus.
  • Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200015 (University of Belgrade, Institute for Medical Research) (RS-200015)

DOI: 10.3389/fnut.2022.907595

ISSN: 2296-861X

[ Google Scholar ]
URI
http://rimi.imi.bg.ac.rs/handle/123456789/1243
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
Institut za medicinska istraživanja
TY  - JOUR
AU  - Krga, Irena
AU  - Corral-Jara, Karla Fabiola
AU  - Barber-Chamoux, Nicolas
AU  - Dubray, Claude
AU  - Morand, Christine
AU  - Milenković, Dragan
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1243
AB  - Grapefruit is a rich source of flavanones, phytochemicals suggested excreting
vasculoprotective effects. We previously showed that flavanones in grapefruit juice
(GFJ) reduced postmenopausal women’s pulse-wave velocity (PWV), a measure of
arterial stiffness. However, mechanisms of flavanone action in humans are largely
unknown. This study aimed to decipher molecular mechanisms of flavanones by
multi-omics analysis in PBMCs of volunteers consuming GFJ and flavanone-free
control drink for 6 months. Modulated genes and microRNAs (miRNAs) were identified
using microarrays. Bioinformatics analyses assessed their functions, interactions and
correlations with previously observed changes in PWV. GFJ modified gene and
miRNA expressions. Integrated analysis of modulated genes and miRNA-target genes
suggests regulation of inflammation, immune response, cell interaction and mobility.
Bioinformatics identified putative mediators of the observed nutrigenomic effect
(STAT3, NF-κB) and molecular docking demonstrated potential binding of flavanone
metabolites to transcription factors and cell-signaling proteins. We also observed 34
significant correlations between changes in gene expression and PWV. Moreover, global
gene expression was negatively correlated with gene expression profiles in arterial
stiffness and hypertension. This study revealed molecular mechanisms underlying
vasculoprotective effects of flavanones, including interactions with transcription factors
and gene and miRNA expression changes that inversely correlate with gene expression
profiles associated with cardiovascular risk factors.
PB  - Frontiers Media S.A.
T2  - Frontiers in Nutrition
T1  - Grapefruit Juice Flavanones Modulate the Expression of Genes Regulating Inflammation, Cell Interactions and Vascular Function in Peripheral Blood Mononuclear Cells of Postmenopausal Women
SP  - 907595
VL  - 9
VL  - 9
DO  - 10.3389/fnut.2022.907595
ER  - 
@article{
author = "Krga, Irena and Corral-Jara, Karla Fabiola and Barber-Chamoux, Nicolas and Dubray, Claude and Morand, Christine and Milenković, Dragan",
year = "2022",
abstract = "Grapefruit is a rich source of flavanones, phytochemicals suggested excreting
vasculoprotective effects. We previously showed that flavanones in grapefruit juice
(GFJ) reduced postmenopausal women’s pulse-wave velocity (PWV), a measure of
arterial stiffness. However, mechanisms of flavanone action in humans are largely
unknown. This study aimed to decipher molecular mechanisms of flavanones by
multi-omics analysis in PBMCs of volunteers consuming GFJ and flavanone-free
control drink for 6 months. Modulated genes and microRNAs (miRNAs) were identified
using microarrays. Bioinformatics analyses assessed their functions, interactions and
correlations with previously observed changes in PWV. GFJ modified gene and
miRNA expressions. Integrated analysis of modulated genes and miRNA-target genes
suggests regulation of inflammation, immune response, cell interaction and mobility.
Bioinformatics identified putative mediators of the observed nutrigenomic effect
(STAT3, NF-κB) and molecular docking demonstrated potential binding of flavanone
metabolites to transcription factors and cell-signaling proteins. We also observed 34
significant correlations between changes in gene expression and PWV. Moreover, global
gene expression was negatively correlated with gene expression profiles in arterial
stiffness and hypertension. This study revealed molecular mechanisms underlying
vasculoprotective effects of flavanones, including interactions with transcription factors
and gene and miRNA expression changes that inversely correlate with gene expression
profiles associated with cardiovascular risk factors.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Nutrition",
title = "Grapefruit Juice Flavanones Modulate the Expression of Genes Regulating Inflammation, Cell Interactions and Vascular Function in Peripheral Blood Mononuclear Cells of Postmenopausal Women",
pages = "907595",
volume = "9, 9",
doi = "10.3389/fnut.2022.907595"
}
Krga, I., Corral-Jara, K. F., Barber-Chamoux, N., Dubray, C., Morand, C.,& Milenković, D.. (2022). Grapefruit Juice Flavanones Modulate the Expression of Genes Regulating Inflammation, Cell Interactions and Vascular Function in Peripheral Blood Mononuclear Cells of Postmenopausal Women. in Frontiers in Nutrition
Frontiers Media S.A.., 9, 907595.
https://doi.org/10.3389/fnut.2022.907595
Krga I, Corral-Jara KF, Barber-Chamoux N, Dubray C, Morand C, Milenković D. Grapefruit Juice Flavanones Modulate the Expression of Genes Regulating Inflammation, Cell Interactions and Vascular Function in Peripheral Blood Mononuclear Cells of Postmenopausal Women. in Frontiers in Nutrition. 2022;9:907595.
doi:10.3389/fnut.2022.907595 .
Krga, Irena, Corral-Jara, Karla Fabiola, Barber-Chamoux, Nicolas, Dubray, Claude, Morand, Christine, Milenković, Dragan, "Grapefruit Juice Flavanones Modulate the Expression of Genes Regulating Inflammation, Cell Interactions and Vascular Function in Peripheral Blood Mononuclear Cells of Postmenopausal Women" in Frontiers in Nutrition, 9 (2022):907595,
https://doi.org/10.3389/fnut.2022.907595 . .

DSpace software copyright © 2002-2015  DuraSpace
About RIMI | Send Feedback

OpenAIRERCUB
 

 

All of DSpaceCommunitiesAuthorsTitlesSubjectsThis institutionAuthorsTitlesSubjects

Statistics

View Usage Statistics

DSpace software copyright © 2002-2015  DuraSpace
About RIMI | Send Feedback

OpenAIRERCUB