Приказ основних података о документу

dc.creatorVasić, Ana
dc.creatorRăileanu, Cristian
dc.creatorKörsten, Christin
dc.creatorVojinović, Dragica
dc.creatorManić, Marija
dc.creatorUrošević, Aleksandar
dc.creatorNikolić, Nataša
dc.creatorDulović, Olga
dc.creatorTews, Birke Andrea
dc.creatorPetrović, Tamaš
dc.creatorSilaghi, Cornelia
dc.creatorValčić, Miroslav
dc.creatorGligić, Ana
dc.date.accessioned2022-06-14T10:40:37Z
dc.date.available2022-06-14T10:40:37Z
dc.date.issued2022
dc.identifier.issn1865-1682
dc.identifier.issn1865-1674
dc.identifier.urihttp://rimi.imi.bg.ac.rs/handle/123456789/1238
dc.description.abstractWest Nile virus (WNV) is a zoonotic mosquito-borne virus classified as family Flaviviridae and genus Flavivirus. The first WNV outbreak in humans in the Republic of Serbia was recorded in 2012. Equids and dogs can show clinical symptoms after WNV infection and are often used as sentinels. This study aimed to (i) give insight into seropositivity for WNV in clinically healthy dog and horse sera in different regions of Serbia and (ii) compare diagnostic value of ‘in-house’ and commercially available indirect immunofluorescence (IFA) and enzyme-linked immunoassay (ELISA) tests to ‘gold standard’ virus neutralization test (VNT). Due to cross-reactivity, sera were tested for Usutu virus and tick-borne encephalitis virus in VNT based on the epidemiological data of field presence. Blood sera of dogs (n = 184) and horses (n = 232) were collected from 2011 to 2013. The seropositivity was confirmed by VNT in 36.9 % tested dog sera and 34.9% tested horse sera with highest positivity in regions near two big rivers, while in four dog and seven horse sera, positivity resulted from Usutu virus infection. Comparative results of diagnostic tests in dogs ranged from 18.7 % seropositivity by ‘in-house’ ELISA to 31.9% by commercially available ELISA. In horses, seropositivity ranged from 36.2% by ‘in-house’ IFA to 32.5% by commercially available IFA and from 26.3% by ‘in-house’ IgG ELISA to 20.9% by commercially available ELISA. There were no statistically significant differences according to the McNemar test between ‘in-house’ and commercially available IFA and ELISA test in horse sera, while the same was not true for two ELISAs used in dog sera (χ2 = 8.647, p = .003). Established seropositivity in dogs and horses was in accordance with the epidemiological situation and WNV spread in the Republic of Serbia and proven Usutu virus co-circulation. ‘In-house’ tests remain a valuable tool in early diagnostics of WNV.
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/37015/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200015/RS//
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceTransboundary and Emerging Diseases
dc.subjectdogs
dc.subjecthorses
dc.subjectSerbia
dc.subjectserology
dc.subjectWest Nile virus
dc.titleWest Nile virus in the Republic of Serbia—Diagnostic performance of five serological tests in dog and horse sera
dc.typearticle
dc.rights.licenseBY-NC-ND
dc.citation.epagee2515
dc.citation.issue5
dc.citation.spagee2506
dc.citation.volume69
dc.identifier.doi10.1111/tbed.14593
dc.identifier.fulltexthttp://rimi.imi.bg.ac.rs/bitstream/id/2752/West_Nile_virus_in_the_Republic_of_Serbia_Diagnostic_pub_2022.pdf
dc.type.versionpublishedVersion


Документи

Thumbnail

Овај документ се појављује у следећим колекцијама

Приказ основних података о документу