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dc.creatorBorojević, Ana
dc.creatorJauković, Aleksandra
dc.creatorKukolj, Tamara
dc.creatorMojsilović, Slavko
dc.creatorObradović, Hristina
dc.creatorTrivanović, Drenka
dc.creatorŽivanović, Milena
dc.creatorZečević, Željko
dc.creatorSimić, Marija
dc.creatorGobeljić, Borko
dc.creatorVujić, Dragana
dc.creatorBugarski, Diana
dc.date.accessioned2022-03-01T13:27:01Z
dc.date.available2022-03-01T13:27:01Z
dc.date.issued2022
dc.identifier.issn2218-273X
dc.identifier.urihttp://rimi.imi.bg.ac.rs/handle/123456789/1207
dc.description.abstractThe biology of vitamin D3 is well defined, as are the effects of its active metabolites on various cells, including mesenchymal stromal/stem cells (MSCs). However, the biological potential of its precursor, cholecalciferol (VD3), has not been sufficiently investigated, although its significance in regenerative medicine—mainly in combination with various biomaterial matrices—has been recognized. Given that VD3 preconditioning might also contribute to the improvement of cellular regenerative potential, the aim of this study was to investigate its effects on bone marrow (BM) MSC functions and the signaling pathways involved. For that purpose, the influence of VD3 on BM-MSCs obtained from young human donors was determined via MTT test, flow cytometric analysis, immunocytochemistry, and qRT-PCR. Our results revealed that VD3, following a 5-day treatment, stimulated proliferation, expression of pluripotency markers (NANOG, SOX2, and Oct4), and osteogenic differentiation potential in BM-MSCs, while it reduced their senescence. Moreover, increased sirtuin 1 (SIRT1) expression was detected upon treatment with VD3, which mediated VD3-promoted osteogenesis and, partially, the stemness features through NANOG and SOX2 upregulation. In contrast, the effects of VD3 on proliferation, Oct4 expression, and senescence were SIRT1-independent. Altogether, these data indicate that VD3 has strong potential to modulate BM-MSCs’ features, partially through SIRT1 signaling, although the precise mechanisms merit further investigation.
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI)
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200015/RS//
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceBiomolecules
dc.subjectbone marrow mesenchymal stromal cells (BM-MSCs)
dc.subjectvitamin D3 (cholecalciferol, VD3)
dc.subjectSIRT1
dc.subjectregenerative potential
dc.subjectstemness
dc.subjectosteogenesis
dc.titleVitamin D3 Stimulates Proliferation Capacity, Expression of Pluripotency Markers, and Osteogenesis of Human Bone Marrow Mesenchymal Stromal/Stem Cells, Partly through SIRT1 Signaling
dc.typearticle
dc.rights.licenseBY
dc.citation.issue2
dc.citation.rankM22~
dc.citation.spage323
dc.citation.volume12
dc.identifier.doi10.3390/biom12020323
dc.identifier.fulltexthttp://rimi.imi.bg.ac.rs/bitstream/id/2652/bitstream_2652.pdf
dc.type.versionpublishedVersion


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