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dc.creatorStančić, Ana
dc.creatorDrvenica, Ivana
dc.creatorIlić, Vesna
dc.creatorBugarski, Branko
dc.creatorBugarski, Diana
dc.date.accessioned2022-02-01T11:49:53Z
dc.date.available2022-02-01T11:49:53Z
dc.date.issued2022
dc.identifier.issn2227-9717
dc.identifier.urihttp://rimi.imi.bg.ac.rs/handle/123456789/1187
dc.description.abstractExploring the potential usage of the acellular preparation of porcine hemoglobin (PHb) isolated from slaughterhouse blood as a cell culture media component, we have tested its effects on the functional characteristics of stromal cells of mesodermal origin. Human peripheral blood mesenchymal stromal cells (PB-MSCs) were used in this study as a primary cell model system, along with three mouse cell lines (ATDC5, MC3T3-E1, and 3T3-L1), which represent more uniform model systems. We investigated the effect of PHb at concentrations of 0.1, 1, and 10 μM on these cells’ proliferation, cycle, and clonogenic and migratory potential, and found that PHb’s effect depended on both the cell type and its concentration. At the lowest concentration used (0.1 μM), PHb showed the least evident impact on the cell growth and migration; hence, we analyzed its effect on mesenchymal cell multilineage differentiation capacity at this concentration. Even under conditions that induce a specific type of MSC differentiation (cultivation in particular differentiation media), PHb modulated chondrogenic, osteogenic, and adipogenic differentiation, making it a potential candidate for a supplement of MSC culture. Through a model of porcine hemoglobin, these findings also contribute to improving the knowledge of extracellular hemoglobin’s influence on MSCs in vivo.
dc.publisherMDPI
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200015/RS//
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceProcesses
dc.subject3T3-L1
dc.subjectATDC5
dc.subjectdifferentiation
dc.subjectMC3T3-E1
dc.subjectPB-MSCs
dc.subjectporcine hemoglobin
dc.titleModulation of Functional Characteristics of Mesenchymal Stromal Cells by Acellular Preparation of Porcine Hemoglobin
dc.typearticle
dc.rights.licenseBY
dc.citation.issue1
dc.citation.rankM22~
dc.citation.spage32
dc.citation.volume10
dc.identifier.doi10.3390/pr10010032
dc.identifier.fulltexthttp://rimi.imi.bg.ac.rs/bitstream/id/2585/Modulation_of_Functional_Characteristics_of_Mesenchymal_Stromal_pub_2022.pdf
dc.type.versionpublishedVersion


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