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Interleukin-17 modulates uPA and MMP2 expression in human periodontal ligament mesenchymal stem cells: Involvement of the ERK1/2 MAPK pathway

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2022
Interleukin-17_modulates_uPA_and_MMP2_expression_in_human_pub_2022.pdf (1.103Mb)
Authors
Okić-Đorđević, Ivana
Kukolj, Tamara
Obradović, Hristina
Trivanović, Drenka
Petrović, Anđelija
Mojsilović, Slavko
Miletić, Maja
Bugarski, Diana
Jauković, Aleksandra
Article (Published version)
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Abstract
Periodontal disease is a chronic infection of periodontal tissue characterized by extracellular matrix (ECM) degradation due to increased expression of plasminogen activators and matrix metalloproteinases (MMPs) and various proinflammatory cytokines, including interleukin (IL)-17. Successful regeneration of damaged periodontal tissues depends on the proper functionality of periodontal ligament mesenchymal stem cells (PDLMSCs), especially the production of extracellular matrix proteases. We investigated the influence of IL-17 on ECM remodeling through modulation of urokinasetype plasminogen activator (uPA) and MMP2/MMP9 expression in human PDLMSCs at mRNA, protein and activity levels using by RT-PCR, Western blotting and zymography, respectively. Investigation of the involvement of MAPKs in these processes in PDLMSCs was determined by Western blotting, as well as by utilizing specific p38 and MEK1/2 inhibitors. Our results show that IL-17 activates MAPK signaling in PDLMSCs. Moreover, I...L-17 had no effect on MMP9 expression, but it stimulated uPA and MMP2 gene and protein expression in PDLMSCs through the activation of the ERK1/2 MAPK signaling pathway. The obtained data suggest that IL-17 contributes to ECM degradation in the periodontal ligament by stimulating uPA and MMP2 expression and activity in PDLMSCs. This information is important for understanding periodontal disease development and defining future directions of its treatment.

Keywords:
interleukin-17 / periodontal ligament mesenchymal stem cells (PDLMSC) / urokinase-type plasminogen activator (uPA) / metalloproteinase (MMP) / extracellular signal-regulated kinase (ERK)1/2 / mitogen-activated protein kinase (MAPK) signaling pathway
Source:
Archives of Biological Sciences, 2022, 74, 1, 15-24
Publisher:
  • Srpsko biološko društvo
Funding / projects:
  • Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200015 (University of Belgrade, Institute for Medical Research) (RS-200015)

DOI: 10.2298/ABS210929048O

ISSN: 0354-4664

[ Google Scholar ]
URI
http://rimi.imi.bg.ac.rs/handle/123456789/1184
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
Institut za medicinska istraživanja
TY  - JOUR
AU  - Okić-Đorđević, Ivana
AU  - Kukolj, Tamara
AU  - Obradović, Hristina
AU  - Trivanović, Drenka
AU  - Petrović, Anđelija
AU  - Mojsilović, Slavko
AU  - Miletić, Maja
AU  - Bugarski, Diana
AU  - Jauković, Aleksandra
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1184
AB  - Periodontal disease is a chronic infection of periodontal tissue characterized by extracellular matrix (ECM) degradation due to increased expression of plasminogen activators and matrix metalloproteinases (MMPs) and various proinflammatory cytokines, including interleukin (IL)-17. Successful regeneration of damaged periodontal tissues depends on the proper functionality of periodontal ligament mesenchymal stem cells (PDLMSCs), especially the production of extracellular matrix proteases. We investigated the influence of IL-17 on ECM remodeling through modulation of urokinasetype plasminogen activator (uPA) and MMP2/MMP9 expression in human PDLMSCs at mRNA, protein and activity levels using by RT-PCR, Western blotting and zymography, respectively. Investigation of the involvement of MAPKs in these processes in PDLMSCs was determined by Western blotting, as well as by utilizing specific p38 and MEK1/2 inhibitors. Our results show that IL-17 activates MAPK signaling in PDLMSCs. Moreover, IL-17 had no effect on MMP9 expression, but it stimulated uPA and MMP2 gene and protein expression in PDLMSCs through the activation of the ERK1/2 MAPK signaling pathway. The obtained data suggest that IL-17 contributes to ECM degradation in the periodontal ligament by stimulating uPA and MMP2 expression and activity in PDLMSCs. This information is important for understanding periodontal disease development and defining future directions of its treatment.
PB  - Srpsko biološko društvo
T2  - Archives of Biological Sciences
T1  - Interleukin-17 modulates uPA and MMP2 expression in human periodontal ligament mesenchymal stem cells: Involvement of the ERK1/2 MAPK pathway
EP  - 24
IS  - 1
SP  - 15
VL  - 74
DO  - 10.2298/ABS210929048O
ER  - 
@article{
author = "Okić-Đorđević, Ivana and Kukolj, Tamara and Obradović, Hristina and Trivanović, Drenka and Petrović, Anđelija and Mojsilović, Slavko and Miletić, Maja and Bugarski, Diana and Jauković, Aleksandra",
year = "2022",
abstract = "Periodontal disease is a chronic infection of periodontal tissue characterized by extracellular matrix (ECM) degradation due to increased expression of plasminogen activators and matrix metalloproteinases (MMPs) and various proinflammatory cytokines, including interleukin (IL)-17. Successful regeneration of damaged periodontal tissues depends on the proper functionality of periodontal ligament mesenchymal stem cells (PDLMSCs), especially the production of extracellular matrix proteases. We investigated the influence of IL-17 on ECM remodeling through modulation of urokinasetype plasminogen activator (uPA) and MMP2/MMP9 expression in human PDLMSCs at mRNA, protein and activity levels using by RT-PCR, Western blotting and zymography, respectively. Investigation of the involvement of MAPKs in these processes in PDLMSCs was determined by Western blotting, as well as by utilizing specific p38 and MEK1/2 inhibitors. Our results show that IL-17 activates MAPK signaling in PDLMSCs. Moreover, IL-17 had no effect on MMP9 expression, but it stimulated uPA and MMP2 gene and protein expression in PDLMSCs through the activation of the ERK1/2 MAPK signaling pathway. The obtained data suggest that IL-17 contributes to ECM degradation in the periodontal ligament by stimulating uPA and MMP2 expression and activity in PDLMSCs. This information is important for understanding periodontal disease development and defining future directions of its treatment.",
publisher = "Srpsko biološko društvo",
journal = "Archives of Biological Sciences",
title = "Interleukin-17 modulates uPA and MMP2 expression in human periodontal ligament mesenchymal stem cells: Involvement of the ERK1/2 MAPK pathway",
pages = "24-15",
number = "1",
volume = "74",
doi = "10.2298/ABS210929048O"
}
Okić-Đorđević, I., Kukolj, T., Obradović, H., Trivanović, D., Petrović, A., Mojsilović, S., Miletić, M., Bugarski, D.,& Jauković, A.. (2022). Interleukin-17 modulates uPA and MMP2 expression in human periodontal ligament mesenchymal stem cells: Involvement of the ERK1/2 MAPK pathway. in Archives of Biological Sciences
Srpsko biološko društvo., 74(1), 15-24.
https://doi.org/10.2298/ABS210929048O
Okić-Đorđević I, Kukolj T, Obradović H, Trivanović D, Petrović A, Mojsilović S, Miletić M, Bugarski D, Jauković A. Interleukin-17 modulates uPA and MMP2 expression in human periodontal ligament mesenchymal stem cells: Involvement of the ERK1/2 MAPK pathway. in Archives of Biological Sciences. 2022;74(1):15-24.
doi:10.2298/ABS210929048O .
Okić-Đorđević, Ivana, Kukolj, Tamara, Obradović, Hristina, Trivanović, Drenka, Petrović, Anđelija, Mojsilović, Slavko, Miletić, Maja, Bugarski, Diana, Jauković, Aleksandra, "Interleukin-17 modulates uPA and MMP2 expression in human periodontal ligament mesenchymal stem cells: Involvement of the ERK1/2 MAPK pathway" in Archives of Biological Sciences, 74, no. 1 (2022):15-24,
https://doi.org/10.2298/ABS210929048O . .

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