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dc.creatorJanev, Aleksandar
dc.creatorRamuta, Taja Železnik
dc.creatorTratnjek, Larisa
dc.creatorSardoč, Žiga
dc.creatorObradović, Hristina
dc.creatorMojsilović, Slavko
dc.creatorTaskovska, Milena
dc.creatorSmrkolj, Tomaž
dc.creatorKreft, Mateja Erdani
dc.date.accessioned2021-12-01T14:10:03Z
dc.date.available2021-12-01T14:10:03Z
dc.date.issued2021
dc.identifier.issn2296-4185
dc.identifier.urihttp://rimi.imi.bg.ac.rs/handle/123456789/1179
dc.description.abstractDespite being among the ten most common cancers with high recurrence rates worldwide, there have been no major breakthroughs in the standard treatment options for bladder cancer in recent years. The use of a human amniotic membrane (hAM) to treat cancer is one of the promising ideas that have emerged in recent years. This study aimed to investigate the anticancer activity of hAM homogenate on 2D and 3D cancer models. We evaluated the effects of hAM homogenates on the human muscle invasive bladder cancer urothelial (T24) cells, papillary cancer urothelial (RT4) cells and normal porcine urothelial (NPU) cells as well as on human mammary gland non-tumorigenic (MCF10a) cells and low-metastatic breast cancer (MCF7) cells. After 24 h, we observed a gradual detachment of cancerous cells from the culture surface, while the hAM homogenate did not affect the normal cells. The most pronounced effect hAM homogenate had on bladder cancer cells; however, the potency of their detachment was dependent on the treatment protocol and the preparation of hAM homogenate. We demonstrated that hAM homogenate significantly decreased the adhesion, growth, and proliferation of human bladder invasive and papillary cancer urothelial cells and did not affect normal urothelial cells even in 7-day treatment. By using light and electron microscopy we showed that hAM homogenate disrupted the architecture of 2D and 3D bladder cancer models. The information provided by our study highlights the detrimental effect of hAM homogenate on bladder cancer cells and strengthens the idea of the potential clinical application of hAM for bladder cancer treatment.
dc.publisherFrontiers Media S.A.
dc.relationSlovenian Research Agency: Young-researcher funding - project J7-2594, research core funding No. P3-0108
dc.relationMRIC UL IP-0510 Infrastructure program
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceFrontiers in Bioengineering and Biotechnology
dc.subjectcancer
dc.subjecturothelium
dc.subject2D and 3D in vitro models
dc.subjectlight and electron microscopy
dc.subjectproliferation
dc.subjectcell cycle
dc.titleDetrimental Effect of Various Preparations of the Human Amniotic Membrane Homogenate on the 2D and 3D Bladder Cancer In vitro Models
dc.typearticle
dc.rights.licenseBY
dc.citation.spage690358
dc.citation.volume9
dc.identifier.doi10.3389/fbioe.2021.690358
dc.identifier.fulltexthttp://rimi.imi.bg.ac.rs/bitstream/id/2558/Detrimental_Effect_of_Various_Preparations_pub_2021.pdf
dc.type.versionpublishedVersion


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