Exploring immunogenicity of tick salivary AV422 protein in persons exposed to ticks: prospects for utilization
Samo za registrovane korisnike
2021
Autori
Mihaljica, DarkoMarković, Dragana
Repac, Jelena
Božić, Bojan
Radulović, Željko
Veinović, Gorana
Sukara, Ratko
Ristanović, Elizabeta
Chochlakis, Dimosthenis
Nedeljković Božić, Biljana
Tomanović, Snežana
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
In order to determine whether conserved tick salivary protein AV422 is immunogenic, the goal of our study was to detect specific IgG response within at-risk populations. Study groups included 76 individuals, differing in occurrence of recently recorded tick bites and health status. Western blotting with recombinant (r) protein derived from Ixodes ricinus (Ir) was performed. IgG response to Borrelia/Rickettsia, as indicators of previous tick infestations, was also assessed. Additionally, a detailed in silico AV422 protein sequence analysis was performed, followed by modelling of the interactions between peptides and corresponding MHC II molecules by molecular docking. Anti-rIrAV422 seroprevalences among individuals exposed to ticks were high (62.5, 57.9 and 66.7%) and anti-Borrelia/Rickettsia seroprevalences were 54.2, 15.8 and 44.4% among individuals with/without recent tick bite and patients suspected of tick-borne disease, respectively. In silico analysis of AV422 protein sequence sh...owed a high level of conservation across tick genera, including also the predicted antigenic determinants specific for T and B cells. Docking to the restricted MHC II molecules was performed for all predicted AV422 T cell epitopes, and the most potent (highly immunogenic) epitope determinants were suggested. The epitope prediction reveals that tick salivary protein AV422 may elicit humoral immune response in humans, which is consistent with the high anti-rIrAV422 seroprevalence in tested at-risk subjects. Tick-borne diseases are a growing public health concern worldwide, and AV422 is potentially useful in clinical practice and epidemiological studies.
Ključne reči:
AV422 / Humoral immune response / Immunoinformatics / Tick saliva proteinIzvor:
Experimental and Applied Acarology, 2021, 85, 1, 83-99Izdavač:
- Springer
Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200015 (Univerzitet u Beogradu, Institut za medicinska istraživanja) (RS-200015)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200178 (Univerzitet u Beogradu, Biološki fakultet) (RS-200178)
Institucija/grupa
Institut za medicinska istraživanjaTY - JOUR AU - Mihaljica, Darko AU - Marković, Dragana AU - Repac, Jelena AU - Božić, Bojan AU - Radulović, Željko AU - Veinović, Gorana AU - Sukara, Ratko AU - Ristanović, Elizabeta AU - Chochlakis, Dimosthenis AU - Nedeljković Božić, Biljana AU - Tomanović, Snežana PY - 2021 UR - http://rimi.imi.bg.ac.rs/handle/123456789/1160 AB - In order to determine whether conserved tick salivary protein AV422 is immunogenic, the goal of our study was to detect specific IgG response within at-risk populations. Study groups included 76 individuals, differing in occurrence of recently recorded tick bites and health status. Western blotting with recombinant (r) protein derived from Ixodes ricinus (Ir) was performed. IgG response to Borrelia/Rickettsia, as indicators of previous tick infestations, was also assessed. Additionally, a detailed in silico AV422 protein sequence analysis was performed, followed by modelling of the interactions between peptides and corresponding MHC II molecules by molecular docking. Anti-rIrAV422 seroprevalences among individuals exposed to ticks were high (62.5, 57.9 and 66.7%) and anti-Borrelia/Rickettsia seroprevalences were 54.2, 15.8 and 44.4% among individuals with/without recent tick bite and patients suspected of tick-borne disease, respectively. In silico analysis of AV422 protein sequence showed a high level of conservation across tick genera, including also the predicted antigenic determinants specific for T and B cells. Docking to the restricted MHC II molecules was performed for all predicted AV422 T cell epitopes, and the most potent (highly immunogenic) epitope determinants were suggested. The epitope prediction reveals that tick salivary protein AV422 may elicit humoral immune response in humans, which is consistent with the high anti-rIrAV422 seroprevalence in tested at-risk subjects. Tick-borne diseases are a growing public health concern worldwide, and AV422 is potentially useful in clinical practice and epidemiological studies. PB - Springer T2 - Experimental and Applied Acarology T1 - Exploring immunogenicity of tick salivary AV422 protein in persons exposed to ticks: prospects for utilization EP - 99 IS - 1 SP - 83 VL - 85 DO - 10.1007/s10493-021-00653-z ER -
@article{ author = "Mihaljica, Darko and Marković, Dragana and Repac, Jelena and Božić, Bojan and Radulović, Željko and Veinović, Gorana and Sukara, Ratko and Ristanović, Elizabeta and Chochlakis, Dimosthenis and Nedeljković Božić, Biljana and Tomanović, Snežana", year = "2021", abstract = "In order to determine whether conserved tick salivary protein AV422 is immunogenic, the goal of our study was to detect specific IgG response within at-risk populations. Study groups included 76 individuals, differing in occurrence of recently recorded tick bites and health status. Western blotting with recombinant (r) protein derived from Ixodes ricinus (Ir) was performed. IgG response to Borrelia/Rickettsia, as indicators of previous tick infestations, was also assessed. Additionally, a detailed in silico AV422 protein sequence analysis was performed, followed by modelling of the interactions between peptides and corresponding MHC II molecules by molecular docking. Anti-rIrAV422 seroprevalences among individuals exposed to ticks were high (62.5, 57.9 and 66.7%) and anti-Borrelia/Rickettsia seroprevalences were 54.2, 15.8 and 44.4% among individuals with/without recent tick bite and patients suspected of tick-borne disease, respectively. In silico analysis of AV422 protein sequence showed a high level of conservation across tick genera, including also the predicted antigenic determinants specific for T and B cells. Docking to the restricted MHC II molecules was performed for all predicted AV422 T cell epitopes, and the most potent (highly immunogenic) epitope determinants were suggested. The epitope prediction reveals that tick salivary protein AV422 may elicit humoral immune response in humans, which is consistent with the high anti-rIrAV422 seroprevalence in tested at-risk subjects. Tick-borne diseases are a growing public health concern worldwide, and AV422 is potentially useful in clinical practice and epidemiological studies.", publisher = "Springer", journal = "Experimental and Applied Acarology", title = "Exploring immunogenicity of tick salivary AV422 protein in persons exposed to ticks: prospects for utilization", pages = "99-83", number = "1", volume = "85", doi = "10.1007/s10493-021-00653-z" }
Mihaljica, D., Marković, D., Repac, J., Božić, B., Radulović, Ž., Veinović, G., Sukara, R., Ristanović, E., Chochlakis, D., Nedeljković Božić, B.,& Tomanović, S.. (2021). Exploring immunogenicity of tick salivary AV422 protein in persons exposed to ticks: prospects for utilization. in Experimental and Applied Acarology Springer., 85(1), 83-99. https://doi.org/10.1007/s10493-021-00653-z
Mihaljica D, Marković D, Repac J, Božić B, Radulović Ž, Veinović G, Sukara R, Ristanović E, Chochlakis D, Nedeljković Božić B, Tomanović S. Exploring immunogenicity of tick salivary AV422 protein in persons exposed to ticks: prospects for utilization. in Experimental and Applied Acarology. 2021;85(1):83-99. doi:10.1007/s10493-021-00653-z .
Mihaljica, Darko, Marković, Dragana, Repac, Jelena, Božić, Bojan, Radulović, Željko, Veinović, Gorana, Sukara, Ratko, Ristanović, Elizabeta, Chochlakis, Dimosthenis, Nedeljković Božić, Biljana , Tomanović, Snežana, "Exploring immunogenicity of tick salivary AV422 protein in persons exposed to ticks: prospects for utilization" in Experimental and Applied Acarology, 85, no. 1 (2021):83-99, https://doi.org/10.1007/s10493-021-00653-z . .