Immunohistochemical Analysis of 4-HNE, NGAL, and HO-1 Tissue Expression after Apocynin Treatment and HBO Preconditioning in Postischemic Acute Kidney Injury Induced in Spontaneously Hypertensive Rats
Authors
Kovačević, Sanjin
Ivanov, Milan

Životić, Maja

Brkić, Predrag
Miloradović, Zoran

Jeremić, Rada

Mihailović-Stanojević, Nevena

Vajić, Una-Jovana

Karanović, Danijela

Jovović, Đurđica

Nešović Ostojić, Jelena
Article (Published version)
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Oxidative stress has been considered as a central aggravating factor in the development of postischemic acute kidney injury (AKI). The aim of this study was to perform the immunohistochemical analysis of 4-hydroxynonenal (4-HNE), neutrophil gelatinase-associated lipocalin (NGAL), and heme oxygenase-1 (HO-1) tissue expression after apocynin (APO) treatment and hyperbaric oxygenation (HBO) preconditioning, applied as single or combined protocol, in postischemic acute kidney injury induced in spontaneously hypertensive rats (SHR). Twenty-four hours before AKI induction, HBO preconditioning was carried out by exposing to pure oxygen (2.026 bar) twice a day, for 60 min in two consecutive days. Acute kidney injury was induced by removal of the right kidney while the left renal artery was occluded for 45 min by atraumatic clamp. Apocynin was applied in a dose of 40 mg/kg body weight, intravenously, 5 min before reperfusion. We showed increased 4-HNE renal expression in postischemic AKI compar...ed to Sham-operated (SHAM) group. Apocynin treatment, with or without HBO preconditioning, improved creatinine and phosphate clearances, in postischemic AKI. This improvement in renal function was accompanied with decreased 4-HNE, while HO-1 kidney expression restored close to the control group level. NGAL renal expression was also decreased after apocynin treatment, and HBO preconditioning, with or without APO treatment. Considering our results, we can say that 4-HNE tissue expression can be used as a marker of oxidative stress in postischemic AKI. On the other hand, apocynin treatment and HBO preconditioning reduced oxidative damage, and this protective effect might be expected even in experimental hypertensive condition.
Keywords:
4-hydroxynonenal / acute kidney injury / apocynin / heme oxygenase-1 / hyperbaric oxygen preconditioning / neutrophil gelatinase-associated lipocalin / oxidative stressSource:
Antioxidants, 2021, 10, 8, 1163-Publisher:
- Multidisciplinary Digital Publishing Institute (MDPI)
Funding / projects:
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200015 (University of Belgrade, Institute for Medical Research) (RS-200015)
- Characterisation of human renal stem/progenitor cells: identification of new cell surface markers of renal multipotent cells which could have regenerative role in kidney lesions (RS-175047)
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Institut za medicinska istraživanjaTY - JOUR AU - Kovačević, Sanjin AU - Ivanov, Milan AU - Životić, Maja AU - Brkić, Predrag AU - Miloradović, Zoran AU - Jeremić, Rada AU - Mihailović-Stanojević, Nevena AU - Vajić, Una-Jovana AU - Karanović, Danijela AU - Jovović, Đurđica AU - Nešović Ostojić, Jelena PY - 2021 UR - http://rimi.imi.bg.ac.rs/handle/123456789/1155 AB - Oxidative stress has been considered as a central aggravating factor in the development of postischemic acute kidney injury (AKI). The aim of this study was to perform the immunohistochemical analysis of 4-hydroxynonenal (4-HNE), neutrophil gelatinase-associated lipocalin (NGAL), and heme oxygenase-1 (HO-1) tissue expression after apocynin (APO) treatment and hyperbaric oxygenation (HBO) preconditioning, applied as single or combined protocol, in postischemic acute kidney injury induced in spontaneously hypertensive rats (SHR). Twenty-four hours before AKI induction, HBO preconditioning was carried out by exposing to pure oxygen (2.026 bar) twice a day, for 60 min in two consecutive days. Acute kidney injury was induced by removal of the right kidney while the left renal artery was occluded for 45 min by atraumatic clamp. Apocynin was applied in a dose of 40 mg/kg body weight, intravenously, 5 min before reperfusion. We showed increased 4-HNE renal expression in postischemic AKI compared to Sham-operated (SHAM) group. Apocynin treatment, with or without HBO preconditioning, improved creatinine and phosphate clearances, in postischemic AKI. This improvement in renal function was accompanied with decreased 4-HNE, while HO-1 kidney expression restored close to the control group level. NGAL renal expression was also decreased after apocynin treatment, and HBO preconditioning, with or without APO treatment. Considering our results, we can say that 4-HNE tissue expression can be used as a marker of oxidative stress in postischemic AKI. On the other hand, apocynin treatment and HBO preconditioning reduced oxidative damage, and this protective effect might be expected even in experimental hypertensive condition. PB - Multidisciplinary Digital Publishing Institute (MDPI) T2 - Antioxidants T1 - Immunohistochemical Analysis of 4-HNE, NGAL, and HO-1 Tissue Expression after Apocynin Treatment and HBO Preconditioning in Postischemic Acute Kidney Injury Induced in Spontaneously Hypertensive Rats IS - 8 SP - 1163 VL - 10 DO - 10.3390/antiox10081163 ER -
@article{ author = "Kovačević, Sanjin and Ivanov, Milan and Životić, Maja and Brkić, Predrag and Miloradović, Zoran and Jeremić, Rada and Mihailović-Stanojević, Nevena and Vajić, Una-Jovana and Karanović, Danijela and Jovović, Đurđica and Nešović Ostojić, Jelena", year = "2021", abstract = "Oxidative stress has been considered as a central aggravating factor in the development of postischemic acute kidney injury (AKI). The aim of this study was to perform the immunohistochemical analysis of 4-hydroxynonenal (4-HNE), neutrophil gelatinase-associated lipocalin (NGAL), and heme oxygenase-1 (HO-1) tissue expression after apocynin (APO) treatment and hyperbaric oxygenation (HBO) preconditioning, applied as single or combined protocol, in postischemic acute kidney injury induced in spontaneously hypertensive rats (SHR). Twenty-four hours before AKI induction, HBO preconditioning was carried out by exposing to pure oxygen (2.026 bar) twice a day, for 60 min in two consecutive days. Acute kidney injury was induced by removal of the right kidney while the left renal artery was occluded for 45 min by atraumatic clamp. Apocynin was applied in a dose of 40 mg/kg body weight, intravenously, 5 min before reperfusion. We showed increased 4-HNE renal expression in postischemic AKI compared to Sham-operated (SHAM) group. Apocynin treatment, with or without HBO preconditioning, improved creatinine and phosphate clearances, in postischemic AKI. This improvement in renal function was accompanied with decreased 4-HNE, while HO-1 kidney expression restored close to the control group level. NGAL renal expression was also decreased after apocynin treatment, and HBO preconditioning, with or without APO treatment. Considering our results, we can say that 4-HNE tissue expression can be used as a marker of oxidative stress in postischemic AKI. On the other hand, apocynin treatment and HBO preconditioning reduced oxidative damage, and this protective effect might be expected even in experimental hypertensive condition.", publisher = "Multidisciplinary Digital Publishing Institute (MDPI)", journal = "Antioxidants", title = "Immunohistochemical Analysis of 4-HNE, NGAL, and HO-1 Tissue Expression after Apocynin Treatment and HBO Preconditioning in Postischemic Acute Kidney Injury Induced in Spontaneously Hypertensive Rats", number = "8", pages = "1163", volume = "10", doi = "10.3390/antiox10081163" }
Kovačević, S., Ivanov, M., Životić, M., Brkić, P., Miloradović, Z., Jeremić, R., Mihailović-Stanojević, N., Vajić, U., Karanović, D., Jovović, Đ.,& Nešović Ostojić, J.. (2021). Immunohistochemical Analysis of 4-HNE, NGAL, and HO-1 Tissue Expression after Apocynin Treatment and HBO Preconditioning in Postischemic Acute Kidney Injury Induced in Spontaneously Hypertensive Rats. in Antioxidants Multidisciplinary Digital Publishing Institute (MDPI)., 10(8), 1163. https://doi.org/10.3390/antiox10081163
Kovačević S, Ivanov M, Životić M, Brkić P, Miloradović Z, Jeremić R, Mihailović-Stanojević N, Vajić U, Karanović D, Jovović Đ, Nešović Ostojić J. Immunohistochemical Analysis of 4-HNE, NGAL, and HO-1 Tissue Expression after Apocynin Treatment and HBO Preconditioning in Postischemic Acute Kidney Injury Induced in Spontaneously Hypertensive Rats. in Antioxidants. 2021;10(8):1163. doi:10.3390/antiox10081163 .
Kovačević, Sanjin, Ivanov, Milan, Životić, Maja, Brkić, Predrag, Miloradović, Zoran, Jeremić, Rada, Mihailović-Stanojević, Nevena, Vajić, Una-Jovana, Karanović, Danijela, Jovović, Đurđica, Nešović Ostojić, Jelena, "Immunohistochemical Analysis of 4-HNE, NGAL, and HO-1 Tissue Expression after Apocynin Treatment and HBO Preconditioning in Postischemic Acute Kidney Injury Induced in Spontaneously Hypertensive Rats" in Antioxidants, 10, no. 8 (2021):1163, https://doi.org/10.3390/antiox10081163 . .