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Immunohistochemical Analysis of 4-HNE, NGAL, and HO-1 Tissue Expression after Apocynin Treatment and HBO Preconditioning in Postischemic Acute Kidney Injury Induced in Spontaneously Hypertensive Rats

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Authors
Kovačević, Sanjin
Ivanov, Milan
Životić, Maja
Brkić, Predrag
Miloradović, Zoran
Jeremić, Rada
Mihailović-Stanojević, Nevena
Vajić, Una-Jovana
Karanović, Danijela
Jovović, Đurđica
Nešović Ostojić, Jelena
Article (Published version)
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Abstract
Oxidative stress has been considered as a central aggravating factor in the development of postischemic acute kidney injury (AKI). The aim of this study was to perform the immunohistochemical analysis of 4-hydroxynonenal (4-HNE), neutrophil gelatinase-associated lipocalin (NGAL), and heme oxygenase-1 (HO-1) tissue expression after apocynin (APO) treatment and hyperbaric oxygenation (HBO) preconditioning, applied as single or combined protocol, in postischemic acute kidney injury induced in spontaneously hypertensive rats (SHR). Twenty-four hours before AKI induction, HBO preconditioning was carried out by exposing to pure oxygen (2.026 bar) twice a day, for 60 min in two consecutive days. Acute kidney injury was induced by removal of the right kidney while the left renal artery was occluded for 45 min by atraumatic clamp. Apocynin was applied in a dose of 40 mg/kg body weight, intravenously, 5 min before reperfusion. We showed increased 4-HNE renal expression in postischemic AKI compar...ed to Sham-operated (SHAM) group. Apocynin treatment, with or without HBO preconditioning, improved creatinine and phosphate clearances, in postischemic AKI. This improvement in renal function was accompanied with decreased 4-HNE, while HO-1 kidney expression restored close to the control group level. NGAL renal expression was also decreased after apocynin treatment, and HBO preconditioning, with or without APO treatment. Considering our results, we can say that 4-HNE tissue expression can be used as a marker of oxidative stress in postischemic AKI. On the other hand, apocynin treatment and HBO preconditioning reduced oxidative damage, and this protective effect might be expected even in experimental hypertensive condition.

Keywords:
4-hydroxynonenal / acute kidney injury / apocynin / heme oxygenase-1 / hyperbaric oxygen preconditioning / neutrophil gelatinase-associated lipocalin / oxidative stress
Source:
Antioxidants, 2021, 10, 8, 1163-
Publisher:
  • Multidisciplinary Digital Publishing Institute (MDPI)
Funding / projects:
  • Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200015 (University of Belgrade, Institute for Medical Research) (RS-200015)
  • Characterisation of human renal stem/progenitor cells: identification of new cell surface markers of renal multipotent cells which could have regenerative role in kidney lesions (RS-175047)

DOI: 10.3390/antiox10081163

ISSN: 2076-3921

[ Google Scholar ]
URI
http://rimi.imi.bg.ac.rs/handle/123456789/1155
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
Institut za medicinska istraživanja
TY  - JOUR
AU  - Kovačević, Sanjin
AU  - Ivanov, Milan
AU  - Životić, Maja
AU  - Brkić, Predrag
AU  - Miloradović, Zoran
AU  - Jeremić, Rada
AU  - Mihailović-Stanojević, Nevena
AU  - Vajić, Una-Jovana
AU  - Karanović, Danijela
AU  - Jovović, Đurđica
AU  - Nešović Ostojić, Jelena
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1155
AB  - Oxidative stress has been considered as a central aggravating factor in the development of postischemic acute kidney injury (AKI). The aim of this study was to perform the immunohistochemical analysis of 4-hydroxynonenal (4-HNE), neutrophil gelatinase-associated lipocalin (NGAL), and heme oxygenase-1 (HO-1) tissue expression after apocynin (APO) treatment and hyperbaric oxygenation (HBO) preconditioning, applied as single or combined protocol, in postischemic acute kidney injury induced in spontaneously hypertensive rats (SHR). Twenty-four hours before AKI induction, HBO preconditioning was carried out by exposing to pure oxygen (2.026 bar) twice a day, for 60 min in two consecutive days. Acute kidney injury was induced by removal of the right kidney while the left renal artery was occluded for 45 min by atraumatic clamp. Apocynin was applied in a dose of 40 mg/kg body weight, intravenously, 5 min before reperfusion. We showed increased 4-HNE renal expression in postischemic AKI compared to Sham-operated (SHAM) group. Apocynin treatment, with or without HBO preconditioning, improved creatinine and phosphate clearances, in postischemic AKI. This improvement in renal function was accompanied with decreased 4-HNE, while HO-1 kidney expression restored close to the control group level. NGAL renal expression was also decreased after apocynin treatment, and HBO preconditioning, with or without APO treatment. Considering our results, we can say that 4-HNE tissue expression can be used as a marker of oxidative stress in postischemic AKI. On the other hand, apocynin treatment and HBO preconditioning reduced oxidative damage, and this protective effect might be expected even in experimental hypertensive condition.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Antioxidants
T1  - Immunohistochemical Analysis of 4-HNE, NGAL, and HO-1 Tissue Expression after Apocynin Treatment and HBO Preconditioning in Postischemic Acute Kidney Injury Induced in Spontaneously Hypertensive Rats
IS  - 8
SP  - 1163
VL  - 10
DO  - 10.3390/antiox10081163
ER  - 
@article{
author = "Kovačević, Sanjin and Ivanov, Milan and Životić, Maja and Brkić, Predrag and Miloradović, Zoran and Jeremić, Rada and Mihailović-Stanojević, Nevena and Vajić, Una-Jovana and Karanović, Danijela and Jovović, Đurđica and Nešović Ostojić, Jelena",
year = "2021",
abstract = "Oxidative stress has been considered as a central aggravating factor in the development of postischemic acute kidney injury (AKI). The aim of this study was to perform the immunohistochemical analysis of 4-hydroxynonenal (4-HNE), neutrophil gelatinase-associated lipocalin (NGAL), and heme oxygenase-1 (HO-1) tissue expression after apocynin (APO) treatment and hyperbaric oxygenation (HBO) preconditioning, applied as single or combined protocol, in postischemic acute kidney injury induced in spontaneously hypertensive rats (SHR). Twenty-four hours before AKI induction, HBO preconditioning was carried out by exposing to pure oxygen (2.026 bar) twice a day, for 60 min in two consecutive days. Acute kidney injury was induced by removal of the right kidney while the left renal artery was occluded for 45 min by atraumatic clamp. Apocynin was applied in a dose of 40 mg/kg body weight, intravenously, 5 min before reperfusion. We showed increased 4-HNE renal expression in postischemic AKI compared to Sham-operated (SHAM) group. Apocynin treatment, with or without HBO preconditioning, improved creatinine and phosphate clearances, in postischemic AKI. This improvement in renal function was accompanied with decreased 4-HNE, while HO-1 kidney expression restored close to the control group level. NGAL renal expression was also decreased after apocynin treatment, and HBO preconditioning, with or without APO treatment. Considering our results, we can say that 4-HNE tissue expression can be used as a marker of oxidative stress in postischemic AKI. On the other hand, apocynin treatment and HBO preconditioning reduced oxidative damage, and this protective effect might be expected even in experimental hypertensive condition.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Antioxidants",
title = "Immunohistochemical Analysis of 4-HNE, NGAL, and HO-1 Tissue Expression after Apocynin Treatment and HBO Preconditioning in Postischemic Acute Kidney Injury Induced in Spontaneously Hypertensive Rats",
number = "8",
pages = "1163",
volume = "10",
doi = "10.3390/antiox10081163"
}
Kovačević, S., Ivanov, M., Životić, M., Brkić, P., Miloradović, Z., Jeremić, R., Mihailović-Stanojević, N., Vajić, U., Karanović, D., Jovović, Đ.,& Nešović Ostojić, J.. (2021). Immunohistochemical Analysis of 4-HNE, NGAL, and HO-1 Tissue Expression after Apocynin Treatment and HBO Preconditioning in Postischemic Acute Kidney Injury Induced in Spontaneously Hypertensive Rats. in Antioxidants
Multidisciplinary Digital Publishing Institute (MDPI)., 10(8), 1163.
https://doi.org/10.3390/antiox10081163
Kovačević S, Ivanov M, Životić M, Brkić P, Miloradović Z, Jeremić R, Mihailović-Stanojević N, Vajić U, Karanović D, Jovović Đ, Nešović Ostojić J. Immunohistochemical Analysis of 4-HNE, NGAL, and HO-1 Tissue Expression after Apocynin Treatment and HBO Preconditioning in Postischemic Acute Kidney Injury Induced in Spontaneously Hypertensive Rats. in Antioxidants. 2021;10(8):1163.
doi:10.3390/antiox10081163 .
Kovačević, Sanjin, Ivanov, Milan, Životić, Maja, Brkić, Predrag, Miloradović, Zoran, Jeremić, Rada, Mihailović-Stanojević, Nevena, Vajić, Una-Jovana, Karanović, Danijela, Jovović, Đurđica, Nešović Ostojić, Jelena, "Immunohistochemical Analysis of 4-HNE, NGAL, and HO-1 Tissue Expression after Apocynin Treatment and HBO Preconditioning in Postischemic Acute Kidney Injury Induced in Spontaneously Hypertensive Rats" in Antioxidants, 10, no. 8 (2021):1163,
https://doi.org/10.3390/antiox10081163 . .

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