Modulating stemness of mesenchymal stem cells from exfoliated deciduous and permanent teeth by IL-17 and bFGF
Authorized Users Only
2021
Authors
Jauković, AleksandraKukolj, Tamara
Trivanović, Drenka
Okić Đorđević, Ivana
Obradović, Hristina
Miletić, Maja
Petrović, Vanja
Mojsilović, Slavko
Bugarski, Diana
Article (Published version)
Metadata
Show full item recordAbstract
Mesenchymal stem cells (MSCs) have been identified within dental pulp tissues of exfoliated deciduous (SHEDs) and permanent (DPSCs) teeth. Although differences in their proliferative and differentiation properties were revealed, variability in SHEDs and DPSCs responsiveness to growth factors and cytokines have not been studied before. Here, we investigated the influence of interleukin-17 (IL-17) and basic fibroblast growth factor (bFGF) on stemness features of SHEDs and DPSCs by analyzing their proliferation, clonogenicity, cell cycle progression, pluripotency markers expression and differentiation after 7-day treatment. Results indicated that IL-17 and bFGF differently affected SHEDs and DPSCs proliferation and clonogenicity, since bFGF increased proliferative and clonogenic potential of both cell types, while IL-17 similarly affected SHEDs, exerting no effects on adult counterparts DPSCs. In addition, both factors stimulated NANOG, OCT4, and SOX2 pluripotency markers expression in SH...EDs and DPSCs showing diverse intracellular expression patterns dependent on MSCs type. As for the differentiation capacity, both factors displayed comparable effects on SHEDs and DPSCs, including stimulatory effect of IL-17 on early osteogenesis in contrast to the strong inhibitory effect showed for bFGF, while having no impact on SHEDs and DPSCs chondrogenesis. Moreover, bFGF combined with IL-17 reduced CD90 and stimulated CD73 expression on both types of MSCs, whereas each factor induced IL-6 expression indicating its' role in IL-17/bFGF-modulated properties of SHEDs and DPSCs. All these data demonstrated that dental pulp MSCs from primary and permanent teeth exert intrinsic features, providing novel evidence on how IL-17 and bFGF affect stem cell properties important for regeneration of dental pulp at different ages.
Keywords:
dental pulp / DPSC / FGF / IL-17 / mesenchymal stem cells / SHED / stemnessSource:
Journal of Cellular Physiology, 2021, 236, 11, 7322-7341Publisher:
- Wiley-Blackwell
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200015 (University of Belgrade, Institute for Medical Research) (RS-MESTD-inst-2020-200015)
Collections
Institution/Community
Institut za medicinska istraživanjaTY - JOUR AU - Jauković, Aleksandra AU - Kukolj, Tamara AU - Trivanović, Drenka AU - Okić Đorđević, Ivana AU - Obradović, Hristina AU - Miletić, Maja AU - Petrović, Vanja AU - Mojsilović, Slavko AU - Bugarski, Diana PY - 2021 UR - http://rimi.imi.bg.ac.rs/handle/123456789/1149 AB - Mesenchymal stem cells (MSCs) have been identified within dental pulp tissues of exfoliated deciduous (SHEDs) and permanent (DPSCs) teeth. Although differences in their proliferative and differentiation properties were revealed, variability in SHEDs and DPSCs responsiveness to growth factors and cytokines have not been studied before. Here, we investigated the influence of interleukin-17 (IL-17) and basic fibroblast growth factor (bFGF) on stemness features of SHEDs and DPSCs by analyzing their proliferation, clonogenicity, cell cycle progression, pluripotency markers expression and differentiation after 7-day treatment. Results indicated that IL-17 and bFGF differently affected SHEDs and DPSCs proliferation and clonogenicity, since bFGF increased proliferative and clonogenic potential of both cell types, while IL-17 similarly affected SHEDs, exerting no effects on adult counterparts DPSCs. In addition, both factors stimulated NANOG, OCT4, and SOX2 pluripotency markers expression in SHEDs and DPSCs showing diverse intracellular expression patterns dependent on MSCs type. As for the differentiation capacity, both factors displayed comparable effects on SHEDs and DPSCs, including stimulatory effect of IL-17 on early osteogenesis in contrast to the strong inhibitory effect showed for bFGF, while having no impact on SHEDs and DPSCs chondrogenesis. Moreover, bFGF combined with IL-17 reduced CD90 and stimulated CD73 expression on both types of MSCs, whereas each factor induced IL-6 expression indicating its' role in IL-17/bFGF-modulated properties of SHEDs and DPSCs. All these data demonstrated that dental pulp MSCs from primary and permanent teeth exert intrinsic features, providing novel evidence on how IL-17 and bFGF affect stem cell properties important for regeneration of dental pulp at different ages. PB - Wiley-Blackwell T2 - Journal of Cellular Physiology T1 - Modulating stemness of mesenchymal stem cells from exfoliated deciduous and permanent teeth by IL-17 and bFGF EP - 7341 IS - 11 SP - 7322 VL - 236 DO - 10.1002/jcp.30399 ER -
@article{ author = "Jauković, Aleksandra and Kukolj, Tamara and Trivanović, Drenka and Okić Đorđević, Ivana and Obradović, Hristina and Miletić, Maja and Petrović, Vanja and Mojsilović, Slavko and Bugarski, Diana", year = "2021", abstract = "Mesenchymal stem cells (MSCs) have been identified within dental pulp tissues of exfoliated deciduous (SHEDs) and permanent (DPSCs) teeth. Although differences in their proliferative and differentiation properties were revealed, variability in SHEDs and DPSCs responsiveness to growth factors and cytokines have not been studied before. Here, we investigated the influence of interleukin-17 (IL-17) and basic fibroblast growth factor (bFGF) on stemness features of SHEDs and DPSCs by analyzing their proliferation, clonogenicity, cell cycle progression, pluripotency markers expression and differentiation after 7-day treatment. Results indicated that IL-17 and bFGF differently affected SHEDs and DPSCs proliferation and clonogenicity, since bFGF increased proliferative and clonogenic potential of both cell types, while IL-17 similarly affected SHEDs, exerting no effects on adult counterparts DPSCs. In addition, both factors stimulated NANOG, OCT4, and SOX2 pluripotency markers expression in SHEDs and DPSCs showing diverse intracellular expression patterns dependent on MSCs type. As for the differentiation capacity, both factors displayed comparable effects on SHEDs and DPSCs, including stimulatory effect of IL-17 on early osteogenesis in contrast to the strong inhibitory effect showed for bFGF, while having no impact on SHEDs and DPSCs chondrogenesis. Moreover, bFGF combined with IL-17 reduced CD90 and stimulated CD73 expression on both types of MSCs, whereas each factor induced IL-6 expression indicating its' role in IL-17/bFGF-modulated properties of SHEDs and DPSCs. All these data demonstrated that dental pulp MSCs from primary and permanent teeth exert intrinsic features, providing novel evidence on how IL-17 and bFGF affect stem cell properties important for regeneration of dental pulp at different ages.", publisher = "Wiley-Blackwell", journal = "Journal of Cellular Physiology", title = "Modulating stemness of mesenchymal stem cells from exfoliated deciduous and permanent teeth by IL-17 and bFGF", pages = "7341-7322", number = "11", volume = "236", doi = "10.1002/jcp.30399" }
Jauković, A., Kukolj, T., Trivanović, D., Okić Đorđević, I., Obradović, H., Miletić, M., Petrović, V., Mojsilović, S.,& Bugarski, D.. (2021). Modulating stemness of mesenchymal stem cells from exfoliated deciduous and permanent teeth by IL-17 and bFGF. in Journal of Cellular Physiology Wiley-Blackwell., 236(11), 7322-7341. https://doi.org/10.1002/jcp.30399
Jauković A, Kukolj T, Trivanović D, Okić Đorđević I, Obradović H, Miletić M, Petrović V, Mojsilović S, Bugarski D. Modulating stemness of mesenchymal stem cells from exfoliated deciduous and permanent teeth by IL-17 and bFGF. in Journal of Cellular Physiology. 2021;236(11):7322-7341. doi:10.1002/jcp.30399 .
Jauković, Aleksandra, Kukolj, Tamara, Trivanović, Drenka, Okić Đorđević, Ivana, Obradović, Hristina, Miletić, Maja, Petrović, Vanja, Mojsilović, Slavko, Bugarski, Diana, "Modulating stemness of mesenchymal stem cells from exfoliated deciduous and permanent teeth by IL-17 and bFGF" in Journal of Cellular Physiology, 236, no. 11 (2021):7322-7341, https://doi.org/10.1002/jcp.30399 . .