The effects of a new material based on hydroxyapatite and calcium silicates, named ALBO-MPCA, were investigated on the liver, kidney and spleen. The material was administrated orally for 120 days in an in vivo model in Wistar rats, and untreated animals served as a control. Hematological and biochemical blood parameters were analyzed. Qualitative histological analysis of tissues, change in mitotic activity of cells, and histological characteristics was conducted, as well as quantitative stereological analysis of parenchymal cells, blood sinusoids, and connective tissues. Additionally, the protein expressions of Ki67 and CD68 markers were evaluated. Histological analysis revealed no pathological changes after the tested period. It showed the preservation of the architecture of blood sinusoids and epithelial cells and the presence of mitosis. Additionally, the significantly increased number of the Ki67 in the presence of ALBO-MPCA confirmed the proliferative effect of the material notice...d by stereological analysis, while immunoreactive CD68 positive cells did not differ between groups. The study showed non-toxicity of the tested material based on the effects on the hematological, biochemical, and observed histological parameters; in addition, it showed evidence of its biocompatibility. These results could be the basis for further steps toward the application of tested materials in endodontics.
TY - JOUR
AU - Paraš, Smiljana
AU - Trišić, Dijana
AU - Mitrović-Ajtić, Olivera
AU - Antonijević, Đorđe
AU - Čolović, Božana
AU - Drobne, Damjana
AU - Jokanović, Vukoman
PY - 2021
UR - http://rimi.imi.bg.ac.rs/handle/123456789/1134
AB - The effects of a new material based on hydroxyapatite and calcium silicates, named ALBO-MPCA, were investigated on the liver, kidney and spleen. The material was administrated orally for 120 days in an in vivo model in Wistar rats, and untreated animals served as a control. Hematological and biochemical blood parameters were analyzed. Qualitative histological analysis of tissues, change in mitotic activity of cells, and histological characteristics was conducted, as well as quantitative stereological analysis of parenchymal cells, blood sinusoids, and connective tissues. Additionally, the protein expressions of Ki67 and CD68 markers were evaluated. Histological analysis revealed no pathological changes after the tested period. It showed the preservation of the architecture of blood sinusoids and epithelial cells and the presence of mitosis. Additionally, the significantly increased number of the Ki67 in the presence of ALBO-MPCA confirmed the proliferative effect of the material noticed by stereological analysis, while immunoreactive CD68 positive cells did not differ between groups. The study showed non-toxicity of the tested material based on the effects on the hematological, biochemical, and observed histological parameters; in addition, it showed evidence of its biocompatibility. These results could be the basis for further steps toward the application of tested materials in endodontics.
PB - MDPI
T2 - International Journal of Molecular Science
T1 - Biocompatibility Study of a New Dental Cement Based on Hydroxyapatite and Calcium Silicates: Focus on Liver, Kidney and Spleen Tissue Effects
IS - 11
SP - 5468
VL - 22
DO - 10.3390/ijms22115468
ER -
@article{
author = "Paraš, Smiljana and Trišić, Dijana and Mitrović-Ajtić, Olivera and Antonijević, Đorđe and Čolović, Božana and Drobne, Damjana and Jokanović, Vukoman",
year = "2021",
abstract = "The effects of a new material based on hydroxyapatite and calcium silicates, named ALBO-MPCA, were investigated on the liver, kidney and spleen. The material was administrated orally for 120 days in an in vivo model in Wistar rats, and untreated animals served as a control. Hematological and biochemical blood parameters were analyzed. Qualitative histological analysis of tissues, change in mitotic activity of cells, and histological characteristics was conducted, as well as quantitative stereological analysis of parenchymal cells, blood sinusoids, and connective tissues. Additionally, the protein expressions of Ki67 and CD68 markers were evaluated. Histological analysis revealed no pathological changes after the tested period. It showed the preservation of the architecture of blood sinusoids and epithelial cells and the presence of mitosis. Additionally, the significantly increased number of the Ki67 in the presence of ALBO-MPCA confirmed the proliferative effect of the material noticed by stereological analysis, while immunoreactive CD68 positive cells did not differ between groups. The study showed non-toxicity of the tested material based on the effects on the hematological, biochemical, and observed histological parameters; in addition, it showed evidence of its biocompatibility. These results could be the basis for further steps toward the application of tested materials in endodontics.",
publisher = "MDPI",
journal = "International Journal of Molecular Science",
title = "Biocompatibility Study of a New Dental Cement Based on Hydroxyapatite and Calcium Silicates: Focus on Liver, Kidney and Spleen Tissue Effects",
number = "11",
pages = "5468",
volume = "22",
doi = "10.3390/ijms22115468"
}
Paraš, S., Trišić, D., Mitrović-Ajtić, O., Antonijević, Đ., Čolović, B., Drobne, D.,& Jokanović, V.. (2021). Biocompatibility Study of a New Dental Cement Based on Hydroxyapatite and Calcium Silicates: Focus on Liver, Kidney and Spleen Tissue Effects. in International Journal of Molecular Science
MDPI., 22(11), 5468.
https://doi.org/10.3390/ijms22115468
Paraš S, Trišić D, Mitrović-Ajtić O, Antonijević Đ, Čolović B, Drobne D, Jokanović V. Biocompatibility Study of a New Dental Cement Based on Hydroxyapatite and Calcium Silicates: Focus on Liver, Kidney and Spleen Tissue Effects. in International Journal of Molecular Science. 2021;22(11):5468.
doi:10.3390/ijms22115468 .
Paraš, Smiljana, Trišić, Dijana, Mitrović-Ajtić, Olivera, Antonijević, Đorđe, Čolović, Božana, Drobne, Damjana, Jokanović, Vukoman, "Biocompatibility Study of a New Dental Cement Based on Hydroxyapatite and Calcium Silicates: Focus on Liver, Kidney and Spleen Tissue Effects" in International Journal of Molecular Science, 22, no. 11 (2021):5468,
https://doi.org/10.3390/ijms22115468 . .
