Еpizootiological-epidemiological importance of the golden jackal (Canis aureus) in the maintenance of vektor-borne zoonoses in the territory of the Republic of Serbia

Abstract

epizootiological and epidemiological role of the golden jackals (Canis aureus) as potential reservoirs for zoonotic vector-borne pathogens, for the first time in Serbia. Jackal spleens and collected ticks were tested by molecular methods (qPCR, conventional PCR, RFLP, sequencing) for the presence of vector-borne pathogens from the genera: Babesia spp., Rickettsia spp., Anaplasma spp., Ehrlichia spp., Francisella spp., Bartonella spp., Leishmania spp., and species from Borrelia burgdorferi sensu lato complex and Coxiella burneti. A total of 216 jackals originating from 10 localities throughout the territory of the Republic of Serbia were analyzed. Beside that, 118 ticks collected from jackals were also tested. The collected ticks belonged to one of three species: Ixodes ricinus (54,2%), Dermacentor reticulatus (39,8%) i Haemaphysalis concinna (5,9%). The presence of DNA of Babesia canis with the prevalence of 4.2% (9/216), Anaplasma phagocytophilum (0.9%, 2/216) and Leishmania spp. (6.9%, 15/216) was confirmed by molecular methods in the spleen of the tested jackals. In ticks collected from jackals, the presence of DNA from two Babesia spp. (Babesia canis, Babesia microti), Anaplasma phagocytophilum and three species from the Borrelia burgdorferi s.l. complex (Borrelia garinii, Borrelia lusitaniae, Borrelia valaisiana) was confirmed...

Istraživanjem sprovedenim u okviru ove doktorske disertacije, po prvi put u Srbiji, analiziran je epizootiološko-epidemiološki značaj šakala (Canis aureus) kao potencijalnih rezervoara za uzročnike zoonoza koje se prenose vektorima. Molekularnim metodama (qPCR, konvencionalni PCR, RFLP, sekvenciranje) u slezinama šakala i krpeljima sakupljenim sa šakala, dokazivano je prisustvo vektorima prenosivih patogena iz rodova: Babesia spp., Rickettsia spp., Anaplasma spp., Ehrlichia spp., Francisella spp., Bartonella spp., Leishmania spp., i vrsta u okviru Borrelia burgdorferi sensu lato kompleksa i Coxiella burneti. Ukupno je analizirano 216 šakala poreklom sa 10 lokaliteta širom teritorije Republike Srbije. Na prisustvo patogena ispitano je i 118 sakupljenih krpelja koji su pripadali jednoj od tri vrste: Ixodes ricinus (54,2%), Dermacentor reticulatus (39,8%) i Haemaphysalis concinna (5,9%). Molekularnim metodama u slezinama šakala potvrđeno je prisustvo DNK Babesia canis sa prevalencijom od 4,2% (9/216), Anaplasma phagocytophilum (0,9%, 2/216) i Leishmania spp. (6,9%, 15/216). U krpeljima sakupljenim sa šakala potvrđeno je prisustvo DNK dve vrste Babesia (Babesia canis, Babesia microti), Anaplasma phagocytophilum i tri vrste Borrelia burgdorferi s.l. kompleksa (Borrelia garinii, Borrelia lusitaniae, Borrelia valaisiana)...