RIMI - Repository of the Institute for Medical Research
Institute for Medical Research
    • English
    • Српски
    • Српски (Serbia)
  • English 
    • English
    • Serbian (Cyrillic)
    • Serbian (Latin)
  • Login
View Item 
  •   RIMI
  • Institut za medicinska istraživanja
  • Doktorati / Doctoral thesis
  • View Item
  •   RIMI
  • Institut za medicinska istraživanja
  • Doktorati / Doctoral thesis
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Effects of different types of diets on the parameters of oxidative stress and the fatty acid profile of rat liver phospholipids

Uticaj različitih vrsta hrane na parametre oksidativnog stresa i masnokiselinski profil fosfolipida jetre pacova

Thumbnail
2018
1117.pdf (4.015Mb)
Authors
Ranković, Slavica G.
Contributors
Popović, Tamara B.
Vujović, Predrag
Lozo, Jelena
Glibetić, Marija D.
Debeljak-Martačić, Jasmina
Doctoral thesis (Published version)
Metadata
Show full item record
Abstract
The composition of fatty acids especially phospholipids (PL) as the main constituents of cell membranes depends on many factors. Recently, there has been an increased interest in novel dietary antioxidants, including omega-3 fatty acids and bioactive proteins present in milk. There is limited data regarding the effects of fatty acid composition of different types of diets on tissue phospholipids fatty acid profile. The aim of this doctoral dissertation was to examine the effects of different types of diets (standard diet, fish based and milk based diets) on plasma biochemical parameters, FA profiles of liver phospholipids, and parameters of oxidative stress in liver, of four months old Wistar rats, during four-weeks. Within this study, fatty acid profile was compared in diets of different composition, as well as in liver phospholipids of animals after treatment. The aim was to detect individual fatty acids mostly contributed to the difference in composition of liver phospholipids, and ...to explore the association of tissue response with gender. The results of this dissertation have shown that both, milk- and fish-based diet, changed the composition and ratio of rat liver phospholipids FA, in gender-specific manner. Although, applied diets changed the ratio of total saturated fatty acids (SFA), monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PUFA), but the effects were sex specific. Milk-based diet lowered the amount of SFA and elevated the ratio of MUFA in males, and PUFA, in females comparing to standard diet. The same diet decreased n-3, increased n-6 and n-6/n-3 ratio in males comparing to the other type of diets. On the other hand, in females, fish-based diet increased n-3, decreased n-6 and n-6/n-3 ratio comparing to standard and milk-based diet. However, the ratio of individual FA in liver PL was also dietary-influenced, but with gender specific manner. While in females fish-based diet decreased AA (arachidonic acid) increased level of EPA (eicosapentaenoic acid), DPA (docosapentaenoic acid) and DHA (docosahexaenoic acid), the same diet elevated only DHA levels in males...

Sastav masnih kiselina lipida, posebno fosfolipida (PL) glavnih konstituenata ćelijskih membrana zavisi od brojnih faktora. U poslednje vreme povećano je interesovanje za istraživanja novih antioksidanasa u ishrani, uključujući omega-3 masne kiseline i bioaktivne proteine prisutne u mleku. Uticaj različitih vrsta hrane, odnosno njihovog masnokiselinskog sastava na profil masnih kiselina fosfolipida tkiva nije dovoljno ispitivan. Stoga, cilj ove doktorske disertacije je bio ispitivanje efekta unosa tri različite vrste hrane (standardne hrane, hrane obogaćene ribljim brašnom i hrane obogaćene mlekom u prahu) na biohemijske parametre u krvi, masnokiselinske profile fosfolipida jetre i na parametre oksidativnog stresa u jetri pacova Wistar soja, starosne dobi četiri meseca u trajanju od četiri nedelje. U okviru ove studije, poređen je manokiselinski profil u hranama različitog sastava, a takođe i u fosfolipidima jetri životinja nakon tretmana. Preciznije, cilj je bio izdvajanje pojedinačni...h masnih kiselina, koje su najviše doprinele razlici u sastavu fosfolipida jetre, kao i ispitivanje polno specifičnog odgovora tkiva na primenjene vrste hrane. Rezultati ove disertacije su pokazali da hrana obogaćena ribljim brašnom i hrana obogaćena mlekom u prahu menja sastav masnih kiselina fosfolipida i njihov odnos u jetri pacova, na polno specifičan način. Primenjene hrane su promenile odnos ukupnih zasićenih (SFA), mononezasićenih (MUFA) i polinezasićenih masnih kiselina (PUFA), ali su efekti polno specifični. Hrana obogaćena mlekom u prahu je smanjila sadržaj SFA i povećala udeo MUFA kod mužjaka, i PUFA kod ženki, u odnosu na standardnu hranu. Ista vrsta hrane je smanjila procenat ukupnih n-3, povećala procenat ukupnih n-6 i odnos n-6/n-3 kod mužjaka u odnosu na druge vrste hrana. S druge strane, kod ženki, hrana obogaćena ribljim brašnom povećala je procenat ukupnih n-3, smanjila procenat ukupnih n-6 i odnos n-6/n-3 u poređenju sa standardnom hranom i hranom obogaćenom mlekom u prahu. Hrana je na polno specifičan način uticala i na odnos pojedinačnih masnih kiselina u PL jetre...

Keywords:
Fish based diet / milk based diet / fatty acids / gender / antioxidant enzymes / lipid peroxidation / Wistar rats / Hrana obogaćena ribljim brašnom / hrana obogaćena mlekom u prahu / masne kiseline / pol / antioksidativni enzimi / lipidna peroksidacija / pacovi
Source:
2018
Publisher:
  • Univerzitet u Beogradu, Biološki fakultet
Funding / projects:
  • Biological effects, nutritional intake and status of folate and polysaturate fatty acid (PUFA): improvement of nutrition in Serbia (RS-41030)
[ Google Scholar ]
Handle
https://hdl.handle.net/21.15107/rcub_nardus_11027
URI
http://eteze.bg.ac.rs/application/showtheses?thesesId=6764
http://nardus.mpn.gov.rs/handle/123456789/11027
https://fedorabg.bg.ac.rs/fedora/get/o:19645/bdef:Content/download
http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025211314
http://rimi.imi.bg.ac.rs/handle/123456789/1120
Collections
  • Doktorati / Doctoral thesis
Institution/Community
Institut za medicinska istraživanja
TY  - THES
AU  - Ranković, Slavica G.
PY  - 2018
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=6764
UR  - http://nardus.mpn.gov.rs/handle/123456789/11027
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:19645/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025211314
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1120
AB  - The composition of fatty acids especially phospholipids (PL) as the main constituents of cell membranes depends on many factors. Recently, there has been an increased interest in novel dietary antioxidants, including omega-3 fatty acids and bioactive proteins present in milk. There is limited data regarding the effects of fatty acid composition of different types of diets on tissue phospholipids fatty acid profile. The aim of this doctoral dissertation was to examine the effects of different types of diets (standard diet, fish based and milk based diets) on plasma biochemical parameters, FA profiles of liver phospholipids, and parameters of oxidative stress in liver, of four months old Wistar rats, during four-weeks. Within this study, fatty acid profile was compared in diets of different composition, as well as in liver phospholipids of animals after treatment. The aim was to detect individual fatty acids mostly contributed to the difference in composition of liver phospholipids, and to explore the association of tissue response with gender. The results of this dissertation have shown that both, milk- and fish-based diet, changed the composition and ratio of rat liver phospholipids FA, in gender-specific manner. Although, applied diets changed the ratio of total saturated fatty acids (SFA), monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PUFA), but the effects were sex specific. Milk-based diet lowered the amount of SFA and elevated the ratio of MUFA in males, and PUFA, in females comparing to standard diet. The same diet decreased n-3, increased n-6 and n-6/n-3 ratio in males comparing to the other type of diets. On the other hand, in females, fish-based diet increased n-3, decreased n-6 and n-6/n-3 ratio comparing to standard and milk-based diet. However, the ratio of individual FA in liver PL was also dietary-influenced, but with gender specific manner. While in females fish-based diet decreased AA (arachidonic acid) increased level of EPA (eicosapentaenoic acid), DPA (docosapentaenoic acid) and DHA (docosahexaenoic acid), the same diet elevated only DHA levels in males...
AB  - Sastav masnih kiselina lipida, posebno fosfolipida (PL) glavnih konstituenata ćelijskih membrana zavisi od brojnih faktora. U poslednje vreme povećano je interesovanje za istraživanja novih antioksidanasa u ishrani, uključujući omega-3 masne kiseline i bioaktivne proteine prisutne u mleku. Uticaj različitih vrsta hrane, odnosno njihovog masnokiselinskog sastava na profil masnih kiselina fosfolipida tkiva nije dovoljno ispitivan. Stoga, cilj ove doktorske disertacije je bio ispitivanje efekta unosa tri različite vrste hrane (standardne hrane, hrane obogaćene ribljim brašnom i hrane obogaćene mlekom u prahu) na biohemijske parametre u krvi, masnokiselinske profile fosfolipida jetre i na parametre oksidativnog stresa u jetri pacova Wistar soja, starosne dobi četiri meseca u trajanju od četiri nedelje. U okviru ove studije, poređen je manokiselinski profil u hranama različitog sastava, a takođe i u fosfolipidima jetri životinja nakon tretmana. Preciznije, cilj je bio izdvajanje pojedinačnih masnih kiselina, koje su najviše doprinele razlici u sastavu fosfolipida jetre, kao i ispitivanje polno specifičnog odgovora tkiva na primenjene vrste hrane. Rezultati ove disertacije su pokazali da hrana obogaćena ribljim brašnom i hrana obogaćena mlekom u prahu menja sastav masnih kiselina fosfolipida i njihov odnos u jetri pacova, na polno specifičan način. Primenjene hrane su promenile odnos ukupnih zasićenih (SFA), mononezasićenih (MUFA) i polinezasićenih masnih kiselina (PUFA), ali su efekti polno specifični. Hrana obogaćena mlekom u prahu je smanjila sadržaj SFA i povećala udeo MUFA kod mužjaka, i PUFA kod ženki, u odnosu na standardnu hranu. Ista vrsta hrane je smanjila procenat ukupnih n-3, povećala procenat ukupnih n-6 i odnos n-6/n-3 kod mužjaka u odnosu na druge vrste hrana. S druge strane, kod ženki, hrana obogaćena ribljim brašnom povećala je procenat ukupnih n-3, smanjila procenat ukupnih n-6 i odnos n-6/n-3 u poređenju sa standardnom hranom i hranom obogaćenom mlekom u prahu. Hrana je na polno specifičan način uticala i na odnos pojedinačnih masnih kiselina u PL jetre...
PB  - Univerzitet u Beogradu, Biološki fakultet
T1  - Effects of different types of diets on the parameters of oxidative stress and the fatty acid profile of rat liver phospholipids
T1  - Uticaj različitih vrsta hrane na parametre oksidativnog stresa i masnokiselinski profil fosfolipida jetre pacova
UR  - https://hdl.handle.net/21.15107/rcub_nardus_11027
ER  - 
@phdthesis{
author = "Ranković, Slavica G.",
year = "2018",
abstract = "The composition of fatty acids especially phospholipids (PL) as the main constituents of cell membranes depends on many factors. Recently, there has been an increased interest in novel dietary antioxidants, including omega-3 fatty acids and bioactive proteins present in milk. There is limited data regarding the effects of fatty acid composition of different types of diets on tissue phospholipids fatty acid profile. The aim of this doctoral dissertation was to examine the effects of different types of diets (standard diet, fish based and milk based diets) on plasma biochemical parameters, FA profiles of liver phospholipids, and parameters of oxidative stress in liver, of four months old Wistar rats, during four-weeks. Within this study, fatty acid profile was compared in diets of different composition, as well as in liver phospholipids of animals after treatment. The aim was to detect individual fatty acids mostly contributed to the difference in composition of liver phospholipids, and to explore the association of tissue response with gender. The results of this dissertation have shown that both, milk- and fish-based diet, changed the composition and ratio of rat liver phospholipids FA, in gender-specific manner. Although, applied diets changed the ratio of total saturated fatty acids (SFA), monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PUFA), but the effects were sex specific. Milk-based diet lowered the amount of SFA and elevated the ratio of MUFA in males, and PUFA, in females comparing to standard diet. The same diet decreased n-3, increased n-6 and n-6/n-3 ratio in males comparing to the other type of diets. On the other hand, in females, fish-based diet increased n-3, decreased n-6 and n-6/n-3 ratio comparing to standard and milk-based diet. However, the ratio of individual FA in liver PL was also dietary-influenced, but with gender specific manner. While in females fish-based diet decreased AA (arachidonic acid) increased level of EPA (eicosapentaenoic acid), DPA (docosapentaenoic acid) and DHA (docosahexaenoic acid), the same diet elevated only DHA levels in males..., Sastav masnih kiselina lipida, posebno fosfolipida (PL) glavnih konstituenata ćelijskih membrana zavisi od brojnih faktora. U poslednje vreme povećano je interesovanje za istraživanja novih antioksidanasa u ishrani, uključujući omega-3 masne kiseline i bioaktivne proteine prisutne u mleku. Uticaj različitih vrsta hrane, odnosno njihovog masnokiselinskog sastava na profil masnih kiselina fosfolipida tkiva nije dovoljno ispitivan. Stoga, cilj ove doktorske disertacije je bio ispitivanje efekta unosa tri različite vrste hrane (standardne hrane, hrane obogaćene ribljim brašnom i hrane obogaćene mlekom u prahu) na biohemijske parametre u krvi, masnokiselinske profile fosfolipida jetre i na parametre oksidativnog stresa u jetri pacova Wistar soja, starosne dobi četiri meseca u trajanju od četiri nedelje. U okviru ove studije, poređen je manokiselinski profil u hranama različitog sastava, a takođe i u fosfolipidima jetri životinja nakon tretmana. Preciznije, cilj je bio izdvajanje pojedinačnih masnih kiselina, koje su najviše doprinele razlici u sastavu fosfolipida jetre, kao i ispitivanje polno specifičnog odgovora tkiva na primenjene vrste hrane. Rezultati ove disertacije su pokazali da hrana obogaćena ribljim brašnom i hrana obogaćena mlekom u prahu menja sastav masnih kiselina fosfolipida i njihov odnos u jetri pacova, na polno specifičan način. Primenjene hrane su promenile odnos ukupnih zasićenih (SFA), mononezasićenih (MUFA) i polinezasićenih masnih kiselina (PUFA), ali su efekti polno specifični. Hrana obogaćena mlekom u prahu je smanjila sadržaj SFA i povećala udeo MUFA kod mužjaka, i PUFA kod ženki, u odnosu na standardnu hranu. Ista vrsta hrane je smanjila procenat ukupnih n-3, povećala procenat ukupnih n-6 i odnos n-6/n-3 kod mužjaka u odnosu na druge vrste hrana. S druge strane, kod ženki, hrana obogaćena ribljim brašnom povećala je procenat ukupnih n-3, smanjila procenat ukupnih n-6 i odnos n-6/n-3 u poređenju sa standardnom hranom i hranom obogaćenom mlekom u prahu. Hrana je na polno specifičan način uticala i na odnos pojedinačnih masnih kiselina u PL jetre...",
publisher = "Univerzitet u Beogradu, Biološki fakultet",
title = "Effects of different types of diets on the parameters of oxidative stress and the fatty acid profile of rat liver phospholipids, Uticaj različitih vrsta hrane na parametre oksidativnog stresa i masnokiselinski profil fosfolipida jetre pacova",
url = "https://hdl.handle.net/21.15107/rcub_nardus_11027"
}
Ranković, S. G.. (2018). Effects of different types of diets on the parameters of oxidative stress and the fatty acid profile of rat liver phospholipids. 
Univerzitet u Beogradu, Biološki fakultet..
https://hdl.handle.net/21.15107/rcub_nardus_11027
Ranković SG. Effects of different types of diets on the parameters of oxidative stress and the fatty acid profile of rat liver phospholipids. 2018;.
https://hdl.handle.net/21.15107/rcub_nardus_11027 .
Ranković, Slavica G., "Effects of different types of diets on the parameters of oxidative stress and the fatty acid profile of rat liver phospholipids" (2018),
https://hdl.handle.net/21.15107/rcub_nardus_11027 .

DSpace software copyright © 2002-2015  DuraSpace
About RIMI | Send Feedback

OpenAIRERCUB
 

 

All of DSpaceCommunitiesAuthorsTitlesSubjectsThis institutionAuthorsTitlesSubjects

Statistics

View Usage Statistics

DSpace software copyright © 2002-2015  DuraSpace
About RIMI | Send Feedback

OpenAIRERCUB