Renal ischemia and reperfusion (I/R) injury is the most common cause of acute kidney injury (AKI). Pathogenesis of postischemic AKI involves hemodynamic changes, oxidative stress, inflammation process, calcium ion overloading, apoptosis and necrosis. Up to date, therapeutic approaches to treat AKI are extremely limited. Thus, the aim of this study was to evaluate the effects of hyperbaric oxygen (HBO) preconditioning on citoprotective enzyme, heme oxygenase-1 (HO-1), pro-apoptotic Bax and anti-apoptotic Bcl-2 proteins expression, in postischemic AKI induced in normotensive Wistar and spontaneously hypertensive rats (SHR). The animals were randomly divided into six experimental groups: SHAM-operated Wistar rats (W-SHAM), Wistar rats with induced postischemic AKI (W-AKI) and Wistar group with HBO preconditioning before AKI induction (W-AKI + HBO). On the other hand, SHR rats were also divided into same three groups: SHR-SHAM, SHR-AKI and SHR-AKI + HBO. We demonstrated that HBO preconditi...oning upregulated HO-1 and anti-apoptotic Bcl-2 protein expression, in both Wistar and SH rats. In addition, HBO preconditioning improved glomerular filtration rate, supporting by significant increase in creatinine, urea and phosphate clearances in both rat strains. Considering our results, we can also say that even in hypertensive conditions, we can expect protective effects of HBO preconditioning in experimental model of AKI.
TY - JOUR
AU - Nešović-Ostojić, Jelena
AU - Ivanov, Milan
AU - Mihailović-Stanojević, Nevena
AU - Karanović, Danijela
AU - Kovacević, Sanjin
AU - Brkić, Predrag
AU - Životić, Maja
AU - Vajić, Una-Jovana
AU - Jovović, Đurđica
AU - Jeremić, Rada
AU - Ljubojević-Holzer, Senka
AU - Miloradović, Zoran
PY - 2021
UR - http://rimi.imi.bg.ac.rs/handle/123456789/1083
AB - Renal ischemia and reperfusion (I/R) injury is the most common cause of acute kidney injury (AKI). Pathogenesis of postischemic AKI involves hemodynamic changes, oxidative stress, inflammation process, calcium ion overloading, apoptosis and necrosis. Up to date, therapeutic approaches to treat AKI are extremely limited. Thus, the aim of this study was to evaluate the effects of hyperbaric oxygen (HBO) preconditioning on citoprotective enzyme, heme oxygenase-1 (HO-1), pro-apoptotic Bax and anti-apoptotic Bcl-2 proteins expression, in postischemic AKI induced in normotensive Wistar and spontaneously hypertensive rats (SHR). The animals were randomly divided into six experimental groups: SHAM-operated Wistar rats (W-SHAM), Wistar rats with induced postischemic AKI (W-AKI) and Wistar group with HBO preconditioning before AKI induction (W-AKI + HBO). On the other hand, SHR rats were also divided into same three groups: SHR-SHAM, SHR-AKI and SHR-AKI + HBO. We demonstrated that HBO preconditioning upregulated HO-1 and anti-apoptotic Bcl-2 protein expression, in both Wistar and SH rats. In addition, HBO preconditioning improved glomerular filtration rate, supporting by significant increase in creatinine, urea and phosphate clearances in both rat strains. Considering our results, we can also say that even in hypertensive conditions, we can expect protective effects of HBO preconditioning in experimental model of AKI.
PB - MDPI, Basel
T2 - International Journal of Molecular Sciences
T1 - Hyperbaric Oxygen Preconditioning Upregulates Heme OxyGenase-1 and Anti-Apoptotic Bcl-2 Protein Expression in Spontaneously Hypertensive Rats with Induced Postischemic Acute Kidney Injury
IS - 3
SP - 1382
VL - 22
DO - 10.3390/ijms22031382
ER -
@article{
author = "Nešović-Ostojić, Jelena and Ivanov, Milan and Mihailović-Stanojević, Nevena and Karanović, Danijela and Kovacević, Sanjin and Brkić, Predrag and Životić, Maja and Vajić, Una-Jovana and Jovović, Đurđica and Jeremić, Rada and Ljubojević-Holzer, Senka and Miloradović, Zoran",
year = "2021",
abstract = "Renal ischemia and reperfusion (I/R) injury is the most common cause of acute kidney injury (AKI). Pathogenesis of postischemic AKI involves hemodynamic changes, oxidative stress, inflammation process, calcium ion overloading, apoptosis and necrosis. Up to date, therapeutic approaches to treat AKI are extremely limited. Thus, the aim of this study was to evaluate the effects of hyperbaric oxygen (HBO) preconditioning on citoprotective enzyme, heme oxygenase-1 (HO-1), pro-apoptotic Bax and anti-apoptotic Bcl-2 proteins expression, in postischemic AKI induced in normotensive Wistar and spontaneously hypertensive rats (SHR). The animals were randomly divided into six experimental groups: SHAM-operated Wistar rats (W-SHAM), Wistar rats with induced postischemic AKI (W-AKI) and Wistar group with HBO preconditioning before AKI induction (W-AKI + HBO). On the other hand, SHR rats were also divided into same three groups: SHR-SHAM, SHR-AKI and SHR-AKI + HBO. We demonstrated that HBO preconditioning upregulated HO-1 and anti-apoptotic Bcl-2 protein expression, in both Wistar and SH rats. In addition, HBO preconditioning improved glomerular filtration rate, supporting by significant increase in creatinine, urea and phosphate clearances in both rat strains. Considering our results, we can also say that even in hypertensive conditions, we can expect protective effects of HBO preconditioning in experimental model of AKI.",
publisher = "MDPI, Basel",
journal = "International Journal of Molecular Sciences",
title = "Hyperbaric Oxygen Preconditioning Upregulates Heme OxyGenase-1 and Anti-Apoptotic Bcl-2 Protein Expression in Spontaneously Hypertensive Rats with Induced Postischemic Acute Kidney Injury",
number = "3",
pages = "1382",
volume = "22",
doi = "10.3390/ijms22031382"
}
Nešović-Ostojić, J., Ivanov, M., Mihailović-Stanojević, N., Karanović, D., Kovacević, S., Brkić, P., Životić, M., Vajić, U., Jovović, Đ., Jeremić, R., Ljubojević-Holzer, S.,& Miloradović, Z.. (2021). Hyperbaric Oxygen Preconditioning Upregulates Heme OxyGenase-1 and Anti-Apoptotic Bcl-2 Protein Expression in Spontaneously Hypertensive Rats with Induced Postischemic Acute Kidney Injury. in International Journal of Molecular Sciences
MDPI, Basel., 22(3), 1382.
https://doi.org/10.3390/ijms22031382
conv_4975
Nešović-Ostojić J, Ivanov M, Mihailović-Stanojević N, Karanović D, Kovacević S, Brkić P, Životić M, Vajić U, Jovović Đ, Jeremić R, Ljubojević-Holzer S, Miloradović Z. Hyperbaric Oxygen Preconditioning Upregulates Heme OxyGenase-1 and Anti-Apoptotic Bcl-2 Protein Expression in Spontaneously Hypertensive Rats with Induced Postischemic Acute Kidney Injury. in International Journal of Molecular Sciences. 2021;22(3):1382.
doi:10.3390/ijms22031382
conv_4975 .
Nešović-Ostojić, Jelena, Ivanov, Milan, Mihailović-Stanojević, Nevena, Karanović, Danijela, Kovacević, Sanjin, Brkić, Predrag, Životić, Maja, Vajić, Una-Jovana, Jovović, Đurđica, Jeremić, Rada, Ljubojević-Holzer, Senka, Miloradović, Zoran, "Hyperbaric Oxygen Preconditioning Upregulates Heme OxyGenase-1 and Anti-Apoptotic Bcl-2 Protein Expression in Spontaneously Hypertensive Rats with Induced Postischemic Acute Kidney Injury" in International Journal of Molecular Sciences, 22, no. 3 (2021):1382,
https://doi.org/10.3390/ijms22031382 .,
conv_4975 .
