Recovery of brain cholinesterases and effect on parameters of oxidative stress and apoptosis in quails (Coturnix japonica) after chlorpyrifos and vitamin B1 administration
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2021
Authors
Ćupić-Miladinović, Dejana![](/themes/MirageRIMI/images/orcid.png)
Prevendar-Crnić, Andreja
Peković, Sanja
Dacić, Sanja
![](/themes/MirageRIMI/images/orcid.png)
Ivanović, Saša
Santibanez, Juan F.
![](/themes/MirageRIMI/images/orcid.png)
Ćupić, Vitomir
Borozan, Nevena
Antonijević-Miljaković, Evica
![](/themes/MirageRIMI/images/orcid.png)
Borozan, Sunčica
![](/themes/MirageRIMI/images/orcid.png)
Article (Published version)
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Chlorpyrifos is a extensively used organophosphate pesticide (OP). In this study, we closely looked into neurotoxicity of CPF and effect of vitamin B1, by checking the levels of cholinesterases, determining the activity of parameters of oxidative stress, inflammation and also level of apoptotic regulator. The study was performed on a total of 80 male Japanese quails (Coturnix japonica), (two control and 6 experimental groups, n = 10). Three group of quails were given by gavage chlorpyrifos (CPF) for 7 consecutive days at doses of 1.50 mg/kg b.w., 3.00 mg/kg b.w., and 6.00 mg/kg b.w. Another three groups were treated with 10 mg/kg b.w. of vitamin B1 i.m. 30 min after CPF application (in above mentioned doses). Our study have proved that all doses of CPF significantly inhibited cholinesterases in brain, while vitamin B1 reactivated them. CPF has led to an increase in the concentration of malondialdehyde (MDA), and activity of catalase (CAT), superoxide dismutase (SOD), glutathione5-trans...ferase (GST), while tiamin changed the activity of antioxidant enzymes: CAT, SOD, GST. CPF stimulated apoptosis by decreasing B-cell lymphoma (Bcl-2) in brain, while application of vitamin B1 caused an increase of this parameter. CPF amplified inflammatory effect by elevating levels of inducible nitric oxide synthase (iNOS), and cyclooxygenase (COX-2). Thiamine proved its anti-inflammatory property by decreasing the expression of iNOS and interleukin-1(IL-1) and interleukin-6(IL-6). This study is highly pertinent because there is little defense currently available to humans and animals to prevent toxic effects of pesticides.
Keywords:
Chlorpyrifos / Vitamin B1 / Japanese quail / Inflammation / Oxidative stress / BrainSource:
Chemico-Biological Interactions, 2021, 333, 109312-Publisher:
- Elsevier
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200143 (University of Belgrade, Faculty of Veterinary Medicine) (RS-MESTD-inst-2020-200143)
DOI: 10.1016/j.cbi.2020.109312
ISSN: 0009-2797
WoS: 000600832900017
Scopus: 2-s2.0-85096159138
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Institut za medicinska istraživanjaTY - JOUR AU - Ćupić-Miladinović, Dejana AU - Prevendar-Crnić, Andreja AU - Peković, Sanja AU - Dacić, Sanja AU - Ivanović, Saša AU - Santibanez, Juan F. AU - Ćupić, Vitomir AU - Borozan, Nevena AU - Antonijević-Miljaković, Evica AU - Borozan, Sunčica PY - 2021 UR - http://rimi.imi.bg.ac.rs/handle/123456789/1073 AB - Chlorpyrifos is a extensively used organophosphate pesticide (OP). In this study, we closely looked into neurotoxicity of CPF and effect of vitamin B1, by checking the levels of cholinesterases, determining the activity of parameters of oxidative stress, inflammation and also level of apoptotic regulator. The study was performed on a total of 80 male Japanese quails (Coturnix japonica), (two control and 6 experimental groups, n = 10). Three group of quails were given by gavage chlorpyrifos (CPF) for 7 consecutive days at doses of 1.50 mg/kg b.w., 3.00 mg/kg b.w., and 6.00 mg/kg b.w. Another three groups were treated with 10 mg/kg b.w. of vitamin B1 i.m. 30 min after CPF application (in above mentioned doses). Our study have proved that all doses of CPF significantly inhibited cholinesterases in brain, while vitamin B1 reactivated them. CPF has led to an increase in the concentration of malondialdehyde (MDA), and activity of catalase (CAT), superoxide dismutase (SOD), glutathione5-transferase (GST), while tiamin changed the activity of antioxidant enzymes: CAT, SOD, GST. CPF stimulated apoptosis by decreasing B-cell lymphoma (Bcl-2) in brain, while application of vitamin B1 caused an increase of this parameter. CPF amplified inflammatory effect by elevating levels of inducible nitric oxide synthase (iNOS), and cyclooxygenase (COX-2). Thiamine proved its anti-inflammatory property by decreasing the expression of iNOS and interleukin-1(IL-1) and interleukin-6(IL-6). This study is highly pertinent because there is little defense currently available to humans and animals to prevent toxic effects of pesticides. PB - Elsevier T2 - Chemico-Biological Interactions T1 - Recovery of brain cholinesterases and effect on parameters of oxidative stress and apoptosis in quails (Coturnix japonica) after chlorpyrifos and vitamin B1 administration SP - 109312 VL - 333 DO - 10.1016/j.cbi.2020.109312 ER -
@article{ author = "Ćupić-Miladinović, Dejana and Prevendar-Crnić, Andreja and Peković, Sanja and Dacić, Sanja and Ivanović, Saša and Santibanez, Juan F. and Ćupić, Vitomir and Borozan, Nevena and Antonijević-Miljaković, Evica and Borozan, Sunčica", year = "2021", abstract = "Chlorpyrifos is a extensively used organophosphate pesticide (OP). In this study, we closely looked into neurotoxicity of CPF and effect of vitamin B1, by checking the levels of cholinesterases, determining the activity of parameters of oxidative stress, inflammation and also level of apoptotic regulator. The study was performed on a total of 80 male Japanese quails (Coturnix japonica), (two control and 6 experimental groups, n = 10). Three group of quails were given by gavage chlorpyrifos (CPF) for 7 consecutive days at doses of 1.50 mg/kg b.w., 3.00 mg/kg b.w., and 6.00 mg/kg b.w. Another three groups were treated with 10 mg/kg b.w. of vitamin B1 i.m. 30 min after CPF application (in above mentioned doses). Our study have proved that all doses of CPF significantly inhibited cholinesterases in brain, while vitamin B1 reactivated them. CPF has led to an increase in the concentration of malondialdehyde (MDA), and activity of catalase (CAT), superoxide dismutase (SOD), glutathione5-transferase (GST), while tiamin changed the activity of antioxidant enzymes: CAT, SOD, GST. CPF stimulated apoptosis by decreasing B-cell lymphoma (Bcl-2) in brain, while application of vitamin B1 caused an increase of this parameter. CPF amplified inflammatory effect by elevating levels of inducible nitric oxide synthase (iNOS), and cyclooxygenase (COX-2). Thiamine proved its anti-inflammatory property by decreasing the expression of iNOS and interleukin-1(IL-1) and interleukin-6(IL-6). This study is highly pertinent because there is little defense currently available to humans and animals to prevent toxic effects of pesticides.", publisher = "Elsevier", journal = "Chemico-Biological Interactions", title = "Recovery of brain cholinesterases and effect on parameters of oxidative stress and apoptosis in quails (Coturnix japonica) after chlorpyrifos and vitamin B1 administration", pages = "109312", volume = "333", doi = "10.1016/j.cbi.2020.109312" }
Ćupić-Miladinović, D., Prevendar-Crnić, A., Peković, S., Dacić, S., Ivanović, S., Santibanez, J. F., Ćupić, V., Borozan, N., Antonijević-Miljaković, E.,& Borozan, S.. (2021). Recovery of brain cholinesterases and effect on parameters of oxidative stress and apoptosis in quails (Coturnix japonica) after chlorpyrifos and vitamin B1 administration. in Chemico-Biological Interactions Elsevier., 333, 109312. https://doi.org/10.1016/j.cbi.2020.109312
Ćupić-Miladinović D, Prevendar-Crnić A, Peković S, Dacić S, Ivanović S, Santibanez JF, Ćupić V, Borozan N, Antonijević-Miljaković E, Borozan S. Recovery of brain cholinesterases and effect on parameters of oxidative stress and apoptosis in quails (Coturnix japonica) after chlorpyrifos and vitamin B1 administration. in Chemico-Biological Interactions. 2021;333:109312. doi:10.1016/j.cbi.2020.109312 .
Ćupić-Miladinović, Dejana, Prevendar-Crnić, Andreja, Peković, Sanja, Dacić, Sanja, Ivanović, Saša, Santibanez, Juan F., Ćupić, Vitomir, Borozan, Nevena, Antonijević-Miljaković, Evica, Borozan, Sunčica, "Recovery of brain cholinesterases and effect on parameters of oxidative stress and apoptosis in quails (Coturnix japonica) after chlorpyrifos and vitamin B1 administration" in Chemico-Biological Interactions, 333 (2021):109312, https://doi.org/10.1016/j.cbi.2020.109312 . .